Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA translocase FtsK

Gene

ftsK

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif (Probable).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei675ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi469 – 474ATP6
Nucleotide bindingi693 – 694ATP2

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cellular response to antibiotic Source: PseudoCAP
  • chromosome segregation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA translocase FtsK
Gene namesi
Name:ftsK
Ordered Locus Names:PA2615
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA2615.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei31 – 53HelicalSequence analysisAdd BLAST23
Transmembranei73 – 95HelicalSequence analysisAdd BLAST23
Transmembranei116 – 138HelicalSequence analysisAdd BLAST23
Transmembranei148 – 165HelicalSequence analysisAdd BLAST18
Transmembranei172 – 194HelicalSequence analysisAdd BLAST23
Topological domaini195 – 811CytoplasmicSequence analysisAdd BLAST617

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000982781 – 811DNA translocase FtsKAdd BLAST811

Proteomic databases

PaxDbiQ9I0M3.

Interactioni

Subunit structurei

Homohexamer. Forms a ring that surrounds DNA.2 Publications

Protein-protein interaction databases

STRINGi208964.PA2615.

Structurei

Secondary structure

1811
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi318 – 320Combined sources3
Helixi333 – 349Combined sources17
Beta strandi356 – 361Combined sources6
Beta strandi363 – 370Combined sources8
Helixi378 – 383Combined sources6
Helixi385 – 392Combined sources8
Beta strandi398 – 401Combined sources4
Beta strandi409 – 414Combined sources6
Helixi423 – 427Combined sources5
Helixi430 – 433Combined sources4
Beta strandi438 – 446Combined sources9
Beta strandi451 – 455Combined sources5
Helixi456 – 458Combined sources3
Beta strandi462 – 465Combined sources4
Helixi472 – 484Combined sources13
Turni489 – 491Combined sources3
Beta strandi492 – 497Combined sources6
Beta strandi499 – 501Combined sources3
Helixi502 – 505Combined sources4
Turni506 – 509Combined sources4
Beta strandi513 – 516Combined sources4
Helixi521 – 545Combined sources25
Helixi550 – 562Combined sources13
Beta strandi575 – 578Combined sources4
Beta strandi589 – 595Combined sources7
Helixi600 – 603Combined sources4
Helixi606 – 618Combined sources13
Turni620 – 623Combined sources4
Beta strandi624 – 631Combined sources8
Turni635 – 637Combined sources3
Helixi640 – 644Combined sources5
Beta strandi648 – 652Combined sources5
Helixi657 – 664Combined sources8
Beta strandi665 – 667Combined sources3
Helixi669 – 671Combined sources3
Beta strandi677 – 681Combined sources5
Beta strandi683 – 686Combined sources4
Beta strandi689 – 693Combined sources5
Helixi698 – 709Combined sources12
Turni718 – 721Combined sources4
Beta strandi742 – 746Combined sources5
Helixi750 – 760Combined sources11
Helixi765 – 772Combined sources8
Helixi776 – 788Combined sources13
Beta strandi791 – 793Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IUTX-ray2.25A/B247-811[»]
2IUUX-ray2.90A/B/C/D/E/F247-728[»]
2J5ONMR-A742-811[»]
2VE8X-ray1.40A/B/C/D/E/F/G/H739-811[»]
2VE9X-ray1.90A/B/C/D/E/F739-811[»]
ProteinModelPortaliQ9I0M3.
SMRiQ9I0M3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9I0M3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini449 – 661FtsKPROSITE-ProRule annotationAdd BLAST213

Domaini

Consists of an N-terminal domain, which is sufficient for the localization to the septal ring and is required for cell division, followed by a linker domain, and a C-terminal domain, which forms the translocation motor involved in chromosome segregation. The C-terminal domain can be further subdivided into alpha, beta and gamma subdomains. The alpha and beta subdomains multimerise to produce a hexameric ring, contain the nucleotide binding motif and form the DNA pump. The gamma subdomain is a regulatory subdomain that controls translocation of DNA by recognition of KOPS motifs and interacts with XerD recombinase (Probable).1 Publication

