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Protein

Phenylalanine--tRNA ligase alpha subunit

Gene

pheS

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per tetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi252 – 2521MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase alpha subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunitUniRule annotation
Short name:
PheRSUniRule annotation
Gene namesi
Name:pheSUniRule annotation
Ordered Locus Names:PA2740
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA2740.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Phenylalanine--tRNA ligase alpha subunitPRO_0000126744Add
BLAST

Proteomic databases

PaxDbiQ9I0A3.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA2740.

Structurei

Secondary structure

1
338
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi108 – 12114Combined sources
Turni122 – 1243Combined sources
Beta strandi132 – 1354Combined sources
Helixi136 – 1394Combined sources
Helixi141 – 1433Combined sources
Helixi150 – 1523Combined sources
Turni154 – 1563Combined sources
Beta strandi159 – 1668Combined sources
Beta strandi168 – 1703Combined sources
Helixi171 – 18010Combined sources
Beta strandi185 – 19410Combined sources
Beta strandi199 – 2013Combined sources
Beta strandi204 – 21714Combined sources
Helixi220 – 23415Combined sources
Beta strandi241 – 2488Combined sources
Beta strandi251 – 26010Combined sources
Beta strandi277 – 28610Combined sources
Helixi288 – 2936Combined sources
Turni298 – 3003Combined sources
Beta strandi302 – 3098Combined sources
Helixi310 – 3189Combined sources
Helixi325 – 3284Combined sources
Helixi331 – 3344Combined sources
Helixi335 – 3373Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P71X-ray2.79C/D1-338[»]
4P72X-ray2.62C/D1-338[»]
4P73X-ray3.03C/D1-338[»]
4P74X-ray2.70C/D1-338[»]
4P75X-ray2.96C/D1-338[»]
ProteinModelPortaliQ9I0A3.
SMRiQ9I0A3. Positions 7-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CSS. Bacteria.
COG0016. LUCA.
HOGENOMiHOG000242675.
InParanoidiQ9I0A3.
KOiK01889.
OMAiEYHPARD.
PhylomeDBiQ9I0A3.

Family and domain databases

HAMAPiMF_00281. Phe_tRNA_synth_alpha1. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR004188. Phe-tRNA_ligase_II_N.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR010978. tRNA-bd_arm.
[Graphical view]
PfamiPF02912. Phe_tRNA-synt_N. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I0A3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENLDALVSQ ALEAVRHTED VNALEQIRVH YLGKKGELTQ VMKTLGDLPA
60 70 80 90 100
EERPKVGALI NVAKEKVQDV LNARKTELEG AALAARLAAE RIDVTLPGRG
110 120 130 140 150
QLSGGLHPVT RTLERIEQCF SRIGYEVAEG PEVEDDYHNF EALNIPGHHP
160 170 180 190 200
ARAMHDTFYF NANMLLRTHT SPVQVRTMES QQPPIRIVCP GRVYRCDSDL
210 220 230 240 250
THSPMFHQVE GLLVDEGVSF ADLKGTIEEF LRAFFEKQLE VRFRPSFFPF
260 270 280 290 300
TEPSAEVDIQ CVICSGNGCR VCKQTGWLEV MGCGMVHPNV LRMSNIDPEK
310 320 330
FQGFAFGMGA ERLAMLRYGV NDLRLFFDND LRFLGQFR
Length:338
Mass (Da):38,064
Last modified:March 1, 2001 - v1
Checksum:i6FEA3219E322F0FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06128.1.
PIRiB83303.
RefSeqiNP_251430.1. NC_002516.2.
WP_003114409.1. NZ_ASJY01000445.1.

Genome annotation databases

EnsemblBacteriaiAAG06128; AAG06128; PA2740.
GeneIDi882970.
KEGGipae:PA2740.
PATRICi19839981. VBIPseAer58763_2865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06128.1.
PIRiB83303.
RefSeqiNP_251430.1. NC_002516.2.
WP_003114409.1. NZ_ASJY01000445.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P71X-ray2.79C/D1-338[»]
4P72X-ray2.62C/D1-338[»]
4P73X-ray3.03C/D1-338[»]
4P74X-ray2.70C/D1-338[»]
4P75X-ray2.96C/D1-338[»]
ProteinModelPortaliQ9I0A3.
SMRiQ9I0A3. Positions 7-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA2740.

Proteomic databases

PaxDbiQ9I0A3.

Protocols and materials databases

DNASUi882970.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06128; AAG06128; PA2740.
GeneIDi882970.
KEGGipae:PA2740.
PATRICi19839981. VBIPseAer58763_2865.

Organism-specific databases

PseudoCAPiPA2740.

Phylogenomic databases

eggNOGiENOG4105CSS. Bacteria.
COG0016. LUCA.
HOGENOMiHOG000242675.
InParanoidiQ9I0A3.
KOiK01889.
OMAiEYHPARD.
PhylomeDBiQ9I0A3.

Family and domain databases

HAMAPiMF_00281. Phe_tRNA_synth_alpha1. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR004188. Phe-tRNA_ligase_II_N.
IPR022911. Phe_tRNA_ligase_alpha1_bac.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR010978. tRNA-bd_arm.
[Graphical view]
PfamiPF02912. Phe_tRNA-synt_N. 1 hit.
PF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYFA_PSEAE
AccessioniPrimary (citable) accession number: Q9I0A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.