Reviewed, UniProtKB/Swiss-Prot Q9I0J9 (NUOCD_PSEAE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit C/D
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit C/D
    NDH-1 subunit C/D

Gene names

Name:	nuoC
Synonyms:	nuoCD, nuoD
Ordered Locus Names:	PA2639

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01359
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits nuoB, CD, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	In the N-terminal section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	NADH-quinone oxidoreductase subunit C/D HAMAP MF_01359		PRO_0000287861
Regions
	Region	1 – 184	184	NADH dehydrogenase I subunit C By similarity
	Region	208 – 593	386	NADH dehydrogenase I subunit D By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I0J9-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: DC3C824522843DC6	FASTA	593	68,323
	10 20 30 40 50 60 MTADSALYIP PYKADDQDIV VELNSRFGAE TFTVQPTRTG MPVLWVPRER LIEVLTFLRQ 70 80 90 100 110 120 VPKPYVMLYD LHGVDERLRT HRRGLPSADF SVFYHLMSLE RNSDVMIKVA LSERDLNLPT 130 140 150 160 170 180 ATRIWPNANW YEREVWDMYG ITFTGHPHLT RMLMPPTWQG HPLRKDYPAR ATEFDPYSLS 190 200 210 220 230 240 AAKQDLEQEA LRFKPEDWGM KRHGENEDYM FLNLGPNHPS AHGAFRIILQ LDGEEIIDCV 250 260 270 280 290 300 PEIGYHHRGA EKMAERQSWH SFIPYTDRID YLGGVMNNLP YVLSVEKLAG IKVPQRVDVI 310 320 330 340 350 360 RIMMAEFFRI LNHLLYLGTY IQDVGAMTPV FFTFTDRQRA YKVVEAITGF RLHPAWYRIG 370 380 390 400 410 420 GVAHDLPRGW DKLVREFLDW MPKRLDEYET AALKNSILRG RTIGVAQYNT KEALEWGTTG 430 440 450 460 470 480 AGLRATGCDF DLRKARPYSG YENFEFEVPL AHNGDAYDRC MVKMGEMRQS LRIIEQCLKN 490 500 510 520 530 540 MPEGPYKADH PLTTPPPKER TLQHIETLIT HFLQVSWGPV MPANEAFQMI EATKGINSYY 550 560 570 580 590 LTSDGSTMSY RTRIRTPSFA HLQQIPSVIN GSMIADLIAY LGSIDFVMAD VDR

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG06027.1.
PIR	D83316.
RefSeq	NP_251329.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	882346.
GenomeReviews	Gene locus PA2639 in contig AE004091_GR.
KEGG	pae:PA2639.
Organism-specific databases
PseudoCAP	PA2639.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I0J9.
OMA	Q9I0J9. PPTWEGH.
Enzyme and pathway databases
BioCyc	PAER208964:PA2639-MON.
BRENDA	1.6.99.5. 354.
Family and domain databases
HAMAP	MF_01359. [Tree]
InterPro	IPR010219. NADH_DH_1_dsu. IPR010218. NADH_DH_csu. IPR001135. NADH_Q_OxRdtase_suD. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_su-49kDa_CS. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. False negative. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOCD_PSEAE

Accession

Primary (citable) accession number: Q9I0J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)