Reviewed, UniProtKB/Swiss-Prot Q9I0J7 (NUOF_PSEAE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit F
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit F
    NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	PA2641

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Subunit structure	Composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex By similarity.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	NADH-quinone oxidoreductase subunit F		PRO_0000287849
Regions
	Nucleotide binding	69 – 78	10	NAD By similarity
	Nucleotide binding	182 – 229	48	FMN By similarity
Sites
	Metal binding	361	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	364	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	367	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	408	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9I0J7-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 6820CA50DA9A656A	FASTA	448	48,679
	10 20 30 40 50 60 MTLTSIGPAN RMARSAETHP LTWRLREDAQ PVWLEEYQSK DGYAAARKAL TQMAQDDIVQ 70 80 90 100 110 120 TVKDSGLKGR GGAGFPTGVK WGLMPKDESL NIRYLLCNAD EMEPNTWKDR MLMEQLPHLL 130 140 150 160 170 180 VEGMLISARA LKAYRGYIFL RGEYVDAARN LNRAIDEAKA AGLLGKNILG SGFDFELFVH 190 200 210 220 230 240 TGAGRYICGE ETALINSLEG RRANPRSKPP FPAAVGVWGK PTCVNNVETL CNVPAIVGNG 250 260 270 280 290 300 VDWYKTLARP GSEDMGTKLM GFSGKVKNPG LWELPFGVSA RELFEDYAGG MRDGFQLKAW 310 320 330 340 350 360 QPGGAGTGFL LPEHLDAQMF AGGIAKVGTR MGTGLAMAVD DSINMVSLLR NMEEFFARES 370 380 390 400 410 420 CGWCTPCRDG LPWSVKLLRA LERGEGQPGD LETLEQLVNF LGPGKTFCAH APGAVEPLGS 430 440 ALKYFRAEFE AGISRQPSAA PRPATVGA

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG06029.1.
PIR	F83316.
RefSeq	NP_251331.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	882348.
GenomeReviews	Gene locus PA2641 in contig AE004091_GR.
KEGG	pae:PA2641.
NMPDR	fig|208964.1.peg.2641.
Organism-specific databases
PseudoCAP	PA2641.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I0J7.
OMA	Q9I0J7. LPHLLIE.
Enzyme and pathway databases
BioCyc	PAER208964:PA2641-MON.
BRENDA	1.6.99.5. 354.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51KDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51KDa_su. IPR019554. Soluble_ligand_bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_PSEAE

Accession

Primary (citable) accession number: Q9I0J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)