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Protein

Protein-glutamine gamma-glutamyltransferase

Gene

tgpA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Displays transglutaminase activity (TGase) in vitro. Plays a critical role in the viability of P.aeruginosa. Might contribute to an essential function linked to the cell wall.1 Publication

Catalytic activityi

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei404 – 4041NucleophileBy similarity
Active sitei448 – 4481By similarity
Active sitei464 – 4641By similarity

GO - Molecular functioni

  1. protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB

GO - Biological processi

  1. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-glutamine gamma-glutamyltransferase (EC:2.3.2.13)
Alternative name(s):
Transglutaminase protein A
Short name:
TGase A
Gene namesi
Name:tgpA
Ordered Locus Names:PA2873
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA2873.

Subcellular locationi

Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Transmembranei7 – 2721HelicalSequence AnalysisAdd
BLAST
Topological domaini28 – 5023PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei51 – 7121HelicalSequence AnalysisAdd
BLAST
Topological domaini72 – 743CytoplasmicSequence Analysis
Transmembranei75 – 9521HelicalSequence AnalysisAdd
BLAST
Topological domaini96 – 1038PeriplasmicSequence Analysis
Transmembranei104 – 12421HelicalSequence AnalysisAdd
BLAST
Transmembranei125 – 14521HelicalSequence AnalysisAdd
BLAST
Topological domaini146 – 15813CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei159 – 17921HelicalSequence AnalysisAdd
BLAST
Topological domaini180 – 548369PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei549 – 56921HelicalSequence AnalysisAdd
BLAST
Topological domaini570 – 66899CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption experiments show that tgpA is essential.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 668668Protein-glutamine gamma-glutamyltransferasePRO_0000422419Add
BLAST

Expressioni

Inductioni

Is expressed in both exponential and stationary phases of growth. Appears to be part of an operon together with PA2874 and PA2875.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi208964.PA2873.

Structurei

3D structure databases

ProteinModelPortaliQ9HZX3.
SMRiQ9HZX3. Positions 345-471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi59 – 187129Leu-richAdd
BLAST

Domaini

The transglutaminase activity is contained within the periplasmic domain (180-544).1 Publication

Sequence similaritiesi

Belongs to the transglutaminase-like superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000281216.
InParanoidiQ9HZX3.
OMAiQFDAHAW.
OrthoDBiEOG6Z0Q6F.
PhylomeDBiQ9HZX3.

Family and domain databases

Gene3Di3.90.260.10. 1 hit.
InterProiIPR021878. DUF3488.
IPR025403. DUF4129.
IPR002931. Transglutaminase-like.
[Graphical view]
PfamiPF11992. DUF3488. 1 hit.
PF13559. DUF4129. 1 hit.
PF01841. Transglut_core. 1 hit.
[Graphical view]
SMARTiSM00460. TGc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HZX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAIPRVALV WLLVAQVLVI LPHLAYMPLW IAAMWLGCAA WRVQVFRMRA
60 70 80 90 100
GYPRAWVKLA LALLAGAGVW LSRGSLVGLD AGAVLLIAAF ILKLVEMKTR
110 120 130 140 150
RDALVLVFLG FFAVVVGYLF DDGFLAALYS LLPVTALLAA LIGLQQSAFA
160 170 180 190 200
SRPWPTLRLA GGLLLQALPL MLLLFLFFPR LGPLWSLPMP GNKGVTGLSE
210 220 230 240 250
SMAPGDIAEL GRSAELAFRV RFEGALPPRE QLYWRALTME RFDGRRWAQA
260 270 280 290 300
PQWSGEDALH WQKRGPELRY DVIMQPSSQP WLFALDVAQT DQTDTRLMSD
310 320 330 340 350
FHLQRRQPVE QRLFYRVSSW PQALRESSID PRTRWRNLQL PMHGNPRARA
360 370 380 390 400
LADELRQAHA QPQALVAALL QRFNHEPFAY TLKPPATGAD GVDDFLFDTR
410 420 430 440 450
SGFCAHYAGA MAFVLRAAGI PARVVAGYQG GELNPAGNYL LVHQFDAHAW
460 470 480 490 500
VEYWQPEQGW LSVDPTYQVA PERIEQGLEQ ALAGDSEYLA DAPLSPLRYR
510 520 530 540 550
GLPWLNDMRL AWDSLNYGWQ RWVLAYQGEQ QGAFLQRWFG GLDPTRLGLL
560 570 580 590 600
LGAAAILSVG LLALFLLKPW QGRGDLRSRQ LRRFERLLEM HGLRRSPGEG
610 620 630 640 650
LRSYGERAAR VLPAQAPAIA AFVGAFEAQR YGHGGADDPG LRLRALRRAL
660
PWRLVRTPTR DGRGEEQA
Length:668
Mass (Da):74,807
Last modified:February 28, 2001 - v1
Checksum:i646CD6D34F6E96D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06261.1.
PIRiF83287.
RefSeqiNP_251563.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06261; AAG06261; PA2873.
GeneIDi882644.
KEGGipae:PA2873.
PATRICi19840289. VBIPseAer58763_3014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06261.1.
PIRiF83287.
RefSeqiNP_251563.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ9HZX3.
SMRiQ9HZX3. Positions 345-471.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA2873.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06261; AAG06261; PA2873.
GeneIDi882644.
KEGGipae:PA2873.
PATRICi19840289. VBIPseAer58763_3014.

Organism-specific databases

PseudoCAPiPA2873.

Phylogenomic databases

HOGENOMiHOG000281216.
InParanoidiQ9HZX3.
OMAiQFDAHAW.
OrthoDBiEOG6Z0Q6F.
PhylomeDBiQ9HZX3.

Family and domain databases

Gene3Di3.90.260.10. 1 hit.
InterProiIPR021878. DUF3488.
IPR025403. DUF4129.
IPR002931. Transglutaminase-like.
[Graphical view]
PfamiPF11992. DUF3488. 1 hit.
PF13559. DUF4129. 1 hit.
PF01841. Transglut_core. 1 hit.
[Graphical view]
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. "TgpA, a protein with a eukaryotic-like transglutaminase domain, plays a critical role in the viability of Pseudomonas aeruginosa."
    Milani A., Vecchietti D., Rusmini R., Bertoni G.
    PLoS ONE 7:E50323-E50323(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, GENE NAME, DOMAIN, SUBCELLULAR LOCATION, TOPOLOGY, DISRUPTION PHENOTYPE, INDUCTION.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiTGPA_PSEAE
AccessioniPrimary (citable) accession number: Q9HZX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 28, 2013
Last sequence update: February 28, 2001
Last modified: January 6, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Unlike Guinea pig TGase, TGase activity is not affected by EDTA addition, suggesting that TGase activity is Ca2+-independent, similar to members of the bacterial transglutaminase family.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.