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Q9I2Q2

- METH_PSEAE

UniProt

Q9I2Q2 - METH_PSEAE

Protein

Methionine synthase

Gene

metH

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate By similarity.By similarity

    Catalytic activityi

    5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine.

    Cofactori

    Methylcobalamin (MeCBL).By similarity
    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi254 – 2541ZincPROSITE-ProRule annotation
    Metal bindingi317 – 3171ZincPROSITE-ProRule annotation
    Metal bindingi318 – 3181ZincPROSITE-ProRule annotation
    Metal bindingi765 – 7651Cobalt (cobalamin axial ligand)By similarity
    Binding sitei810 – 8101CobalaminBy similarity
    Binding sitei954 – 9541S-adenosyl-L-methionineBy similarity
    Binding sitei1142 – 11421S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei1146 – 11461Cobalamin; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. cobalamin binding Source: UniProtKB-KW
    2. methionine synthase activity Source: UniProtKB-EC
    3. S-adenosylmethionine-homocysteine S-methyltransferase activity Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. pteridine-containing compound metabolic process Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    Cobalamin, Cobalt, Metal-binding, S-adenosyl-L-methionine, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00051; UER00081.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine synthase (EC:2.1.1.13)
    Alternative name(s):
    5-methyltetrahydrofolate--homocysteine methyltransferase
    Methionine synthase, vitamin-B12 dependent
    Short name:
    MS
    Gene namesi
    Name:metH
    Ordered Locus Names:PA1843
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA1843.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12341234Methionine synthasePRO_0000287780Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA1843.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9I2Q2.
    SMRiQ9I2Q2. Positions 19-646, 658-1233.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 332321Hcy-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini363 – 624262Pterin-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini655 – 74995B12-binding N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini752 – 888137B12-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini904 – 1234331AdoMet activationPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni840 – 8412Cobalamin-bindingBy similarity
    Regioni1197 – 11982S-adenosyl-L-methionine bindingBy similarity

    Domaini

    Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin By similarity.By similarity

    Sequence similaritiesi

    Contains 1 AdoMet activation domain.PROSITE-ProRule annotation
    Contains 1 B12-binding domain.PROSITE-ProRule annotation
    Contains 1 Hcy-binding domain.PROSITE-ProRule annotation
    Contains 1 pterin-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1410.
    HOGENOMiHOG000251409.
    KOiK00548.
    OMAiLTEHYAM.
    OrthoDBiEOG6091CH.
    PhylomeDBiQ9I2Q2.

    Family and domain databases

    Gene3Di1.10.1240.10. 1 hit.
    3.10.196.10. 1 hit.
    3.20.20.20. 1 hit.
    3.20.20.330. 1 hit.
    3.40.50.280. 1 hit.
    InterProiIPR003759. Cbl-bd_cap.
    IPR006158. Cobalamin-bd.
    IPR011005. Dihydropteroate_synth-like.
    IPR011822. MetH.
    IPR000489. Pterin-binding.
    IPR003726. S_MeTrfase.
    IPR004223. VitB12-dep_Met_synth_activ_dom.
    [Graphical view]
    PfamiPF02310. B12-binding. 1 hit.
    PF02607. B12-binding_2. 1 hit.
    PF02965. Met_synt_B12. 1 hit.
    PF00809. Pterin_bind. 1 hit.
    PF02574. S-methyl_trans. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000381. MetH. 1 hit.
    SMARTiSM01018. B12-binding_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47644. SSF47644. 1 hit.
    SSF51717. SSF51717. 1 hit.
    SSF52242. SSF52242. 1 hit.
    SSF56507. SSF56507. 1 hit.
    SSF82282. SSF82282. 1 hit.
    TIGRFAMsiTIGR02082. metH. 1 hit.
    PROSITEiPS50974. ADOMET_ACTIVATION. 1 hit.
    PS51332. B12_BINDING. 1 hit.
    PS51337. B12_BINDING_NTER. 1 hit.
    PS50970. HCY. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9I2Q2-1 [UniParc]FASTAAdd to Basket

