Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q9I1C2 (MSUD_PSEAE)

Last modified June 16, 2009. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Methanesulfonate monooxygenase
    EC=1.14.14.5
Alternative name(s):
    Methanesulfonate sulfonatase
    FMNH2-dependent methanesulfonate monooxygenase
Gene names
Name: msuD
Ordered Locus Names: PA2356
OrganismPseudomonas aeruginosa [Complete proteome] [HAMAP]
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Catalyzes the desulfonation of aliphatic sulfonates. Shows highest activity with methanesulfonate. Ref.1

Catalytic activity

An alkanesufonate (R-CH(2)-SO3H) + FMNH2 + O2 = an aldehyde (R-CHO) + FMN + sulfite + H2O. HAMAP MF_01229

Induction

Repressed by sulfate, cysteine, or thiocyanate. HAMAP MF_01229

Miscellaneous

FMNH2 which is absolutely required for this enzymatic reaction, is provided by msuE. HAMAP MF_01229

Sequence similarities

Belongs to the ssuD family.

Ontologies

Keywords
   LigandFMN
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionalkanesulfonate monooxygenase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 381381Methanesulfonate monooxygenase HAMAP MF_01229
PRO_0000216710

Experimental info

Sequence conflict281P → S in AAB82564. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9I1C2-1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: F39FF1C2E3217747

FASTA38141,596
        10         20         30         40         50         60 
MNVFWFLPTH GDGHFLGTSQ GARPVSLPYL KQVAQAADSL GYHGVLIPTG RSCEDSWVVA 

        70         80         90        100        110        120 
SALAPLTERL RFLVAIRPGI VSPTVSARMA ATLDRLSGGR LLINVVTGGD PDENRGDGIH 

       130        140        150        160        170        180 
LGHAERYEVT DEFLRVWRRV LQGEAVDFHG KHIHVENAKA LYPPLQRPYP PLYFGGSSEA 

       190        200        210        220        230        240 
AHELAGEQVD VYLTWGEPLP AVAAKIADVR QRAARHGRTV KFGIRLHVIV RETAEEAWRA 

       250        260        270        280        290        300 
ADRLIEHISD ETIAAAQQSF ARFDSEGQRR MAALHGGRRD RLEIQPNLWA GVGLVRGGAG 

       310        320        330        340        350        360 
TALVGDPRQV AERIGEYAEL GIDSFIFSGY PHLEEAYRFA ELVFPLLPEP YASLAGRGLT 

       370        380 
NLTGPFGEMI ANDVLPARAG A 

« Hide

References

« Hide 'large scale' references
[1]"A novel reduced flavin mononucleotide-dependent methanesulfonate sulfonatase encoded by the sulfur-regulated msu operon of Pseudomonas aeruginosa."
Kertesz M.A., Schmidt-Larbig K., Wueest T.
J. Bacteriol. 181:1464-1473(1999) [PubMed: 10049377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed: 10984043] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases

AF026067 Genomic DNA. Translation: AAB82564.1.
AE004091 Genomic DNA. Translation: AAG05744.1.
PIRE83352.
RefSeqNP_251046.1.

3D structure databases

HSSPHSSP built from PDB template 1M41 based on UniProtKB P80645.
SMRQ9I1C2. Positions 1-347.
ModBaseSearch...

Genome annotation databases

GeneID879651.
GenomeReviewsGene locus PA2356 in contig AE004091_GR.
KEGGpae:PA2356.

Organism-specific databases

PseudoCAPPA2356.
CMRSearch...

Phylogenomic databases

HOGENOMQ9I1C2.
OMAQ9I1C2. GGDPVEN.

Enzyme and pathway databases

BioCycPAER208964:PA2356-MON.
BRENDA1.14.14.5. 354.

Family and domain databases

HAMAPMF_01229.
[Tree]
InterProIPR019911. Alkanesulfonate_mOase_FMN-dep.
IPR011251. Luciferase-like.
IPR016048. Luciferase-like_sub.
[Graphical view]
Gene3DG3DSA:3.20.20.30. Luciferase_like. 1 hit.
PfamPF00296. Bac_luciferase. 1 hit.
[Graphical view]
TIGRFAMsTIGR03565. Alk_sulf_monoox. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMSUD_PSEAE
AccessionPrimary (citable) accession number: Q9I1C2
Secondary accession number(s): O31039
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: March 1, 2001
Last modified: June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents