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Q9HZX3

- TGPA_PSEAE

UniProt

Q9HZX3 - TGPA_PSEAE

Protein

Protein-glutamine gamma-glutamyltransferase

Gene

tgpA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Displays transglutaminase activity (TGase) in vitro. Plays a critical role in the viability of P.aeruginosa. Might contribute to an essential function linked to the cell wall.1 Publication

    Catalytic activityi

    Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei404 – 4041NucleophileBy similarity
    Active sitei448 – 4481By similarity
    Active sitei464 – 4641By similarity

    GO - Molecular functioni

    1. protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. metabolic process Source: GOC

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-glutamine gamma-glutamyltransferase (EC:2.3.2.13)
    Alternative name(s):
    Transglutaminase protein A
    Short name:
    TGase A
    Gene namesi
    Name:tgpA
    Ordered Locus Names:PA2873
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA2873.

    Subcellular locationi

    Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Disruption experiments show that tgpA is essential.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 668668Protein-glutamine gamma-glutamyltransferasePRO_0000422419Add
    BLAST

    Expressioni

    Inductioni

    Is expressed in both exponential and stationary phases of growth. Appears to be part of an operon together with PA2874 and PA2875.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA2873.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HZX3.
    SMRiQ9HZX3. Positions 345-471.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini28 – 5023PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini72 – 743CytoplasmicSequence Analysis
    Topological domaini96 – 1038PeriplasmicSequence Analysis
    Topological domaini146 – 15813CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini180 – 548369PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini570 – 66899CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei51 – 7121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei75 – 9521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei104 – 12421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei125 – 14521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei159 – 17921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei549 – 56921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi59 – 187129Leu-richAdd
    BLAST

    Domaini

    The transglutaminase activity is contained within the periplasmic domain (180-544).1 Publication

    Sequence similaritiesi

    Belongs to the transglutaminase-like superfamily.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000281216.
    OMAiQFDAHAW.
    OrthoDBiEOG6Z0Q6F.
    PhylomeDBiQ9HZX3.

    Family and domain databases

    Gene3Di3.90.260.10. 1 hit.
    InterProiIPR021878. DUF3488.
    IPR025403. DUF4129.
    IPR002931. Transglutaminase-like.
    [Graphical view]
    PfamiPF11992. DUF3488. 1 hit.
    PF13559. DUF4129. 1 hit.
    PF01841. Transglut_core. 1 hit.
    [Graphical view]
    SMARTiSM00460. TGc. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HZX3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNAIPRVALV WLLVAQVLVI LPHLAYMPLW IAAMWLGCAA WRVQVFRMRA    50
    GYPRAWVKLA LALLAGAGVW LSRGSLVGLD AGAVLLIAAF ILKLVEMKTR 100
    RDALVLVFLG FFAVVVGYLF DDGFLAALYS LLPVTALLAA LIGLQQSAFA 150
    SRPWPTLRLA GGLLLQALPL MLLLFLFFPR LGPLWSLPMP GNKGVTGLSE 200
    SMAPGDIAEL GRSAELAFRV RFEGALPPRE QLYWRALTME RFDGRRWAQA 250
    PQWSGEDALH WQKRGPELRY DVIMQPSSQP WLFALDVAQT DQTDTRLMSD 300
    FHLQRRQPVE QRLFYRVSSW PQALRESSID PRTRWRNLQL PMHGNPRARA 350
    LADELRQAHA QPQALVAALL QRFNHEPFAY TLKPPATGAD GVDDFLFDTR 400
    SGFCAHYAGA MAFVLRAAGI PARVVAGYQG GELNPAGNYL LVHQFDAHAW 450
    VEYWQPEQGW LSVDPTYQVA PERIEQGLEQ ALAGDSEYLA DAPLSPLRYR 500
    GLPWLNDMRL AWDSLNYGWQ RWVLAYQGEQ QGAFLQRWFG GLDPTRLGLL 550
    LGAAAILSVG LLALFLLKPW QGRGDLRSRQ LRRFERLLEM HGLRRSPGEG 600
    LRSYGERAAR VLPAQAPAIA AFVGAFEAQR YGHGGADDPG LRLRALRRAL 650
    PWRLVRTPTR DGRGEEQA 668
    Length:668
    Mass (Da):74,807
    Last modified:March 1, 2001 - v1
    Checksum:i646CD6D34F6E96D4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06261.1.
    PIRiF83287.
    RefSeqiNP_251563.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06261; AAG06261; PA2873.
    GeneIDi882644.
    KEGGipae:PA2873.
    PATRICi19840289. VBIPseAer58763_3014.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06261.1 .
    PIRi F83287.
    RefSeqi NP_251563.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali Q9HZX3.
    SMRi Q9HZX3. Positions 345-471.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA2873.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG06261 ; AAG06261 ; PA2873 .
    GeneIDi 882644.
    KEGGi pae:PA2873.
    PATRICi 19840289. VBIPseAer58763_3014.

    Organism-specific databases

    PseudoCAPi PA2873.

    Phylogenomic databases

    HOGENOMi HOG000281216.
    OMAi QFDAHAW.
    OrthoDBi EOG6Z0Q6F.
    PhylomeDBi Q9HZX3.

    Family and domain databases

    Gene3Di 3.90.260.10. 1 hit.
    InterProi IPR021878. DUF3488.
    IPR025403. DUF4129.
    IPR002931. Transglutaminase-like.
    [Graphical view ]
    Pfami PF11992. DUF3488. 1 hit.
    PF13559. DUF4129. 1 hit.
    PF01841. Transglut_core. 1 hit.
    [Graphical view ]
    SMARTi SM00460. TGc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. "TgpA, a protein with a eukaryotic-like transglutaminase domain, plays a critical role in the viability of Pseudomonas aeruginosa."
      Milani A., Vecchietti D., Rusmini R., Bertoni G.
      PLoS ONE 7:E50323-E50323(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, GENE NAME, DOMAIN, SUBCELLULAR LOCATION, TOPOLOGY, DISRUPTION PHENOTYPE, INDUCTION.
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiTGPA_PSEAE
    AccessioniPrimary (citable) accession number: Q9HZX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2013
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Unlike Guinea pig TGase, TGase activity is not affected by EDTA addition, suggesting that TGase activity is Ca2+-independent, similar to members of the bacterial transglutaminase family.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3