ID   SYQ_PSEAE               Reviewed;         556 AA.
AC   Q9I2U8;
DT   02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   27-MAR-2024, entry version 132.
DE   RecName: Full=Glutamine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00126};
DE            EC=6.1.1.18 {ECO:0000255|HAMAP-Rule:MF_00126};
DE   AltName: Full=Glutaminyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00126};
DE            Short=GlnRS {ECO:0000255|HAMAP-Rule:MF_00126};
GN   Name=glnS {ECO:0000255|HAMAP-Rule:MF_00126}; OrderedLocusNames=PA1794;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 /
RC   1C / PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-
CC         glutaminyl-tRNA(Gln); Xref=Rhea:RHEA:20121, Rhea:RHEA-COMP:9662,
CC         Rhea:RHEA-COMP:9681, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58359, ChEBI:CHEBI:78442, ChEBI:CHEBI:78521,
CC         ChEBI:CHEBI:456215; EC=6.1.1.18; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00126};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00126}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00126}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00126}.
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DR   EMBL; AE004091; AAG05183.1; -; Genomic_DNA.
DR   PIR; F83421; F83421.
DR   RefSeq; NP_250485.1; NC_002516.2.
DR   RefSeq; WP_003113586.1; NZ_QZGE01000003.1.
DR   PDB; 5BNZ; X-ray; 1.90 A; A/B=1-556.
DR   PDBsum; 5BNZ; -.
DR   AlphaFoldDB; Q9I2U8; -.
DR   SMR; Q9I2U8; -.
DR   STRING; 208964.PA1794; -.
DR   PaxDb; 208964-PA1794; -.
DR   GeneID; 878828; -.
DR   KEGG; pae:PA1794; -.
DR   PATRIC; fig|208964.12.peg.1861; -.
DR   PseudoCAP; PA1794; -.
DR   HOGENOM; CLU_001882_2_3_6; -.
DR   InParanoid; Q9I2U8; -.
DR   OrthoDB; 9801560at2; -.
DR   PhylomeDB; Q9I2U8; -.
DR   BioCyc; PAER208964:G1FZ6-1826-MONOMER; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004819; F:glutamine-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0006425; P:glutaminyl-tRNA aminoacylation; IBA:GO_Central.
DR   GO; GO:0006424; P:glutamyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd00807; GlnRS_core; 1.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   HAMAP; MF_00126; Gln_tRNA_synth; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR004514; Gln-tRNA-synth.
DR   InterPro; IPR022861; Gln_tRNA_ligase_bac.
DR   InterPro; IPR000924; Glu/Gln-tRNA-synth.
DR   InterPro; IPR020058; Glu/Gln-tRNA-synth_Ib_cat-dom.
DR   InterPro; IPR020059; Glu/Gln-tRNA-synth_Ib_codon-bd.
DR   InterPro; IPR020061; Glu_tRNA_lig_a-bdl.
DR   InterPro; IPR020056; Rbsml_bL25/Gln-tRNA_synth_N.
DR   InterPro; IPR011035; Ribosomal_bL25/Gln-tRNA_synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR049437; tRNA-synt_1c_C2.
DR   NCBIfam; TIGR00440; glnS; 1.
DR   PANTHER; PTHR43097:SF4; GLUTAMINE--TRNA LIGASE; 1.
DR   PANTHER; PTHR43097; GLUTAMINE-TRNA LIGASE; 1.
DR   Pfam; PF00749; tRNA-synt_1c; 1.
DR   Pfam; PF03950; tRNA-synt_1c_C; 1.
DR   Pfam; PF20974; tRNA-synt_1c_C2; 1.
DR   PRINTS; PR00987; TRNASYNTHGLU.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR   SUPFAM; SSF50715; Ribosomal protein L25-like; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..556
FT                   /note="Glutamine--tRNA ligase"
FT                   /id="PRO_0000195843"
FT   MOTIF           35..45
FT                   /note="'HIGH' region"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT   MOTIF           269..273
FT                   /note="'KMSKS' region"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT   BINDING         36..38
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT   BINDING         42..48
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT   BINDING         68
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT   BINDING         213
FT                   /ligand="L-glutamine"
FT                   /ligand_id="ChEBI:CHEBI:58359"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT   BINDING         232
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT   BINDING         262..263
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT   HELIX           12..22
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          30..33
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          37..39
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           43..58
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          62..67
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           76..89
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          95..97
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           101..104
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           105..117
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          121..124
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           128..135
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          138..140
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   TURN            146..149
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           152..163
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          173..176
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           185..187
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          191..195
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   TURN            201..203
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          208..211
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           213..224
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          227..232
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           233..238
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           239..248
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          256..260
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           272..280
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          283..286
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          292..294
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           295..301
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           305..315
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           327..340
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          346..355
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          363..370
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          372..374
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          378..390
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           391..393
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          410..413
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          418..426
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          432..440
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          460..462
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          466..473
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          476..481
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           491..494
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          499..508
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   HELIX           510..514
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          520..523
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   TURN            524..526
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          527..531
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   TURN            533..535
FT                   /evidence="ECO:0007829|PDB:5BNZ"
FT   STRAND          543..547
FT                   /evidence="ECO:0007829|PDB:5BNZ"
SQ   SEQUENCE   556 AA;  62913 MW;  EE75B1BB2C0A77F1 CRC64;
     MSKPETTAAP NFLRQIVQAD LDAGKHAKIV TRFPPEPNGY LHIGHAKSIC LNFGLAQEFA
     GDCHLRFDDT NPAKEDQEYI DAIEADIKWL GFQWSGEVCY ASNYFDQLHA WAVELIKAGK
     AFVCDLGPEE MREYRGTLTE PGRNSPYRDR SVEENLDLFA RMKAGEFPDG ARSLRAKIDM
     GSPNMNLRDP ILYRIRHAHH HQTGDKWCIY PSYDFTHGQS DAIEGITHSI CTLEFEDHRP
     LYEWFLANLP VPAQPRQYEF SRLNLNYTVT SKRKLKQLVD EGHVSGWDDP RMSTLSGYRR
     RGYTPESIRN FCEMIGVNRA SGVVDIGMLE FSIRDHLDAT APRAMCVLKP LKVVITNYPE
     GQVENLELPR HPKEDMGVRV LPFGRELFID AGDFEEVPPA GYKRLIPGGE VRLRGSYVIR
     ADEAIKDADG NIVELRCSYD PDTLGKNPEG RKVKGVIHWV PAEGSVECEV RLYDRLFRSA
     NPEKAEEGGS FLDNINADSL QVLAGCRAEP SLGQANPEDR FQFEREGYFV ADLKDSRPGK
     PVFNRTVTLR DSWGQG
//