Reviewed, UniProtKB/Swiss-Prot Q9I0J6 (NUOG_PSEAE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit G
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit G
    NDH-1 subunit G

Gene names

Name:	nuoG
Ordered Locus Names:	PA2642

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 3 4Fe-4S clusters per subunit By similarity.
Subunit structure	Composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 905	905	NADH-quinone oxidoreductase subunit G		PRO_0000118549
Regions
	Domain	1 – 83	83	2Fe-2S ferredoxin-type
Sites
	Metal binding	34	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	45	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	48	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	67	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	99	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	103	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	106	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	112	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	151	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	154	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	157	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	201	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	228	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	231	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	235	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	263	1	Iron-sulfur 4 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9I0J6-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 775E4CD402A8F311	FASTA	905	99,000
	10 20 30 40 50 60 MATIHVDGKT LEVDGADNLL QACLSLGLDI PYFCWHPALG SVGACRQCAV KQYTDENDKR 70 80 90 100 110 120 GRLVMSCMTP ATDNTWISIE DEEAKQFRAS VVEWLMTNHP HDCPVCEEGG HCHLQDMTVM 130 140 150 160 170 180 TGHNERRYRF TKRTHQNQEL GPFIAHEMNR CIACYRCVRY YKDYAGGTDL GVYGAHDNVY 190 200 210 220 230 240 FGRVEDGVLE SEFSGNLTEV CPTGVFTDKT HSERYNRKWD MQFAPSICHG CSSGCNISPG 250 260 270 280 290 300 ERYGEIRRIE NRYNGSVNHY FLCDRGRFGY GYVNREDRPR QPLLVLSKQK LSLDGALDQA 310 320 330 340 350 360 AALLKERKVV GIGSPRASLE SNFALRELVG EGNFYSGINE GELDRLRLIL QVMQEGPLPV 370 380 390 400 410 420 PSIRDIEDHD AVFVLGEDLT QTAARIALAL RQSVKGKAVE MAADMKVQPW LDAAVKNIAQ 430 440 450 460 470 480 HAQNPLFIAS VSATRLDDVA EETVHAAPDD LARLGFAVAH AIDPSAPSVA DLDPQAQAFA 490 500 510 520 530 540 QRITDALLAA KRPLVVSGNS LGNKALIEAA ANIAKALKQR EKNGSISLVV GEANSLGLAL 550 560 570 580 590 600 FGGDSVEAAL ERLTSGQADA VVVLENDLYR RTDAARVDAA LAAAKVVIVA DHQQTATTAK 610 620 630 640 650 660 AHLVLPAASF AEGDGTLVSQ EGRAQRFFQV FDPTYYDARN MVREGWRWLH AIHSTLQGKR 670 680 690 700 710 720 VDWTQLDHVT EAVAEAKPIL AGIRDAAPAA SFRIKGLKLA REPHRYSGRT AMRANISVHE 730 740 750 760 770 780 PRTPQDIDSA FAFSMEGYSG SQEDRQQIPF AWSPGWNSPQ AWNKFQDEVG GHLRAGDPGV 790 800 810 820 830 840 RLIEPKGEGL DWFQAVPVPF SAKADSWKVV PLYHLFGSEE NSSRAAPIQQ RIPETYVALS 850 860 870 880 890 900 KEDADRLGVN DGATLGFQLK GQALRLPLRI DEQLGAGLIG LPVGFAGIPA AIAGCSVEGL QEAAQ

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG06030.1.
PIR	G83314.
RefSeq	NP_251332.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	882349.
GenomeReviews	Gene locus PA2642 in contig AE004091_GR.
KEGG	pae:PA2642.
NMPDR	fig|208964.1.peg.2642.
Organism-specific databases
PseudoCAP	PA2642.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I0J6.
OMA	Q9I0J6. EVGGHLR.
Enzyme and pathway databases
BioCyc	PAER208964:PA2642-MON.
BRENDA	1.6.99.5. 354.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR000283. NADH_UbQ_OxRdtase_75KDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF00111. Fer2. 1 hit. PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOG_PSEAE

Accession

Primary (citable) accession number: Q9I0J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)