Q9I0Q7 (T23O_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	PA2579

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Tryptophan 2,3-dioxygenase		PRO_0000360124
Regions
	Region	32 – 36	5	Substrate binding By similarity
	Region	57 – 61	5	Substrate binding By similarity
Sites
	Metal binding	246	1	Iron (heme axial ligand) By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	130	1	Heme By similarity
	Binding site	260	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I0Q7 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2D394B81528A0E05	FASTA	288	32,932
	10 20 30 40 50 60 MCPCPHSQAQ GETDGEAEWH NAALDFTHAM SYGDYLKLDK VLDAQFPLSP DHNEMLFIIQ 70 80 90 100 110 120 HQTSELWMKL MLHELRAARE HVKSGKLGPA LKMLARVSRI FDQLVHAWAV LATMTPTEYN 130 140 150 160 170 180 TIRPYLGQSS GFQSYQYREI EFILGNKNAT LLKPHAHRAE LLAALEQALH TPSLYDEAIR 190 200 210 220 230 240 LMAAQGLPVS QERLARDAAA GTCYEASVEA AWRQVYQAPE RYWDLYQLAE KLIDLEDSFR 250 260 270 280 QWRFRHVTTV ERIIGFKPGT GGTEGVGYLR SMLDTILFPE LWRLRSNL

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG05967.1.
PIR	E83323.
RefSeq	NP_251269.1. NC_002516.2.
3D structure databases
HSSP	HSSP built from PDB template 1YW0 based on UniProtKB Q8PDA8.
ProteinModelPortal	Q9I0Q7.
SMR	Q9I0Q7. Positions 30-288.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	879140.
GenomeReviews	Gene locus PA2579 in contig AE004091_GR.
KEGG	pae:PA2579.
PATRIC	19839641. VBIPseAer58763_2699.
Organism-specific databases
PseudoCAP	PA2579.
Phylogenomic databases
HOGENOM	HBG647485.
OMA	QYREIEF.
ProtClustDB	CLSK867383.
Enzyme and pathway databases
BioCyc	PAER208964:PA2579-MONOMER.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_PSEAE

Accession

Primary (citable) accession number: Q9I0Q7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program