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Protein

Bifunctional protein FolD

Gene

folD

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation1 Publication

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation1 Publication
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation1 Publication

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei232NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 168NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-1828-MONOMER
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:PA1796
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1796

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002684431 – 284Bifunctional protein FolDAdd BLAST284

Proteomic databases

PaxDbiQ9I2U6

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi208964.PA1796

Structurei

Secondary structure

1284
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 28Combined sources21
Beta strandi35 – 42Combined sources8
Helixi45 – 60Combined sources16
Beta strandi64 – 70Combined sources7
Helixi76 – 87Combined sources12
Beta strandi94 – 97Combined sources4
Helixi107 – 112Combined sources6
Helixi116 – 118Combined sources3
Helixi125 – 132Combined sources8
Helixi141 – 152Combined sources12
Beta strandi161 – 165Combined sources5
Helixi172 – 181Combined sources10
Beta strandi185 – 189Combined sources5
Helixi196 – 201Combined sources6
Beta strandi204 – 208Combined sources5
Helixi218 – 220Combined sources3
Beta strandi226 – 229Combined sources4
Helixi246 – 252Combined sources7
Beta strandi254 – 256Combined sources3
Helixi263 – 281Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4A5OX-ray2.20A/B/C/D1-284[»]
ProteinModelPortaliQ9I2U6
SMRiQ9I2U6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0 Bacteria
COG0190 LUCA
HOGENOMiHOG000218242
InParanoidiQ9I2U6
KOiK01491
OMAiAGKLCGD
PhylomeDBiQ9I2U6

Family and domain databases

HAMAPiMF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020867 THF_DH/CycHdrlase_CS
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PANTHERiPTHR10025 PTHR10025, 1 hit
PfamiView protein in Pfam
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00766 THF_DHG_CYH_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9I2U6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAQLIDGKA IAANLRQQIA QRVTERRQQG LRVPGLAVIL VGTDPASQVY
60 70 80 90 100
VAHKRKDCEE VGFLSQAYDL PAETSQDDLL ALIDRLNDDP AIDGILVQLP
110 120 130 140 150
LPAHLDASLL LERIHPDKDV DGFHPYNIGR LAQRMPLLRP CTPKGIMTLL
160 170 180 190 200
ASTGADLYGM DAVVVGASNI VGRPMALELL LGGCTVTVTH RFTRDLADHV
210 220 230 240 250
SRADLVVVAA GKPGLVKGEW IKEGAIVIDV GINRQADGRL VGDVEYEVAA
260 270 280
QRASWITPVP GGVGPMTRAC LLENTLHAAE HLHD
Length:284
Mass (Da):30,549
Last modified:March 1, 2001 - v1
Checksum:i22657CF7BEDE7967
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG05185.1
PIRiH83421
RefSeqiNP_250487.1, NC_002516.2
WP_003087898.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG05185; AAG05185; PA1796
GeneIDi880636
KEGGipae:PA1796
PATRICifig|208964.12.peg.1863

Similar proteinsi

Entry informationi

Entry nameiFOLD_PSEAE
AccessioniPrimary (citable) accession number: Q9I2U6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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