Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamine--tRNA ligase

Gene

glnS

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei272ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligaseUniRule annotation (EC:6.1.1.18UniRule annotation)
Alternative name(s):
Glutaminyl-tRNA synthetaseUniRule annotation
Short name:
GlnRSUniRule annotation
Gene namesi
Name:glnSUniRule annotation
Ordered Locus Names:PA1794
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1794.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001958431 – 556Glutamine--tRNA ligaseAdd BLAST556

Proteomic databases

PaxDbiQ9I2U8.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA1794.

Structurei

Secondary structure

1556
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 22Combined sources11
Beta strandi30 – 33Combined sources4
Beta strandi37 – 39Combined sources3
Helixi43 – 58Combined sources16
Beta strandi62 – 67Combined sources6
Helixi76 – 89Combined sources14
Beta strandi95 – 97Combined sources3
Helixi101 – 104Combined sources4
Helixi105 – 117Combined sources13
Beta strandi121 – 124Combined sources4
Helixi128 – 135Combined sources8
Beta strandi138 – 140Combined sources3
Turni146 – 149Combined sources4
Helixi152 – 163Combined sources12
Beta strandi173 – 176Combined sources4
Helixi185 – 187Combined sources3
Beta strandi191 – 195Combined sources5
Turni201 – 203Combined sources3
Beta strandi208 – 211Combined sources4
Helixi213 – 224Combined sources12
Beta strandi227 – 232Combined sources6
Helixi233 – 238Combined sources6
Helixi239 – 248Combined sources10
Beta strandi256 – 260Combined sources5
Helixi272 – 280Combined sources9
Beta strandi283 – 286Combined sources4
Beta strandi292 – 294Combined sources3
Helixi295 – 301Combined sources7
Helixi305 – 315Combined sources11
Helixi327 – 340Combined sources14
Beta strandi346 – 355Combined sources10
Beta strandi363 – 370Combined sources8
Beta strandi372 – 374Combined sources3
Beta strandi378 – 390Combined sources13
Helixi391 – 393Combined sources3
Beta strandi410 – 413Combined sources4
Beta strandi418 – 426Combined sources9
Beta strandi432 – 440Combined sources9
Beta strandi460 – 462Combined sources3
Beta strandi466 – 473Combined sources8
Beta strandi476 – 481Combined sources6
Helixi491 – 494Combined sources4
Beta strandi499 – 508Combined sources10
Helixi510 – 514Combined sources5
Beta strandi520 – 523Combined sources4
Turni524 – 526Combined sources3
Beta strandi527 – 531Combined sources5
Turni533 – 535Combined sources3
Beta strandi543 – 547Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5BNZX-ray1.90A/B1-556[»]
ProteinModelPortaliQ9I2U8.
SMRiQ9I2U8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi35 – 45"HIGH" regionAdd BLAST11
Motifi269 – 273"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CX6. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000259232.
InParanoidiQ9I2U8.
KOiK01886.
OMAiHCTYDPE.
PhylomeDBiQ9I2U8.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I2U8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPETTAAP NFLRQIVQAD LDAGKHAKIV TRFPPEPNGY LHIGHAKSIC
60 70 80 90 100
LNFGLAQEFA GDCHLRFDDT NPAKEDQEYI DAIEADIKWL GFQWSGEVCY
110 120 130 140 150
ASNYFDQLHA WAVELIKAGK AFVCDLGPEE MREYRGTLTE PGRNSPYRDR
160 170 180 190 200
SVEENLDLFA RMKAGEFPDG ARSLRAKIDM GSPNMNLRDP ILYRIRHAHH
210 220 230 240 250
HQTGDKWCIY PSYDFTHGQS DAIEGITHSI CTLEFEDHRP LYEWFLANLP
260 270 280 290 300
VPAQPRQYEF SRLNLNYTVT SKRKLKQLVD EGHVSGWDDP RMSTLSGYRR
310 320 330 340 350
RGYTPESIRN FCEMIGVNRA SGVVDIGMLE FSIRDHLDAT APRAMCVLKP
360 370 380 390 400
LKVVITNYPE GQVENLELPR HPKEDMGVRV LPFGRELFID AGDFEEVPPA
410 420 430 440 450
GYKRLIPGGE VRLRGSYVIR ADEAIKDADG NIVELRCSYD PDTLGKNPEG
460 470 480 490 500
RKVKGVIHWV PAEGSVECEV RLYDRLFRSA NPEKAEEGGS FLDNINADSL
510 520 530 540 550
QVLAGCRAEP SLGQANPEDR FQFEREGYFV ADLKDSRPGK PVFNRTVTLR

DSWGQG
Length:556
Mass (Da):62,913
Last modified:March 1, 2001 - v1
Checksum:iEE75B1BB2C0A77F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG05183.1.
PIRiF83421.
RefSeqiNP_250485.1. NC_002516.2.
WP_003113586.1. NZ_ASJY01000250.1.

Genome annotation databases

EnsemblBacteriaiAAG05183; AAG05183; PA1794.
GeneIDi878828.
KEGGipae:PA1794.
PATRICi19837951. VBIPseAer58763_1861.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG05183.1.
PIRiF83421.
RefSeqiNP_250485.1. NC_002516.2.
WP_003113586.1. NZ_ASJY01000250.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5BNZX-ray1.90A/B1-556[»]
ProteinModelPortaliQ9I2U8.
SMRiQ9I2U8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1794.

Proteomic databases

PaxDbiQ9I2U8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG05183; AAG05183; PA1794.
GeneIDi878828.
KEGGipae:PA1794.
PATRICi19837951. VBIPseAer58763_1861.

Organism-specific databases

PseudoCAPiPA1794.

Phylogenomic databases

eggNOGiENOG4105CX6. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000259232.
InParanoidiQ9I2U8.
KOiK01886.
OMAiHCTYDPE.
PhylomeDBiQ9I2U8.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYQ_PSEAE
AccessioniPrimary (citable) accession number: Q9I2U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.