PA2634

Functionⁱ

Involved in the metabolic adaptation in response to environmental changes. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates.By similarity

Catalytic activityⁱ

Isocitrate = succinate + glyoxylate.By similarity

Cofactorⁱ

Mg²⁺By similarity

Pathwayⁱ: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

Isocitrate lyase (PA2634)
Malate synthase G (glcB)

This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	184	MagnesiumBy similarity	1
Active siteⁱ	222	Proton acceptorBy similarity	1
Binding siteⁱ	451	SubstrateBy similarity	1

GO - Molecular functionⁱ

isocitrate lyase activity
Source: PseudoCAP
Inferred from direct assayⁱ
- 17319879
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

glyoxylate cycle Source: UniProtKB-UniPathway
negative regulation of single-species biofilm formation on inanimate substrate
Source: PseudoCAP
Inferred from mutant phenotypeⁱ
- 23363478
pathogenesis
Source: PseudoCAP
Inferred from mutant phenotypeⁱ
- 18524916
tricarboxylic acid cycle Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Lyase
Biological process	Glyoxylate bypass, Tricarboxylic acid cycle
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

BioCycⁱ	PAER208964:G1FZ6-2674-MONOMER
BRENDAⁱ	4.1.3.1 5087
UniPathwayⁱ	UPA00703; UER00719

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity) Short name: ICLBy similarity Alternative name(s): IsocitraseBy similarity IsocitrataseBy similarity
Gene namesⁱ	Ordered Locus Names:PA2634
Organismⁱ	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifierⁱ	208964 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa
Proteomesⁱ	UP000002438 Componentⁱ: Chromosome

Organism-specific databases

Pseudomonas genome database More...PseudoCAPⁱ	PA2634

PseudoCAPⁱ

PA2634

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000068780	1 – 531	Isocitrate lyaseAdd BLAST		531

Proteomic databases

PaxDbⁱ	Q9I0K4
PRIDEⁱ	Q9I0K4

Interactionⁱ

Subunit structureⁱ

Homotetramer.By similarity

Protein-protein interaction databases

STRINGⁱ	208964.PA2634

STRINGⁱ

208964.PA2634

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9I0K4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	101 – 103	Substrate bindingBy similarity	3
Regionⁱ	223 – 224	Substrate bindingBy similarity	2
Regionⁱ	380 – 384	Substrate bindingBy similarity	5

Sequence similaritiesⁱ

Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4107RET Bacteria COG2224 LUCA
HOGENOMⁱ	HOG000191811
InParanoidⁱ	Q9I0K4
KEGG Orthology (KO) More...KOⁱ	K01637
OMAⁱ	MHDRKQW
PhylomeDBⁱ	Q9I0K4

Family and domain databases

InterProⁱ	View protein in InterPro IPR006254 Isocitrate_lyase IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERⁱ	PTHR21631 PTHR21631, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00463 ICL, 3 hits
PIRSFⁱ	PIRSF001362 Isocit_lyase, 1 hit
SUPFAMⁱ	SSF51621 SSF51621, 2 hits

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9I0K4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSAYQNEIKA VAALKEKNGS SWSAINPEYA ARMRIQNRFK TGLDIAKYTA 
        60         70         80         90        100
AIMRKDMAEY DADSSVYTQS LGCWHGFIGQ QKLISIKKHL KTTNKRYLYL 
       110        120        130        140        150
SGWMVAALRS DFGPLPDQSM HEKTAVSGLI EELYTFLRQA DARELDLLFT 
       160        170        180        190        200
GLDAARAAGD KAKEAELLAQ IDNFETHVVP IIADIDAGFG NAEATYLLAK 
       210        220        230        240        250
KMIEAGACCI QIENQVSDEK QCGHQDGKVT VPHIDFLAKI NAVRYAFLEL 
       260        270        280        290        300
GVDDGVIVAR TDSLGAGLTK QIAVTNEPGD LGDLYNSFLD CEEISESELG 
       310        320        330        340        350
NGDVVIKREG KLLRPKRLAS NLFQFRKGTG EDRCVLDCIT SLQNGADLLW 
       360        370        380        390        400
IETEKPHVGQ IKAMVDRIRE VIPNAKLVYN NSPSFNWTLN FRQQVFDAFV 
       410        420        430        440        450
AEGKDVSAYD RNKLMSVEYD DTELAKVADE KIRTFQRDGS AHAGIFHHLI 
       460        470        480        490        500
TLPTYHTAAL STDNLAKGYF ADEGMLAYVK GVQRQELRQG IACVKHQNMA 
       510        520        530 
GSDIGDNHKE YFAGEAALKA SGKDNTMNQF H

