Reviewed, UniProtKB/Swiss-Prot Q9I2A8 (ATOB_PSEAE)

Names and origin

Protein names

Recommended name:
    Acetyl-CoA acetyltransferase
    EC=2.3.1.9
Alternative name(s):
    Acetoacetyl-CoA thiolase

Gene names

Name:	atoB
Ordered Locus Names:	PA2001

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Acetyl-CoA acetyltransferase		PRO_0000287828
Sites
	Active site	88	1	Acyl-thioester intermediate By similarity
	Active site	349	1	Proton acceptor By similarity
	Active site	379	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I2A8-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 4BFFEA8F1D8352B8	FASTA	393	40,405
	10 20 30 40 50 60 MQDVVIVAAT RTAVGSFQGS LAGIPAPELG AAVIRRLLEQ TGLDAGQVDE VILGQVLTAG 70 80 90 100 110 120 SGQNPARQAA IKAGLPVGVP AMTLNKVCGS GLKALHLGAQ AIRCGDAEVI VAGGQENMSL 130 140 150 160 170 180 APYVMPGART GLRMGHAKLV DSMIEDGLWD AFNDYHMGIT AENLAEKYGL TREEQDAFAA 190 200 210 220 230 240 ASQQKAIAAI EGGRFRDEIT PIQVPQRKGE PLGFDTDEQP RAGTTVEALA KLKPAFRKDG 250 260 270 280 290 300 SVTAGNASSL NDGAAAVLLM SAAKAKALGL PVLARIASYA SAGVDPAIMG IGPVSATRRA 310 320 330 340 350 360 LDKAGWSLEQ LDLIEANEAF AAQSLAVGRE LGWDAARVNV NGGAIALGHP IGASGCRVLV 370 380 390 TLLHEMIRRD AKKGLATLCI GGGQGVALTL ARD

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG05389.1.
PIR	C83396.
RefSeq	NP_250691.1.
3D structure databases
HSSP	HSSP built from PDB template 1M3K based on UniProtKB P07097.
SMR	Q9I2A8. Positions 4-390.
ModBase	Search...
Genome annotation databases
GeneID	879046.
GenomeReviews	Gene locus PA2001 in contig AE004091_GR.
KEGG	pae:PA2001.
NMPDR	fig|208964.1.peg.2001.
Organism-specific databases
PseudoCAP	PA2001.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG370930.
OMA	MQDEFAV.
Enzyme and pathway databases
BioCyc	PAER208964:PA2001-MONOMER.
BRENDA	2.3.1.9. 354.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATOB_PSEAE

Accession

Primary (citable) accession number: Q9I2A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)