Sequence similaritiesi

Belongs to the FtsK/SpoIIIE/SftA family.Curated
Contains 1 FtsK domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CNU. Bacteria.
COG1674. LUCA.
HOGENOMiHOG000010003.
InParanoidiQ9I0M3.
KOiK03466.
OMAiYFAYIFP.
PhylomeDBiQ9I0M3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR025199. FtsK_4TM.
IPR002543. FtsK_dom.
IPR018541. Ftsk_gamma.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13491. FtsK_4TM. 1 hit.
PF09397. Ftsk_gamma. 1 hit.
PF01580. FtsK_SpoIIIE. 1 hit.
[Graphical view]
SMARTiSM00843. Ftsk_gamma. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50901. FTSK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I0M3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRKNSDLKD STTASHAAAW RQQLHSRLKE GVLIALGALC LYLWMALLTY
60 70 80 90 100
DSADPSWSHS SQVDQVQNAA GRLGAVSADI LFMTLGYFAY LFPLLLGIKT
110 120 130 140 150
WQVFRRRNLP WEWNTWLFSW RLVGLIFLIL AGSALAYIHF HASGHMPASA
160 170 180 190 200
SAGGAIGQSL GRVAVDALNV QGSTLVFFAL FLFGLTVFAD LSWFKVMDVT
210 220 230 240 250
GKITLDFFEL IQNAFNRWMG ARAERKQLVA QLREVDERVA EVVAPSVPDR
260 270 280 290 300
REQSKAKERL LEREEALAKH MSEREKRPPP KIDPPPSPKA PEPSKRVLKE
310 320 330 340 350
KQAPLFVDTA VEGTLPPLSL LDPAEVKQKS YSPESLEAMS RLLEIKLKEF
360 370 380 390 400
GVEVSVDSVH PGPVITRFEI QPAAGVKVSR ISNLAKDLAR SLAVISVRVV
410 420 430 440 450
EVIPGKTTVG IEIPNEDRQM VRFSEVLSSP EYDEHKSTVP LALGHDIGGR
460 470 480 490 500
PIITDLAKMP HLLVAGTTGS GKSVGVNAML LSILFKSTPS EARLIMIDPK
510 520 530 540 550
MLELSIYEGI PHLLCPVVTD MKEAANALRW SVAEMERRYR LMAAMGVRNL
560 570 580 590 600
AGFNRKVKDA EEAGTPLTDP LFRRESPDDE PPQLSTLPTI VVVVDEFADM
610 620 630 640 650
MMIVGKKVEE LIARIAQKAR AAGIHLILAT QRPSVDVITG LIKANIPTRI
660 670 680 690 700
AFQVSSKIDS RTILDQGGAE QLLGHGDMLY LPPGTGLPIR VHGAFVSDDE
710 720 730 740 750
VHRVVEAWKL RGAPDYIEDI LAGVDEGGGG GGSFDGGDGS GEGSEDDPLY
760 770 780 790 800
DEAVRFVTES RRASISAVQR KLKIGYNRAA RMIEAMEMAG VVTPMNTNGS
810
REVIAPAPVR D
Length:811
Mass (Da):88,894
Last modified:March 1, 2001 - v1
Checksum:i3174CA63E8190405
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06003.1.
PIRiE83318.
RefSeqiNP_251305.1. NC_002516.2.
WP_010895627.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06003; AAG06003; PA2615.
GeneIDi882321.
KEGGipae:PA2615.
PATRICi19839721. VBIPseAer58763_2736.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06003.1.
PIRiE83318.
RefSeqiNP_251305.1. NC_002516.2.
WP_010895627.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IUTX-ray2.25A/B247-811[»]
2IUUX-ray2.90A/B/C/D/E/F247-728[»]
2J5ONMR-A742-811[»]
2VE8X-ray1.40A/B/C/D/E/F/G/H739-811[»]
2VE9X-ray1.90A/B/C/D/E/F739-811[»]
ProteinModelPortaliQ9I0M3.
SMRiQ9I0M3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA2615.

Proteomic databases

PaxDbiQ9I0M3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06003; AAG06003; PA2615.
GeneIDi882321.
KEGGipae:PA2615.
PATRICi19839721. VBIPseAer58763_2736.

Organism-specific databases

PseudoCAPiPA2615.

Phylogenomic databases

eggNOGiENOG4105CNU. Bacteria.
COG1674. LUCA.
HOGENOMiHOG000010003.
InParanoidiQ9I0M3.
KOiK03466.
OMAiYFAYIFP.
PhylomeDBiQ9I0M3.

Miscellaneous databases

EvolutionaryTraceiQ9I0M3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR025199. FtsK_4TM.
IPR002543. FtsK_dom.
IPR018541. Ftsk_gamma.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF13491. FtsK_4TM. 1 hit.
PF09397. Ftsk_gamma. 1 hit.
PF01580. FtsK_SpoIIIE. 1 hit.
[Graphical view]
SMARTiSM00843. Ftsk_gamma. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50901. FTSK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFTSK_PSEAE
AccessioniPrimary (citable) accession number: Q9I0M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.