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Protein

UDP-N-acetylenolpyruvoylglucosamine reductase

Gene

murB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

UDP-N-acetyl-alpha-D-muramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH.UniRule annotation

Cofactori

FADUniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei166UniRule annotation1
Active sitei239Proton donorUniRule annotation1
Active sitei335UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductaseUniRule annotation (EC:1.3.1.98UniRule annotation)
Alternative name(s):
UDP-N-acetylmuramate dehydrogenaseUniRule annotation
Gene namesi
Name:murBUniRule annotation
Ordered Locus Names:PA2977
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA2977.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001792421 – 339UDP-N-acetylenolpyruvoylglucosamine reductaseAdd BLAST339

Proteomic databases

PaxDbiQ9HZM7.
PRIDEiQ9HZM7.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA2977.

Structurei

Secondary structure

1339
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Helixi12 – 14Combined sources3
Beta strandi22 – 29Combined sources8
Helixi32 – 44Combined sources13
Beta strandi49 – 55Combined sources7
Beta strandi56 – 60Combined sources5
Beta strandi64 – 71Combined sources8
Beta strandi76 – 81Combined sources6
Beta strandi83 – 91Combined sources9
Helixi96 – 105Combined sources10
Helixi112 – 114Combined sources3
Turni121 – 128Combined sources8
Helixi136 – 139Combined sources4
Beta strandi140 – 147Combined sources8
Turni148 – 150Combined sources3
Beta strandi153 – 157Combined sources5
Helixi159 – 161Combined sources3
Helixi169 – 172Combined sources4
Turni174 – 176Combined sources3
Beta strandi177 – 189Combined sources13
Helixi197 – 205Combined sources9
Helixi213 – 227Combined sources15
Turni231 – 233Combined sources3
Helixi248 – 257Combined sources10
Beta strandi263 – 265Combined sources3
Beta strandi271 – 273Combined sources3
Helixi275 – 281Combined sources7
Beta strandi292 – 294Combined sources3
Beta strandi301 – 304Combined sources4
Helixi310 – 328Combined sources19
Beta strandi336 – 338Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JAYX-ray2.23A/B/C/D1-339[»]
4JB1X-ray2.10A1-339[»]
ProteinModelPortaliQ9HZM7.
SMRiQ9HZM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 189FAD-binding PCMH-typeUniRule annotationAdd BLAST172

Sequence similaritiesi

Belongs to the MurB family.UniRule annotation
Contains 1 FAD-binding PCMH-type domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D4A. Bacteria.
COG0812. LUCA.
HOGENOMiHOG000284356.
InParanoidiQ9HZM7.
KOiK00075.
OMAiMQNIGAY.
PhylomeDBiQ9HZM7.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.78.10. 1 hit.
HAMAPiMF_00037. MurB. 1 hit.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR003170. MurB.
IPR011601. MurB_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR21071. PTHR21071. 1 hit.
PfamiPF01565. FAD_binding_4. 1 hit.
PF02873. MurB_C. 1 hit.
[Graphical view]
SUPFAMiSSF56176. SSF56176. 1 hit.
SSF56194. SSF56194. 1 hit.
TIGRFAMsiTIGR00179. murB. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HZM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLELQEHCS LKPYNTFGID VRARLLAHAR DEADVREALA LARERGLPLL
60 70 80 90 100
VIGGGSNLLL TRDVEALVLR MASQGRRIVS DAADSVLVEA EAGEAWDPFV
110 120 130 140 150
QWSLERGLAG LENLSLIPGT VGAAPMQNIG AYGVELKDVF DSLTALDRQD
160 170 180 190 200
GTLREFDRQA CRFGYRDSLF KQEPDRWLIL RVRLRLTRRE RLHLDYGPVR
210 220 230 240 250
QRLEEEGIAS PTARDVSRVI CAIRREKLPD PAVLGNAGSF FKNPLVDATQ
260 270 280 290 300
AERLRQAFPD LVGYPQADGR LKLAAGWLID KGGWKGFRDG PVGVHAQQAL
310 320 330
VLVNHGGATG AQVRALAERI QEDVRRRFGV ELEPEPNLY
Length:339
Mass (Da):37,628
Last modified:March 1, 2001 - v1
Checksum:iC0C8EF9F2938FE27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06365.1.
PIRiA83274.
RefSeqiNP_251667.1. NC_002516.2.
WP_003106928.1. NZ_ASJY01000490.1.

Genome annotation databases

EnsemblBacteriaiAAG06365; AAG06365; PA2977.
GeneIDi880517.
KEGGipae:PA2977.
PATRICi19840509. VBIPseAer58763_3124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06365.1.
PIRiA83274.
RefSeqiNP_251667.1. NC_002516.2.
WP_003106928.1. NZ_ASJY01000490.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JAYX-ray2.23A/B/C/D1-339[»]
4JB1X-ray2.10A1-339[»]
ProteinModelPortaliQ9HZM7.
SMRiQ9HZM7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA2977.

Proteomic databases

PaxDbiQ9HZM7.
PRIDEiQ9HZM7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06365; AAG06365; PA2977.
GeneIDi880517.
KEGGipae:PA2977.
PATRICi19840509. VBIPseAer58763_3124.

Organism-specific databases

PseudoCAPiPA2977.

Phylogenomic databases

eggNOGiENOG4105D4A. Bacteria.
COG0812. LUCA.
HOGENOMiHOG000284356.
InParanoidiQ9HZM7.
KOiK00075.
OMAiMQNIGAY.
PhylomeDBiQ9HZM7.

Enzyme and pathway databases

UniPathwayiUPA00219.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.78.10. 1 hit.
HAMAPiMF_00037. MurB. 1 hit.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR003170. MurB.
IPR011601. MurB_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR21071. PTHR21071. 1 hit.
PfamiPF01565. FAD_binding_4. 1 hit.
PF02873. MurB_C. 1 hit.
[Graphical view]
SUPFAMiSSF56176. SSF56176. 1 hit.
SSF56194. SSF56194. 1 hit.
TIGRFAMsiTIGR00179. murB. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMURB_PSEAE
AccessioniPrimary (citable) accession number: Q9HZM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.