Reviewed, UniProtKB/Swiss-Prot Q9I1M2 (ODBA_PSEAE)

Names and origin

Protein names

Recommended name:
    2-oxoisovalerate dehydrogenase subunit alpha
    EC=1.2.4.4
Alternative name(s):
    Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
      Short name=BCKDH E1-alpha

Gene names

Name:	bkdA1
Ordered Locus Names:	PA2247

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2.
Cofactor	Thiamine pyrophosphate By similarity.
Subunit structure	Heterodimer of an alpha and a beta chain By similarity.
Sequence similarities	Belongs to the BCKDHA family.

Ontologies

Keywords
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	2-oxoisovalerate dehydrogenase subunit alpha		PRO_0000287782

Sequences

	Sequence		Length	Mass (Da)
	Q9I1M2-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: BE3AF6FAB66F0F01	FASTA	410	45,256
	10 20 30 40 50 60 MSDYEPLRLH VPEPTGRPGC KTDFSYLHLS PAGEVRKPPV DVEPAETSDL AYSLVRVLDD 70 80 90 100 110 120 DGHAVGPWNP QLSNEQLLRG MRAMLKTRLF DARMLTAQRQ KKLSFYMQCL GEEAIATAHT 130 140 150 160 170 180 LALRDGDMCF PTYRQQGILI TREYPLVDMI CQLLSNEADP LKGRQLPIMY SSKEAGFFSI 190 200 210 220 230 240 SGNLATQFIQ AVGWGMASAI KGDTRIASAW IGDGATAESD FHTALTFAHV YRAPVILNVV 250 260 270 280 290 300 NNQWAISTFQ AIAGGEGTTF ANRGVGCGIA SLRVDGNDFL AVYAASEWAA ERARRNLGPS 310 320 330 340 350 360 LIEWVTYRAG PHSTSDDPSK YRPADDWTNF PLGDPIARLK RHMIGLGIWS EEQHEATHKA 370 380 390 400 410 LEAEVLAAQK QAESHGTLID GRVPSAASMF EDVYAELPEH LRRQRQELGV

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG05635.1.
PIR	C83365. S05057.
RefSeq	NP_250937.1.
3D structure databases
HSSP	HSSP built from PDB template 1QS0 based on UniProtKB P09060.
SMR	Q9I1M2. Positions 2-408.
ModBase	Search...
Genome annotation databases
GeneID	877901.
GenomeReviews	Gene locus PA2247 in contig AE004091_GR.
KEGG	pae:PA2247.
Organism-specific databases
PseudoCAP	PA2247.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I1M2.
OMA	MQSLGEE.
Enzyme and pathway databases
BioCyc	PAER208964:PA2247-MON.
BRENDA	1.2.4.4. 354.
Family and domain databases
InterPro	IPR001017. DH_E1. [Graphical view]
Pfam	PF00676. E1_dh. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ODBA_PSEAE

Accession

Primary (citable) accession number: Q9I1M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)