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Q9I138 (GLYA2_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase 2
Short name=SHMT 2
Short name=Serine methylase 2
EC=2.1.2.1
Gene names
Name:glyA2
Ordered Locus Names:PA2444
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 418418Pyridoxal-phosphate-dependent serine hydroxymethyltransferase 2
PRO_0000113638

Regions

Region125 – 1273Substrate binding By similarity

Sites

Binding site351Pyridoxal phosphate By similarity
Binding site551Pyridoxal phosphate By similarity
Binding site571Substrate By similarity
Binding site641Substrate binding By similarity
Binding site651Pyridoxal phosphate By similarity
Binding site991Pyridoxal phosphate By similarity
Binding site1211Substrate By similarity
Binding site1761Pyridoxal phosphate By similarity
Binding site2041Pyridoxal phosphate By similarity
Binding site2291Pyridoxal phosphate By similarity
Binding site2361Pyridoxal phosphate By similarity
Binding site2631Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3631Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2301N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9I138 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: A94D7ABF6A58D9D4

FASTA41844,974
        10         20         30         40         50         60 
MFSKHDQLQG YDDELLAAMD AEDRRQEDHI ELIASENYAS KRVMQAQGGG LTNKYAEGYP 

        70         80         90        100        110        120 
GKRYYGGCEH VDKVERLAID RARQLFGADY ANVQPHSGSS ANAAVYLALL NAGDTILGMS 

       130        140        150        160        170        180 
LAHGGHLTHG AKVSSSGKLY NAVQYGLDTA TGLIDYDEVE RLAVEHKPKM IVAGFSAYSK 

       190        200        210        220        230        240 
TLDFPRFRAI ADKVGALLFV DMAHVAGLVA AGLYPNPIPF ADVVTTTTHK TLRGPRGGLI 

       250        260        270        280        290        300 
LARANEEIEK KLNSAVFPGA QGGPLMHVIA AKAVCFKEAL EPGFKDYQAQ VIRNAKAMAE 

       310        320        330        340        350        360 
VFIGRGYDVV SGGTDNHLML ISLVKQGLTG KAADAALGAA HITVNKNAVP NDPQSPFVTS 

       370        380        390        400        410 
GIRIGTPAVT TRGFREGECR ELAGWICDIL DDIDNPEVGE RVRGQVGEFC RHFPVYAD

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE004091 Genomic DNA. Translation: AAG05832.1.
PIRC83341.
RefSeqNP_251134.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9I138.
SMRQ9I138. Positions 1-417.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID882925.
GenomeReviewsGene locus PA2444 in contig AE004091_GR.
KEGGpae:PA2444.
NMPDRfig|208964.1.peg.2444.
PATRIC19839353. VBIPseAer58763_2556.

Organism-specific databases

PseudoCAPPA2444.

Phylogenomic databases

HOGENOMHBG301263.
OMALELCSIA.
ProtClustDBPRK13034.

Enzyme and pathway databases

BioCycPAER208964:PA2444-MONOMER.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA2_PSEAE
AccessionPrimary (citable) accession number: Q9I138
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 1, 2001
Last modified: January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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