Q9I0K4 (ACEA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate lyase
Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1

Gene names

Ordered Locus Names:

PA2634

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway By similarity.
Catalytic activity	Isocitrate = succinate + glyoxylate. UniProtKB P28298
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway negative regulation of single-species biofilm formation on inanimate substrate Inferred from mutant phenotype PubMed 23363478. Source: PseudoCAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from direct assay PubMed 17319879. Source: PseudoCAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 531	531	Isocitrate lyase		PRO_0000068780
Sites
	Active site	222	1	By similarity UniProtKB P28298

Sequences

	Sequence		Length	Mass (Da)
	Q9I0K4 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F45A4ECDA3F220CA	FASTA	531	58,887
	10 20 30 40 50 60 MSAYQNEIKA VAALKEKNGS SWSAINPEYA ARMRIQNRFK TGLDIAKYTA AIMRKDMAEY 70 80 90 100 110 120 DADSSVYTQS LGCWHGFIGQ QKLISIKKHL KTTNKRYLYL SGWMVAALRS DFGPLPDQSM 130 140 150 160 170 180 HEKTAVSGLI EELYTFLRQA DARELDLLFT GLDAARAAGD KAKEAELLAQ IDNFETHVVP 190 200 210 220 230 240 IIADIDAGFG NAEATYLLAK KMIEAGACCI QIENQVSDEK QCGHQDGKVT VPHIDFLAKI 250 260 270 280 290 300 NAVRYAFLEL GVDDGVIVAR TDSLGAGLTK QIAVTNEPGD LGDLYNSFLD CEEISESELG 310 320 330 340 350 360 NGDVVIKREG KLLRPKRLAS NLFQFRKGTG EDRCVLDCIT SLQNGADLLW IETEKPHVGQ 370 380 390 400 410 420 IKAMVDRIRE VIPNAKLVYN NSPSFNWTLN FRQQVFDAFV AEGKDVSAYD RNKLMSVEYD 430 440 450 460 470 480 DTELAKVADE KIRTFQRDGS AHAGIFHHLI TLPTYHTAAL STDNLAKGYF ADEGMLAYVK 490 500 510 520 530 GVQRQELRQG IACVKHQNMA GSDIGDNHKE YFAGEAALKA SGKDNTMNQF H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Biofouling in water treatment systems: effect of membrane properties on biofilm formation." Liddor M. Thesis (2005), Ben-Gurion University, Israel Cited for: PROTEIN SEQUENCE OF 110-138; 144-156; 245-260; 318-325; 393-404; 414-426 AND 468-480. Strain: ATCC 33467 / type 1 smooth.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG06022.1.
PIR	G83315.
RefSeq	NP_251324.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I0K4.
SMR	Q9I0K4. Positions 79-506.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	208964.PA2634.
Proteomic databases
PRIDE	Q9I0K4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG06022; AAG06022; PA2634.
GeneID	882341.
KEGG	pae:PA2634.
PATRIC	19839761. VBIPseAer58763_2756.
Organism-specific databases
PseudoCAP	PA2634.
Phylogenomic databases
eggNOG	COG2224.
HOGENOM	HOG000191811.
KO	K01637.
OMA	VDEIAGM.
OrthoDB	EOG689HMX.
PhylomeDB	Q9I0K4.
Enzyme and pathway databases
BRENDA	4.1.3.1. 5087.
UniPathway	UPA00703; UER00719.
Family and domain databases
Gene3D	3.20.20.60. 3 hits.
InterPro	IPR006254. Isocitrate_lyase. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 3 hits. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. SSF51621. 2 hits.
ProtoNet	Search...

Entry information

Entry name

ACEA_PSEAE

Accession

Primary (citable) accession number: Q9I0K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	March 1, 2001
Last modified:	July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program