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Q9I1W2 (GLGB_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:PA2153
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length732 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7327321,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188730

Sites

Active site4121Nucleophile By similarity
Active site4651Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9I1W2 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: C31303D6D5F929F4

FASTA73282,563
        10         20         30         40         50         60 
MSEPMSERER WQLELDKLQR GLQGDPFAFL GPQRDPGGEG GVLRAYLPGA QRVELLDEDG 

        70         80         90        100        110        120 
ATLAELEQSD PGSGLFQRHL ERLPPRYRLR VHWPDGVQES EDPYAFGPLL GELDLYLFAE 

       130        140        150        160        170        180 
GNHRQLASCL GAQLTRHEGV EGVRFAVWAP NAVRVSVVGD FNGWDGRRHP MRRRYPSGVW 

       190        200        210        220        230        240 
ELFVPRLGEG ELYKYELQGH DGLLPLKADP LALACETPPG TASKTCAALR HEWRDQDWLA 

       250        260        270        280        290        300 
RRAERQGYAA PLSIYEVHAG SWRHRDGRPP HWSELAEELI PYVRQLGFTH IELMPVMEHP 

       310        320        330        340        350        360 
FGGSWGYQPL GLFAPTARYG TPEDFAAFVD ACHQAGIGVI LDWVPAHFPT DAHGLGRFDG 

       370        380        390        400        410        420 
TALYEYEHPF EGFHQDWDTY IYNLGRSEVH GFMLASALHW LRTYHVDGLR VDAVASMLYR 

       430        440        450        460        470        480 
DYSRKEGEWL PNRHGGRENL EAIDFLHHLN QVVASETPGA LVIAEESTAW PGVSRPVAEG 

       490        500        510        520        530        540 
GLGFSHKWNM GWMHDSLAYI GEDPLHRRYH HHQLTFGLLY AFSEHFILPI SHDEVVHGKH 

       550        560        570        580        590        600 
SLLDKMPGDR WQKFANLRLY LSFMWSHPGK KLLFMGCEFG QWREWNHDGE LDWYLLRYPE 

       610        620        630        640        650        660 
HQGVQDLVAA LNRLYRELPA LHARDGEALG FEWLIGDDQA NSVYAWLRHA AGEPSLLAVH 

       670        680        690        700        710        720 
NFTPVPRQGY RIGVPQGGDW DVLLNSDAQA FAGSGAGSQG RVSSESCGAH GQAQSLVLDL 

       730 
PPLGTLLLRP AG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004091 Genomic DNA. Translation: AAG05541.1.
PIRH83376.
RefSeqNP_250843.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9I1W2.
SMRQ9I1W2. Positions 123-728.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA2153.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG05541; AAG05541; PA2153.
GeneID881350.
KEGGpae:PA2153.
PATRIC19838744. VBIPseAer58763_2252.

Organism-specific databases

PseudoCAPPA2153.

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAISEGRHE.
OrthoDBEOG6JX7GT.
PhylomeDBQ9I1W2.

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_PSEAE
AccessionPrimary (citable) accession number: Q9I1W2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries