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Protein

DNA translocase FtsK

Gene

ftsK

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif (Probable).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei675 – 6751ATP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi469 – 4746ATP
Nucleotide bindingi693 – 6942ATP

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cellular response to antibiotic Source: PseudoCAP
  • chromosome segregation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA translocase FtsK
Gene namesi
Name:ftsK
Ordered Locus Names:PA2615
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA2615.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei31 – 5323HelicalSequence analysisAdd
BLAST
Transmembranei73 – 9523HelicalSequence analysisAdd
BLAST
Transmembranei116 – 13823HelicalSequence analysisAdd
BLAST
Transmembranei148 – 16518HelicalSequence analysisAdd
BLAST
Transmembranei172 – 19423HelicalSequence analysisAdd
BLAST
Topological domaini195 – 811617CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 811811DNA translocase FtsKPRO_0000098278Add
BLAST

Proteomic databases

PaxDbiQ9I0M3.

Interactioni

Subunit structurei

Homohexamer. Forms a ring that surrounds DNA.2 Publications

Protein-protein interaction databases

STRINGi208964.PA2615.

Structurei

Secondary structure

1
811
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi318 – 3203Combined sources
Helixi333 – 34917Combined sources
Beta strandi356 – 3616Combined sources
Beta strandi363 – 3708Combined sources
Helixi378 – 3836Combined sources
Helixi385 – 3928Combined sources
Beta strandi398 – 4014Combined sources
Beta strandi409 – 4146Combined sources
Helixi423 – 4275Combined sources
Helixi430 – 4334Combined sources
Beta strandi438 – 4469Combined sources
Beta strandi451 – 4555Combined sources
Helixi456 – 4583Combined sources
Beta strandi462 – 4654Combined sources
Helixi472 – 48413Combined sources
Turni489 – 4913Combined sources
Beta strandi492 – 4976Combined sources
Beta strandi499 – 5013Combined sources
Helixi502 – 5054Combined sources
Turni506 – 5094Combined sources
Beta strandi513 – 5164Combined sources
Helixi521 – 54525Combined sources
Helixi550 – 56213Combined sources
Beta strandi575 – 5784Combined sources
Beta strandi589 – 5957Combined sources
Helixi600 – 6034Combined sources
Helixi606 – 61813Combined sources
Turni620 – 6234Combined sources
Beta strandi624 – 6318Combined sources
Turni635 – 6373Combined sources
Helixi640 – 6445Combined sources
Beta strandi648 – 6525Combined sources
Helixi657 – 6648Combined sources
Beta strandi665 – 6673Combined sources
Helixi669 – 6713Combined sources
Beta strandi677 – 6815Combined sources
Beta strandi683 – 6864Combined sources
Beta strandi689 – 6935Combined sources
Helixi698 – 70912Combined sources
Turni718 – 7214Combined sources
Beta strandi742 – 7465Combined sources
Helixi750 – 76011Combined sources
Helixi765 – 7728Combined sources
Helixi776 – 78813Combined sources
Beta strandi791 – 7933Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IUTX-ray2.25A/B247-811[»]
2IUUX-ray2.90A/B/C/D/E/F247-728[»]
2J5ONMR-A742-811[»]
2VE8X-ray1.40A/B/C/D/E/F/G/H739-811[»]
2VE9X-ray1.90A/B/C/D/E/F739-811[»]
ProteinModelPortaliQ9I0M3.
SMRiQ9I0M3. Positions 315-722, 744-811.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9I0M3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini449 – 661213FtsKPROSITE-ProRule annotationAdd
BLAST

Domaini

Consists of an N-terminal domain, which is sufficient for the localization to the septal ring and is required for cell division, followed by a linker domain, and a C-terminal domain, which forms the translocation motor involved in chromosome segregation. The C-terminal domain can be further subdivided into alpha, beta and gamma subdomains. The alpha and beta subdomains multimerise to produce a hexameric ring, contain the nucleotide binding motif and form the DNA pump. The gamma subdomain is a regulatory subdomain that controls translocation of DNA by recognition of KOPS motifs and interacts with XerD recombinase (Probable).1 Publication

Sequence similaritiesi

Belongs to the FtsK/SpoIIIE/SftA family.Curated
Contains 1 FtsK domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CNU. Bacteria.
COG1674. LUCA.
HOGENOMiHOG000010003.
InParanoidiQ9I0M3.
KOiK03466.
OMAiYFAYIFP.
OrthoDBiEOG6S52GD.
PhylomeDBiQ9I0M3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR025199. FtsK_4TM.
IPR002543. FtsK_dom.
IPR018541. Ftsk_gamma.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13491. FtsK_4TM. 1 hit.
PF09397. Ftsk_gamma. 1 hit.
PF01580. FtsK_SpoIIIE. 1 hit.
[Graphical view]
SMARTiSM00843. Ftsk_gamma. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50901. FTSK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I0M3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRKNSDLKD STTASHAAAW RQQLHSRLKE GVLIALGALC LYLWMALLTY
60 70 80 90 100
DSADPSWSHS SQVDQVQNAA GRLGAVSADI LFMTLGYFAY LFPLLLGIKT
110 120 130 140 150
WQVFRRRNLP WEWNTWLFSW RLVGLIFLIL AGSALAYIHF HASGHMPASA
160 170 180 190 200
SAGGAIGQSL GRVAVDALNV QGSTLVFFAL FLFGLTVFAD LSWFKVMDVT
210 220 230 240 250
GKITLDFFEL IQNAFNRWMG ARAERKQLVA QLREVDERVA EVVAPSVPDR
260 270 280 290 300
REQSKAKERL LEREEALAKH MSEREKRPPP KIDPPPSPKA PEPSKRVLKE
310 320 330 340 350
KQAPLFVDTA VEGTLPPLSL LDPAEVKQKS YSPESLEAMS RLLEIKLKEF
360 370 380 390 400
GVEVSVDSVH PGPVITRFEI QPAAGVKVSR ISNLAKDLAR SLAVISVRVV
410 420 430 440 450
EVIPGKTTVG IEIPNEDRQM VRFSEVLSSP EYDEHKSTVP LALGHDIGGR
460 470 480 490 500
PIITDLAKMP HLLVAGTTGS GKSVGVNAML LSILFKSTPS EARLIMIDPK
510 520 530 540 550
MLELSIYEGI PHLLCPVVTD MKEAANALRW SVAEMERRYR LMAAMGVRNL
560 570 580 590 600
AGFNRKVKDA EEAGTPLTDP LFRRESPDDE PPQLSTLPTI VVVVDEFADM
610 620 630 640 650
MMIVGKKVEE LIARIAQKAR AAGIHLILAT QRPSVDVITG LIKANIPTRI
660 670 680 690 700
AFQVSSKIDS RTILDQGGAE QLLGHGDMLY LPPGTGLPIR VHGAFVSDDE
710 720 730 740 750
VHRVVEAWKL RGAPDYIEDI LAGVDEGGGG GGSFDGGDGS GEGSEDDPLY
760 770 780 790 800
DEAVRFVTES RRASISAVQR KLKIGYNRAA RMIEAMEMAG VVTPMNTNGS
810
REVIAPAPVR D
Length:811
Mass (Da):88,894
Last modified:March 1, 2001 - v1
Checksum:i3174CA63E8190405
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06003.1.
PIRiE83318.
RefSeqiNP_251305.1. NC_002516.2.
WP_010895627.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06003; AAG06003; PA2615.
GeneIDi882321.
KEGGipae:PA2615.
PATRICi19839721. VBIPseAer58763_2736.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06003.1.
PIRiE83318.
RefSeqiNP_251305.1. NC_002516.2.
WP_010895627.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IUTX-ray2.25A/B247-811[»]
2IUUX-ray2.90A/B/C/D/E/F247-728[»]
2J5ONMR-A742-811[»]
2VE8X-ray1.40A/B/C/D/E/F/G/H739-811[»]
2VE9X-ray1.90A/B/C/D/E/F739-811[»]
ProteinModelPortaliQ9I0M3.
SMRiQ9I0M3. Positions 315-722, 744-811.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA2615.

Proteomic databases

PaxDbiQ9I0M3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06003; AAG06003; PA2615.
GeneIDi882321.
KEGGipae:PA2615.
PATRICi19839721. VBIPseAer58763_2736.

Organism-specific databases

PseudoCAPiPA2615.

Phylogenomic databases

eggNOGiENOG4105CNU. Bacteria.
COG1674. LUCA.
HOGENOMiHOG000010003.
InParanoidiQ9I0M3.
KOiK03466.
OMAiYFAYIFP.
OrthoDBiEOG6S52GD.
PhylomeDBiQ9I0M3.

Miscellaneous databases

EvolutionaryTraceiQ9I0M3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR025199. FtsK_4TM.
IPR002543. FtsK_dom.
IPR018541. Ftsk_gamma.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13491. FtsK_4TM. 1 hit.
PF09397. Ftsk_gamma. 1 hit.
PF01580. FtsK_SpoIIIE. 1 hit.
[Graphical view]
SMARTiSM00843. Ftsk_gamma. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50901. FTSK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. "Double-stranded DNA translocation: structure and mechanism of hexameric FtsK."
    Massey T.H., Mercogliano C.P., Yates J., Sherratt D.J., Lowe J.
    Mol. Cell 23:457-469(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 247-811 IN COMPLEX WITH ADP, FUNCTION AS A TRANSLOCASE, SUBUNIT.
  3. Cited for: STRUCTURE BY NMR OF 739-811, DNA-BINDING, DOMAIN.
  4. "Molecular mechanism of sequence-directed DNA loading and translocation by FtsK."
    Lowe J., Ellonen A., Allen M.D., Atkinson C., Sherratt D.J., Grainge I.
    Mol. Cell 31:498-509(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 739-811, FUNCTION, SUBUNIT, DNA-BINDING.

Entry informationi

Entry nameiFTSK_PSEAE
AccessioniPrimary (citable) accession number: Q9I0M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: March 1, 2001
Last modified: December 9, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.