NADH-quinone oxidoreductase subunit G - Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Preferred order of sections

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit G
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit G
NDH-1 subunit G

Gene names

Name:	nuoG
Ordered Locus Names:	PA2642

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD⁺ + quinol.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 3 4Fe-4S clusters per subunit By similarity.
Subunit structure	Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 905

905

NADH-quinone oxidoreductase subunit G

PRO_0000118549

Regions

Domain

1 – 83

83

2Fe-2S ferredoxin-type

Sites

Metal binding

34

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

45

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

48

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

67

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

99

1

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

103

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

106

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

112

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

151

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

154

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

157

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

201

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

228

1

Iron-sulfur 4 (4Fe-4S) Potential

Metal binding

231

1

Iron-sulfur 4 (4Fe-4S) Potential

Metal binding

235

1

Iron-sulfur 4 (4Fe-4S) Potential

Metal binding

263

1

Iron-sulfur 4 (4Fe-4S) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q9I0J6 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 775E4CD402A8F311
Show »

FASTA

905

99,000

        10         20         30         40         50         60 
MATIHVDGKT LEVDGADNLL QACLSLGLDI PYFCWHPALG SVGACRQCAV KQYTDENDKR 

        70         80         90        100        110        120 
GRLVMSCMTP ATDNTWISIE DEEAKQFRAS VVEWLMTNHP HDCPVCEEGG HCHLQDMTVM 

       130        140        150        160        170        180 
TGHNERRYRF TKRTHQNQEL GPFIAHEMNR CIACYRCVRY YKDYAGGTDL GVYGAHDNVY 

       190        200        210        220        230        240 
FGRVEDGVLE SEFSGNLTEV CPTGVFTDKT HSERYNRKWD MQFAPSICHG CSSGCNISPG 

       250        260        270        280        290        300 
ERYGEIRRIE NRYNGSVNHY FLCDRGRFGY GYVNREDRPR QPLLVLSKQK LSLDGALDQA 

       310        320        330        340        350        360 
AALLKERKVV GIGSPRASLE SNFALRELVG EGNFYSGINE GELDRLRLIL QVMQEGPLPV 

       370        380        390        400        410        420 
PSIRDIEDHD AVFVLGEDLT QTAARIALAL RQSVKGKAVE MAADMKVQPW LDAAVKNIAQ 

       430        440        450        460        470        480 
HAQNPLFIAS VSATRLDDVA EETVHAAPDD LARLGFAVAH AIDPSAPSVA DLDPQAQAFA 

       490        500        510        520        530        540 
QRITDALLAA KRPLVVSGNS LGNKALIEAA ANIAKALKQR EKNGSISLVV GEANSLGLAL 

       550        560        570        580        590        600 
FGGDSVEAAL ERLTSGQADA VVVLENDLYR RTDAARVDAA LAAAKVVIVA DHQQTATTAK 

       610        620        630        640        650        660 
AHLVLPAASF AEGDGTLVSQ EGRAQRFFQV FDPTYYDARN MVREGWRWLH AIHSTLQGKR 

       670        680        690        700        710        720 
VDWTQLDHVT EAVAEAKPIL AGIRDAAPAA SFRIKGLKLA REPHRYSGRT AMRANISVHE 

       730        740        750        760        770        780 
PRTPQDIDSA FAFSMEGYSG SQEDRQQIPF AWSPGWNSPQ AWNKFQDEVG GHLRAGDPGV 

       790        800        810        820        830        840 
RLIEPKGEGL DWFQAVPVPF SAKADSWKVV PLYHLFGSEE NSSRAAPIQQ RIPETYVALS 

       850        860        870        880        890        900 
KEDADRLGVN DGATLGFQLK GQALRLPLRI DEQLGAGLIG LPVGFAGIPA AIAGCSVEGL 


QEAAQ

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References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R.

Olson M.V.
Nature 406:959-964(2000) [PubMed: 10984043] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG06030.1.
PIR	G83314.
RefSeq	NP_251332.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I0J6.
ModBase	Search...
Proteomic databases
PRIDE	Q9I0J6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	882349.
GenomeReviews	Gene locus PA2642 in contig AE004091_GR.
KEGG	pae:PA2642.
NMPDR	fig\|208964.1.peg.2642.
PATRIC	19839779. VBIPseAer58763_2765.
Organism-specific databases
PseudoCAP	PA2642.
Phylogenomic databases
HOGENOM	HBG488172.
OMA	PSICHGC.
ProtClustDB	PRK08166.
Enzyme and pathway databases
BioCyc	PAER208964:PA2642-MONOMER.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR012675. Beta-grasp_ferredoxin-type. IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K00336.
Pfam	PF00111. Fer2. 1 hit. PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. SM00929. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit. SSF54292. Ferredoxin. 1 hit.
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOG_PSEAE

Accession

Primary (citable) accession number: Q9I0J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents