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Protein

Bifunctional protein FolD

Gene

folD

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation1 Publication

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation1 Publication
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation1 Publication

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei232NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 168NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:PA1796
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1796.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002684431 – 284Bifunctional protein FolDAdd BLAST284

Proteomic databases

PaxDbiQ9I2U6.
PRIDEiQ9I2U6.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi208964.PA1796.

Structurei

Secondary structure

1284
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 28Combined sources21
Beta strandi35 – 42Combined sources8
Helixi45 – 60Combined sources16
Beta strandi64 – 70Combined sources7
Helixi76 – 87Combined sources12
Beta strandi94 – 97Combined sources4
Helixi107 – 112Combined sources6
Helixi116 – 118Combined sources3
Helixi125 – 132Combined sources8
Helixi141 – 152Combined sources12
Beta strandi161 – 165Combined sources5
Helixi172 – 181Combined sources10
Beta strandi185 – 189Combined sources5
Helixi196 – 201Combined sources6
Beta strandi204 – 208Combined sources5
Helixi218 – 220Combined sources3
Beta strandi226 – 229Combined sources4
Helixi246 – 252Combined sources7
Beta strandi254 – 256Combined sources3
Helixi263 – 281Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4A5OX-ray2.20A/B/C/D1-284[»]
ProteinModelPortaliQ9I2U6.
SMRiQ9I2U6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
InParanoidiQ9I2U6.
KOiK01491.
OMAiLMFNTIK.
PhylomeDBiQ9I2U6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I2U6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAQLIDGKA IAANLRQQIA QRVTERRQQG LRVPGLAVIL VGTDPASQVY
60 70 80 90 100
VAHKRKDCEE VGFLSQAYDL PAETSQDDLL ALIDRLNDDP AIDGILVQLP
110 120 130 140 150
LPAHLDASLL LERIHPDKDV DGFHPYNIGR LAQRMPLLRP CTPKGIMTLL
160 170 180 190 200
ASTGADLYGM DAVVVGASNI VGRPMALELL LGGCTVTVTH RFTRDLADHV
210 220 230 240 250
SRADLVVVAA GKPGLVKGEW IKEGAIVIDV GINRQADGRL VGDVEYEVAA
260 270 280
QRASWITPVP GGVGPMTRAC LLENTLHAAE HLHD
Length:284
Mass (Da):30,549
Last modified:March 1, 2001 - v1
Checksum:i22657CF7BEDE7967
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG05185.1.
PIRiH83421.
RefSeqiNP_250487.1. NC_002516.2.
WP_003087898.1. NZ_ASJY01000250.1.

Genome annotation databases

EnsemblBacteriaiAAG05185; AAG05185; PA1796.
GeneIDi880636.
KEGGipae:PA1796.
PATRICi19837955. VBIPseAer58763_1863.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG05185.1.
PIRiH83421.
RefSeqiNP_250487.1. NC_002516.2.
WP_003087898.1. NZ_ASJY01000250.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4A5OX-ray2.20A/B/C/D1-284[»]
ProteinModelPortaliQ9I2U6.
SMRiQ9I2U6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1796.

Proteomic databases

PaxDbiQ9I2U6.
PRIDEiQ9I2U6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG05185; AAG05185; PA1796.
GeneIDi880636.
KEGGipae:PA1796.
PATRICi19837955. VBIPseAer58763_1863.

Organism-specific databases

PseudoCAPiPA1796.

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
InParanoidiQ9I2U6.
KOiK01491.
OMAiLMFNTIK.
PhylomeDBiQ9I2U6.

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOLD_PSEAE
AccessioniPrimary (citable) accession number: Q9I2U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.