PA2634

Functionⁱ

Involved in the metabolic adaptation in response to environmental changes. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates.By similarity

Catalytic activityⁱ

Isocitrate = succinate + glyoxylate.By similarity

Cofactorⁱ

Mg²⁺By similarity

Pathwayⁱ: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

Isocitrate lyase (PA2634)
Malate synthase G (glcB)

This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	184	MagnesiumBy similarity	1
Active siteⁱ	222	Proton acceptorBy similarity	1
Binding siteⁱ	451	SubstrateBy similarity	1

GO - Molecular functionⁱ

isocitrate lyase activity
Source: PseudoCAP
Inferred from direct assayⁱ
- 17319879
metal ion binding Source: UniProtKB-KW

Complete GO annotation...

GO - Biological processⁱ

glyoxylate cycle Source: UniProtKB-UniPathway
negative regulation of single-species biofilm formation on inanimate substrate
Source: PseudoCAP
Inferred from mutant phenotypeⁱ
- 23363478
pathogenesis
Source: PseudoCAP
Inferred from mutant phenotypeⁱ
- 18524916
tricarboxylic acid cycle Source: UniProtKB-KW

Complete GO annotation...

Keywordsⁱ

Molecular function	Lyase
Biological process	Glyoxylate bypass, Tricarboxylic acid cycle
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAⁱ	4.1.3.1. 5087.
UniPathwayⁱ	UPA00703; UER00719.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity) Short name: ICLBy similarity Alternative name(s): IsocitraseBy similarity IsocitrataseBy similarity
Gene namesⁱ	Ordered Locus Names:PA2634
Organismⁱ	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifierⁱ	208964 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa
Proteomesⁱ	UP000002438 Componentⁱ: Chromosome

Organism-specific databases

Pseudomonas genome database More...PseudoCAPⁱ	PA2634.

PseudoCAPⁱ

PA2634.

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000068780	1 – 531	Isocitrate lyaseAdd BLAST		531

Proteomic databases

PaxDbⁱ	Q9I0K4.
PRIDEⁱ	Q9I0K4.

Interactionⁱ

Subunit structureⁱ

Homotetramer.By similarity

Protein-protein interaction databases

STRINGⁱ	208964.PA2634.

STRINGⁱ

208964.PA2634.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9I0K4.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	101 – 103	Substrate bindingBy similarity	3
Regionⁱ	223 – 224	Substrate bindingBy similarity	2
Regionⁱ	380 – 384	Substrate bindingBy similarity	5

Sequence similaritiesⁱ

Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4107RET. Bacteria. COG2224. LUCA.
HOGENOMⁱ	HOG000191811.
InParanoidⁱ	Q9I0K4.
KEGG Orthology (KO) More...KOⁱ	K01637.
OMAⁱ	MHDRKQW.
PhylomeDBⁱ	Q9I0K4.

Family and domain databases

InterProⁱ	View protein in InterPro IPR006254. Isocitrate_lyase. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
PANTHERⁱ	PTHR21631. PTHR21631. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00463. ICL. 3 hits.
PIRSFⁱ	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAMⁱ	SSF51621. SSF51621. 2 hits.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9I0K4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSAYQNEIKA VAALKEKNGS SWSAINPEYA ARMRIQNRFK TGLDIAKYTA 
        60         70         80         90        100
AIMRKDMAEY DADSSVYTQS LGCWHGFIGQ QKLISIKKHL KTTNKRYLYL 
       110        120        130        140        150
SGWMVAALRS DFGPLPDQSM HEKTAVSGLI EELYTFLRQA DARELDLLFT 
       160        170        180        190        200
GLDAARAAGD KAKEAELLAQ IDNFETHVVP IIADIDAGFG NAEATYLLAK 
       210        220        230        240        250
KMIEAGACCI QIENQVSDEK QCGHQDGKVT VPHIDFLAKI NAVRYAFLEL 
       260        270        280        290        300
GVDDGVIVAR TDSLGAGLTK QIAVTNEPGD LGDLYNSFLD CEEISESELG 
       310        320        330        340        350
NGDVVIKREG KLLRPKRLAS NLFQFRKGTG EDRCVLDCIT SLQNGADLLW 
       360        370        380        390        400
IETEKPHVGQ IKAMVDRIRE VIPNAKLVYN NSPSFNWTLN FRQQVFDAFV 
       410        420        430        440        450
AEGKDVSAYD RNKLMSVEYD DTELAKVADE KIRTFQRDGS AHAGIFHHLI 
       460        470        480        490        500
TLPTYHTAAL STDNLAKGYF ADEGMLAYVK GVQRQELRQG IACVKHQNMA 
       510        520        530 
GSDIGDNHKE YFAGEAALKA SGKDNTMNQF H

Length:531

Mass (Da):58,887

Last modified:March 1, 2001 - v1

Checksum:ⁱF45A4ECDA3F220CA

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE004091 Genomic DNA. Translation: AAG06022.1.
PIRⁱ	G83315.
NCBI Reference Sequences More...RefSeqⁱ	NP_251324.1. NC_002516.2. WP_003113373.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaⁱ	AAG06022; AAG06022; PA2634.
GeneIDⁱ	882341.
KEGGⁱ	pae:PA2634.
PATRICⁱ	fig\|208964.12.peg.2756.

Similar proteinsⁱ

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9I0K4	A0A0E1AS62 UPI000281BFEF A0A1G5KVW1 A0A1E9D4P8 A0A212EAU1 A0A1Y6MZA5 A0A0P1DAN1 A0A157W8Y4 A0A1W5Y6Z4 A0A072ZPH9 +195	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) Pseudomonas aeruginosa MTB-1 Pseudomonas aeruginosa Acinetobacter baumannii Pseudomonas sp. HMSC066B11 Pseudomonas aeruginosa 148 Pseudomonas aeruginosa C-NN2 Pseudomonas aeruginosa DK1 Enterobacter cloacae Pseudomonas aeruginosa (strain PAK) And more	531	UniRef90_Q9I0K4	Cluster: Isocitrate lyase	206

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9I0K4	O50078 UPI0003B66ED7 A0A037UTK1 A0A167B9C5 A0A0P1IX01 A0A154C5E4 A0A1V0FMU9 K0CDG3 UPI0003E7D72F A0A095UGZ2 +882	Hyphomicrobium methylovorum Spongiibacter tropicus Rhodomicrobium udaipurense JA643 Alcanivorax sp. HI0044 Alcanivorax dieselolei Alcanivorax sp. KX64203 Alcanivorax xenomutans Alcanivorax dieselolei (strain DSM 16502 / CGMCC 1.3690 / B-5) Alcanivorax Alcanivorax jadensis T9 And more	540	UniRef50_O50078	Cluster: Isocitrate lyase	893

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE004091 Genomic DNA. Translation: AAG06022.1.
PIRⁱ	G83315.
NCBI Reference Sequences More...RefSeqⁱ	NP_251324.1. NC_002516.2. WP_003113373.1. NC_002516.2.

3D structure databases

ProteinModelPortalⁱ	Q9I0K4.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	208964.PA2634.

STRINGⁱ

208964.PA2634.

Proteomic databases

PaxDbⁱ	Q9I0K4.
PRIDEⁱ	Q9I0K4.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAG06022; AAG06022; PA2634.
GeneIDⁱ	882341.
KEGGⁱ	pae:PA2634.
PATRICⁱ	fig\|208964.12.peg.2756.

Organism-specific databases

Pseudomonas genome database More...PseudoCAPⁱ	PA2634.

PseudoCAPⁱ

PA2634.

Phylogenomic databases

eggNOGⁱ	ENOG4107RET. Bacteria. COG2224. LUCA.
HOGENOMⁱ	HOG000191811.
InParanoidⁱ	Q9I0K4.
KEGG Orthology (KO) More...KOⁱ	K01637.
OMAⁱ	MHDRKQW.
PhylomeDBⁱ	Q9I0K4.

Enzyme and pathway databases

UniPathwayⁱ	UPA00703; UER00719.
BRENDAⁱ	4.1.3.1. 5087.

Family and domain databases

InterProⁱ	View protein in InterPro IPR006254. Isocitrate_lyase. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
PANTHERⁱ	PTHR21631. PTHR21631. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00463. ICL. 3 hits.
PIRSFⁱ	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAMⁱ	SSF51621. SSF51621. 2 hits.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ACEA_PSEAE
Accessionⁱ	Q9I0K4Primary (citable) accession number: Q9I0K4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
	Last sequence update:	March 1, 2001
	Last modified:	August 30, 2017
	This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB

UniParc

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Annotation systems

Supporting data

UniProtKB - Q9I0K4 (ACEA_PSEAE)

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Isocitrate lyase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glyoxylate cycle

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Pathwayⁱ: glyoxylate cycle

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