Reviewed, UniProtKB/Swiss-Prot Q9I0K1 (NUOA2_PSEAE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit A 2
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit A 2
    NDH-1 subunit A 2
    NUO1 2

Gene names

Name:	nuoA2
Ordered Locus Names:	PA2637

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity. HAMAP MF_01394
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 137	137	NADH-quinone oxidoreductase subunit A 2 HAMAP MF_01394		PRO_0000287834
Regions
	Transmembrane	12 – 32	21	Potential
	Transmembrane	66 – 86	21	Potential
	Transmembrane	95 – 115	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9I0K1-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: AACE0C233E45AA83	FASTA	137	15,010
	10 20 30 40 50 60 MPNPAELAAH HWGFAAFLLG VVGLLAFMLG VSSLLGSKAF GRSKNEPFES GIVPTGGARL 70 80 90 100 110 120 RLSAKFYLVA MLFVIFDVEA LFLFAWSVSV RESGWAGLIE ATIFIAILLA GLVYLWRIGA 130 LDWAPESRRK RQAKLKQ

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG06025.1.
PIR	B83316.
RefSeq	NP_251327.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	882344.
GenomeReviews	Gene locus PA2637 in contig AE004091_GR.
KEGG	pae:PA2637.
Organism-specific databases
PseudoCAP	PA2637.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG605540.
OMA	FIEATIF.
Enzyme and pathway databases
BioCyc	PAER208964:PA2637-MONOMER.
BRENDA	1.6.99.5. 354.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA2_PSEAE

Accession

Primary (citable) accession number: Q9I0K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)