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Protein

Protein-glutamine gamma-glutamyltransferase

Gene

tgpA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Displays transglutaminase activity (TGase) in vitro. Plays a critical role in the viability of P.aeruginosa. Might contribute to an essential function linked to the cell wall.1 Publication

Miscellaneous

Unlike Guinea pig TGase, TGase activity is not affected by EDTA addition, suggesting that TGase activity is Ca2+-independent, similar to members of the bacterial transglutaminase family.1 Publication

Catalytic activityi

A protein-L-glutamine + a protein-L-lysine = a protein with an N6-(gamma-glutamyl)-L-lysine cross-link + NH3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei404NucleophileBy similarity1
Active sitei448By similarity1
Active sitei464By similarity1

GO - Molecular functioni

  • protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-2923-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-glutamine gamma-glutamyltransferase (EC:2.3.2.13)
Alternative name(s):
Transglutaminase protein A
Short name:
TGase A
Gene namesi
Name:tgpA
Ordered Locus Names:PA2873
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA2873

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Topological domaini28 – 50PeriplasmicSequence analysisAdd BLAST23
Transmembranei51 – 71HelicalSequence analysisAdd BLAST21
Topological domaini72 – 74CytoplasmicSequence analysis3
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Topological domaini96 – 103PeriplasmicSequence analysis8
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Transmembranei125 – 145HelicalSequence analysisAdd BLAST21
Topological domaini146 – 158CytoplasmicSequence analysisAdd BLAST13
Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
Topological domaini180 – 548PeriplasmicSequence analysisAdd BLAST369
Transmembranei549 – 569HelicalSequence analysisAdd BLAST21
Topological domaini570 – 668CytoplasmicSequence analysisAdd BLAST99

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption experiments show that tgpA is essential.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004224191 – 668Protein-glutamine gamma-glutamyltransferaseAdd BLAST668

Proteomic databases

PaxDbiQ9HZX3

Expressioni

Inductioni

Is expressed in both exponential and stationary phases of growth. Appears to be part of an operon together with PA2874 and PA2875.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi208964.PA2873

Structurei

3D structure databases

ProteinModelPortaliQ9HZX3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi59 – 187Leu-richAdd BLAST129

Domaini

The transglutaminase activity is contained within the periplasmic domain (180-544).1 Publication

Sequence similaritiesi

Belongs to the transglutaminase-like superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105F8C Bacteria
COG1305 LUCA
HOGENOMiHOG000281216
InParanoidiQ9HZX3
KOiK22452
OMAiQFDAHAW
PhylomeDBiQ9HZX3

Family and domain databases

InterProiView protein in InterPro
IPR021878 DUF3488
IPR025403 DUF4129
IPR002931 Transglutaminase-like
PfamiView protein in Pfam
PF11992 DUF3488, 1 hit
PF13559 DUF4129, 1 hit
PF01841 Transglut_core, 1 hit
SMARTiView protein in SMART
SM00460 TGc, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HZX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAIPRVALV WLLVAQVLVI LPHLAYMPLW IAAMWLGCAA WRVQVFRMRA
60 70 80 90 100
GYPRAWVKLA LALLAGAGVW LSRGSLVGLD AGAVLLIAAF ILKLVEMKTR
110 120 130 140 150
RDALVLVFLG FFAVVVGYLF DDGFLAALYS LLPVTALLAA LIGLQQSAFA
160 170 180 190 200
SRPWPTLRLA GGLLLQALPL MLLLFLFFPR LGPLWSLPMP GNKGVTGLSE
210 220 230 240 250
SMAPGDIAEL GRSAELAFRV RFEGALPPRE QLYWRALTME RFDGRRWAQA
260 270 280 290 300
PQWSGEDALH WQKRGPELRY DVIMQPSSQP WLFALDVAQT DQTDTRLMSD
310 320 330 340 350
FHLQRRQPVE QRLFYRVSSW PQALRESSID PRTRWRNLQL PMHGNPRARA
360 370 380 390 400
LADELRQAHA QPQALVAALL QRFNHEPFAY TLKPPATGAD GVDDFLFDTR
410 420 430 440 450
SGFCAHYAGA MAFVLRAAGI PARVVAGYQG GELNPAGNYL LVHQFDAHAW
460 470 480 490 500
VEYWQPEQGW LSVDPTYQVA PERIEQGLEQ ALAGDSEYLA DAPLSPLRYR
510 520 530 540 550
GLPWLNDMRL AWDSLNYGWQ RWVLAYQGEQ QGAFLQRWFG GLDPTRLGLL
560 570 580 590 600
LGAAAILSVG LLALFLLKPW QGRGDLRSRQ LRRFERLLEM HGLRRSPGEG
610 620 630 640 650
LRSYGERAAR VLPAQAPAIA AFVGAFEAQR YGHGGADDPG LRLRALRRAL
660
PWRLVRTPTR DGRGEEQA
Length:668
Mass (Da):74,807
Last modified:March 1, 2001 - v1
Checksum:i646CD6D34F6E96D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG06261.1
PIRiF83287
RefSeqiNP_251563.1, NC_002516.2
WP_003114743.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG06261; AAG06261; PA2873
GeneIDi882644
KEGGipae:PA2873
PATRICifig|208964.12.peg.3014

Similar proteinsi

Entry informationi

Entry nameiTGPA_PSEAE
AccessioniPrimary (citable) accession number: Q9HZX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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