Reviewed, UniProtKB/Swiss-Prot Q9I1C2 (MSUD_PSEAE)

Names and origin

Protein names

Recommended name:
    Methanesulfonate monooxygenase
    EC=1.14.14.5
Alternative name(s):
    Methanesulfonate sulfonatase
    FMNH2-dependent methanesulfonate monooxygenase

Gene names

Name:	msuD
Ordered Locus Names:	PA2356

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the desulfonation of aliphatic sulfonates. Shows highest activity with methanesulfonate. Ref.1
Catalytic activity	An alkanesufonate (R-CH(2)-SO3H) + FMNH2 + O2 = an aldehyde (R-CHO) + FMN + sulfite + H2O. HAMAP MF_01229
Induction	Repressed by sulfate, cysteine, or thiocyanate. HAMAP MF_01229
Miscellaneous	FMNH2 which is absolutely required for this enzymatic reaction, is provided by msuE. HAMAP MF_01229
Sequence similarities	Belongs to the ssuD family.

Ontologies

Keywords
Ligand	FMN
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanesulfonate monooxygenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Methanesulfonate monooxygenase HAMAP MF_01229		PRO_0000216710
Experimental info
	Sequence conflict	28	1	P → S in AAB82564. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9I1C2-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F39FF1C2E3217747	FASTA	381	41,596
	10 20 30 40 50 60 MNVFWFLPTH GDGHFLGTSQ GARPVSLPYL KQVAQAADSL GYHGVLIPTG RSCEDSWVVA 70 80 90 100 110 120 SALAPLTERL RFLVAIRPGI VSPTVSARMA ATLDRLSGGR LLINVVTGGD PDENRGDGIH 130 140 150 160 170 180 LGHAERYEVT DEFLRVWRRV LQGEAVDFHG KHIHVENAKA LYPPLQRPYP PLYFGGSSEA 190 200 210 220 230 240 AHELAGEQVD VYLTWGEPLP AVAAKIADVR QRAARHGRTV KFGIRLHVIV RETAEEAWRA 250 260 270 280 290 300 ADRLIEHISD ETIAAAQQSF ARFDSEGQRR MAALHGGRRD RLEIQPNLWA GVGLVRGGAG 310 320 330 340 350 360 TALVGDPRQV AERIGEYAEL GIDSFIFSGY PHLEEAYRFA ELVFPLLPEP YASLAGRGLT 370 380 NLTGPFGEMI ANDVLPARAG A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel reduced flavin mononucleotide-dependent methanesulfonate sulfonatase encoded by the sulfur-regulated msu operon of Pseudomonas aeruginosa." Kertesz M.A., Schmidt-Larbig K., Wueest T. J. Bacteriol. 181:1464-1473(1999) [PubMed: 10049377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF026067 Genomic DNA. Translation: AAB82564.1. AE004091 Genomic DNA. Translation: AAG05744.1.
PIR	E83352.
RefSeq	NP_251046.1.
3D structure databases
HSSP	HSSP built from PDB template 1M41 based on UniProtKB P80645.
SMR	Q9I1C2. Positions 1-347.
ModBase	Search...
Genome annotation databases
GeneID	879651.
GenomeReviews	Gene locus PA2356 in contig AE004091_GR.
KEGG	pae:PA2356.
Organism-specific databases
PseudoCAP	PA2356.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I1C2.
OMA	Q9I1C2. GGDPVEN.
Enzyme and pathway databases
BioCyc	PAER208964:PA2356-MON.
BRENDA	1.14.14.5. 354.
Family and domain databases
HAMAP	MF_01229. [Tree]
InterPro	IPR019911. Alkanesulfonate_mOase_FMN-dep. IPR011251. Luciferase-like. IPR016048. Luciferase-like_sub. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03565. Alk_sulf_monoox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSUD_PSEAE

Accession

Primary (citable) accession number: Q9I1C2
Secondary accession number(s): O31039

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2003
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)