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    MSSPLTDRSA RLQALQHALR ERILILDGGM GTMIQSYKLE EADYRGERFA     50
    DWPSDVKGNN DLLLLSRPDV IQAIEKAYLD AGADILETNT FNATQVSQAD 100
    YGMQSLAYEL NVEGARLARQ VADAKTAETP DKPRFVAGVL GPTSRTCSIS 150
    PDVNNPGYRN VTFDELVENY VEATRGLIEG GADLILIETI FDTLNAKAAI 200
    FAVQGVFEEL GVELPIMISG TITDASGRTL SGQTTEAFWN SVRHARPISV 250
    GLNCALGAKE LRPYIEELST KADTHVSAHP NAGLPNAFGE YDESPAEMAV 300
    VVEEFAAAGF LNIVGGCCGT TPAHIEAIAK AVAKYPPRAI PEIPRACRLS 350
    GLEPFTIDRS SLFVNVGERT NITGSAKFAR LIREENYAEA LEVAQQQVEA 400
    GAQVIDINMD EGMLDSKAAM VTFLNLIASE PDISRVPIMI DSSKWEVIEA 450
    GLKCIQGKGI VNSISMKEGV EAFKHHARLC KRYGAAVVVM AFDEDGQADT 500
    QARKEEICKR SYDILVDEVG FPPEDIIFDA NIFAIATGIE EHNNYAVDFI 550
    NACAYIRDNL PYALSSGGVS NVSFSFRGNN PVREAIHSVF LYYAIRNGLT 600
    MGIVNAGQLE IYDEIPKALR DRVEDVVLNR TPEATEALLA IADDYKGGGA 650
    VKEAEDEEWR SYSVEKRLEH ALVKGITTWI VEDTEECRQQ CARPIEVIEG 700
    PLMSGMNVVG DLFGAGKMFL PQVVKSARVM KQAVAHLIPF IEAEKGDKPE 750
    AKGKILMATV KGDVHDIGKN IVGVVLGCNG YDVVDLGVMV PAEKILQTAI 800
    AEKCDIIGLS GLITPSLDEM VHVAKEMQRQ NFQLPLMIGG ATTSKAHTAV 850
    KIDPQYSNDA VVYVTDASRA VGVATSLLSK ELKADYVART RADYAVVRER 900
    TANRSARTER LSYEQAIANK PAFDWAGYQA PTPSFTGVRV LDEIDLAVLA 950
    EYIDWTPFFI SWDLAGKYPR ILTDEVVGEA ATSLFNDAQA MLKKLIDEKL 1000
    IKARAVFGFW PANQVEHDDL EVYGADGETL ATLHHLRQQT IKPDGKPNLS 1050
    LADFVAPKES GVRDYIGGFI TTAGIGAEEV AKAYEAKGDD YNSIMVKALA 1100
    DRLAEACAEW LHERVRKEYW GYARDEHLDN EALIKEQYVG IRPAPGYPAC 1150
    PDHTEKGTLF ELLDPQGLSG VSLTEHYAMF PAAAVSGWYF AHPQAQYFAV 1200
    GKIDKDQVER YSQRKGQEAS VSERWLAPNL GYDD 1234
    Length:1,234
    Mass (Da):135,063
    Last modified:March 1, 2001 - v1
    Checksum:i9877F8AC8BEF44ED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG05232.1.
    PIRiE83415.
    RefSeqiNP_250534.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG05232; AAG05232; PA1843.
    GeneIDi880631.
    KEGGipae:PA1843.
    PATRICi19838071. VBIPseAer58763_1916.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG05232.1 .
    PIRi E83415.
    RefSeqi NP_250534.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali Q9I2Q2.
    SMRi Q9I2Q2. Positions 19-646, 658-1233.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA1843.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG05232 ; AAG05232 ; PA1843 .
    GeneIDi 880631.
    KEGGi pae:PA1843.
    PATRICi 19838071. VBIPseAer58763_1916.

    Organism-specific databases

    PseudoCAPi PA1843.

    Phylogenomic databases

    eggNOGi COG1410.
    HOGENOMi HOG000251409.
    KOi K00548.
    OMAi LTEHYAM.
    OrthoDBi EOG6091CH.
    PhylomeDBi Q9I2Q2.

    Enzyme and pathway databases

    UniPathwayi UPA00051 ; UER00081 .

    Family and domain databases

    Gene3Di 1.10.1240.10. 1 hit.
    3.10.196.10. 1 hit.
    3.20.20.20. 1 hit.
    3.20.20.330. 1 hit.
    3.40.50.280. 1 hit.
    InterProi IPR003759. Cbl-bd_cap.
    IPR006158. Cobalamin-bd.
    IPR011005. Dihydropteroate_synth-like.
    IPR011822. MetH.
    IPR000489. Pterin-binding.
    IPR003726. S_MeTrfase.
    IPR004223. VitB12-dep_Met_synth_activ_dom.
    [Graphical view ]
    Pfami PF02310. B12-binding. 1 hit.
    PF02607. B12-binding_2. 1 hit.
    PF02965. Met_synt_B12. 1 hit.
    PF00809. Pterin_bind. 1 hit.
    PF02574. S-methyl_trans. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000381. MetH. 1 hit.
    SMARTi SM01018. B12-binding_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47644. SSF47644. 1 hit.
    SSF51717. SSF51717. 1 hit.
    SSF52242. SSF52242. 1 hit.
    SSF56507. SSF56507. 1 hit.
    SSF82282. SSF82282. 1 hit.
    TIGRFAMsi TIGR02082. metH. 1 hit.
    PROSITEi PS50974. ADOMET_ACTIVATION. 1 hit.
    PS51332. B12_BINDING. 1 hit.
    PS51337. B12_BINDING_NTER. 1 hit.
    PS50970. HCY. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiMETH_PSEAE
    AccessioniPrimary (citable) accession number: Q9I2Q2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    L-homocysteine is bound via the zinc atom.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3