Length:531

Mass (Da):58,887

Last modified:March 1, 2001 - v1

Checksum:ⁱF45A4ECDA3F220CA

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE004091 Genomic DNA Translation: AAG06022.1
PIRⁱ	G83315
NCBI Reference Sequences More...RefSeqⁱ	NP_251324.1, NC_002516.2 WP_003113373.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaⁱ	AAG06022; AAG06022; PA2634
GeneIDⁱ	882341
KEGGⁱ	pae:PA2634
PATRICⁱ	fig\|208964.12.peg.2756

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9I0K4	A0A0E1AS62 A0A1G5KVW1 A0A157W8Y4 A0A1E9D4P8 A0A072ZPH9 A0A1S1CA30 W1MHX5 A0A0H2ZAX9 V6ADR4 A0A0H3R9T0 +233	Pseudomonas aeruginosa MTB-1 Acinetobacter baumannii Enterobacter cloacae Pseudomonas sp. HMSC066B11 Pseudomonas aeruginosa Pseudomonas sp. HMSC059F05 Pseudomonas aeruginosa VRFPA03 Pseudomonas aeruginosa (strain UCBPP-PA14) Pseudomonas aeruginosa MH27 Pseudomonas aeruginosa str. Stone 130 And more	531	UniRef90_Q9I0K4	Cluster: Isocitrate lyase	244

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9I0K4	A0A0E1AS62 A0A1G5KVW1 A0A157W8Y4 A0A1E9D4P8 A0A072ZPH9 A0A1S1CA30 W1MHX5 A0A0H2ZAX9 V6ADR4 A0A0H3R9T0 +380	Pseudomonas aeruginosa MTB-1 Acinetobacter baumannii Enterobacter cloacae Pseudomonas sp. HMSC066B11 Pseudomonas aeruginosa Pseudomonas sp. HMSC059F05 Pseudomonas aeruginosa VRFPA03 Pseudomonas aeruginosa (strain UCBPP-PA14) Pseudomonas aeruginosa MH27 Pseudomonas aeruginosa str. Stone 130 And more	531	UniRef50_Q9I0K4	Cluster: Isocitrate lyase	391

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE004091 Genomic DNA Translation: AAG06022.1
PIRⁱ	G83315
NCBI Reference Sequences More...RefSeqⁱ	NP_251324.1, NC_002516.2 WP_003113373.1, NC_002516.2

3D structure databases

ProteinModelPortalⁱ	Q9I0K4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	208964.PA2634

STRINGⁱ

208964.PA2634

Proteomic databases

PaxDbⁱ	Q9I0K4
PRIDEⁱ	Q9I0K4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAG06022; AAG06022; PA2634
GeneIDⁱ	882341
KEGGⁱ	pae:PA2634
PATRICⁱ	fig\|208964.12.peg.2756

Organism-specific databases

Pseudomonas genome database More...PseudoCAPⁱ	PA2634

PseudoCAPⁱ

PA2634

Phylogenomic databases

eggNOGⁱ	ENOG4107RET Bacteria COG2224 LUCA
HOGENOMⁱ	HOG000191811
InParanoidⁱ	Q9I0K4
KEGG Orthology (KO) More...KOⁱ	K01637
OMAⁱ	MHDRKQW
PhylomeDBⁱ	Q9I0K4

Enzyme and pathway databases

UniPathwayⁱ	UPA00703; UER00719
BioCycⁱ	PAER208964:G1FZ6-2674-MONOMER
BRENDAⁱ	4.1.3.1 5087

Family and domain databases

InterProⁱ	View protein in InterPro IPR006254 Isocitrate_lyase IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERⁱ	PTHR21631 PTHR21631, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00463 ICL, 3 hits
PIRSFⁱ	PIRSF001362 Isocit_lyase, 1 hit
SUPFAMⁱ	SSF51621 SSF51621, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ACEA_PSEAE
Accessionⁱ	Q9I0K4Primary (citable) accession number: Q9I0K4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
	Last sequence update:	March 1, 2001
	Last modified:	February 28, 2018
	This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9I0K4 (ACEA_PSEAE)

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Isocitrate lyase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glyoxylate cycle

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: glyoxylate cycle

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