Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei412 – 4121NucleophileUniRule annotation
Active sitei465 – 4651Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:PA2153
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA2153.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7327321,4-alpha-glucan branching enzyme GlgBPRO_0000188730Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA2153.

Structurei

3D structure databases

ProteinModelPortaliQ9I1W2.
SMRiQ9I1W2. Positions 38-106, 123-728.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
InParanoidiQ9I1W2.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.
PhylomeDBiQ9I1W2.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I1W2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPMSERER WQLELDKLQR GLQGDPFAFL GPQRDPGGEG GVLRAYLPGA
60 70 80 90 100
QRVELLDEDG ATLAELEQSD PGSGLFQRHL ERLPPRYRLR VHWPDGVQES
110 120 130 140 150
EDPYAFGPLL GELDLYLFAE GNHRQLASCL GAQLTRHEGV EGVRFAVWAP
160 170 180 190 200
NAVRVSVVGD FNGWDGRRHP MRRRYPSGVW ELFVPRLGEG ELYKYELQGH
210 220 230 240 250
DGLLPLKADP LALACETPPG TASKTCAALR HEWRDQDWLA RRAERQGYAA
260 270 280 290 300
PLSIYEVHAG SWRHRDGRPP HWSELAEELI PYVRQLGFTH IELMPVMEHP
310 320 330 340 350
FGGSWGYQPL GLFAPTARYG TPEDFAAFVD ACHQAGIGVI LDWVPAHFPT
360 370 380 390 400
DAHGLGRFDG TALYEYEHPF EGFHQDWDTY IYNLGRSEVH GFMLASALHW
410 420 430 440 450
LRTYHVDGLR VDAVASMLYR DYSRKEGEWL PNRHGGRENL EAIDFLHHLN
460 470 480 490 500
QVVASETPGA LVIAEESTAW PGVSRPVAEG GLGFSHKWNM GWMHDSLAYI
510 520 530 540 550
GEDPLHRRYH HHQLTFGLLY AFSEHFILPI SHDEVVHGKH SLLDKMPGDR
560 570 580 590 600
WQKFANLRLY LSFMWSHPGK KLLFMGCEFG QWREWNHDGE LDWYLLRYPE
610 620 630 640 650
HQGVQDLVAA LNRLYRELPA LHARDGEALG FEWLIGDDQA NSVYAWLRHA
660 670 680 690 700
AGEPSLLAVH NFTPVPRQGY RIGVPQGGDW DVLLNSDAQA FAGSGAGSQG
710 720 730
RVSSESCGAH GQAQSLVLDL PPLGTLLLRP AG
Length:732
Mass (Da):82,563
Last modified:March 1, 2001 - v1
Checksum:iC31303D6D5F929F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG05541.1.
PIRiH83376.
RefSeqiNP_250843.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG05541; AAG05541; PA2153.
GeneIDi881350.
KEGGipae:PA2153.
PATRICi19838744. VBIPseAer58763_2252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG05541.1.
PIRiH83376.
RefSeqiNP_250843.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ9I1W2.
SMRiQ9I1W2. Positions 38-106, 123-728.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA2153.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG05541; AAG05541; PA2153.
GeneIDi881350.
KEGGipae:PA2153.
PATRICi19838744. VBIPseAer58763_2252.

Organism-specific databases

PseudoCAPiPA2153.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
InParanoidiQ9I1W2.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.
PhylomeDBiQ9I1W2.

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiGLGB_PSEAE
AccessioniPrimary (citable) accession number: Q9I1W2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.