Reviewed, UniProtKB/Swiss-Prot Q9I011 (TPMT_PSEAE)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	PA2832

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity HAMAP MF_00812.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_0000220126
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I011-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 3AFA7067401ED998	FASTA	218	24,875
	10 20 30 40 50 60 MQADFWHARW ANNQIGFHLD EINPYLMRHL SRLRLQAGEQ ILVPLCGKTL DLAWLAAQGL 70 80 90 100 110 120 EVLGVELSEK AVSDFFEEHD LHPEIDQLDG FRRYRVAGIT LLQGDFFALQ AEHLAQCRAF 130 140 150 160 170 180 YDRAALIALP PEMRERYAGH LQAVLPTRSL GLLVTIDYPQ AEMAGPPFAV PDEEVRGYYA 190 200 210 GGWRIEELER GDVLGVNWKF LERGVSWLDE AVYLLERG

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG06220.1.
PIR	A83291.
RefSeq	NP_251522.1.
3D structure databases
SMR	Q9I011. Positions 1-217.
ModBase	Search...
Genome annotation databases
GeneID	882816.
GenomeReviews	Gene locus PA2832 in contig AE004091_GR.
KEGG	pae:PA2832.
NMPDR	fig|208964.1.peg.2832.
Organism-specific databases
PseudoCAP	PA2832.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG444929.
OMA	GKSLDMC.
Enzyme and pathway databases
BioCyc	PAER208964:PA2832-MONOMER.
BRENDA	2.1.1.67. 354.
Family and domain databases
HAMAP	MF_00812. Thiopur_methtran. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_PSEAE

Accession

Primary (citable) accession number: Q9I011

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)