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Protein

S-methyl-5'-thioinosine phosphorylase

Gene

PA3004

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine. Involved in quorum sensing.UniRule annotation1 Publication

Catalytic activityi

S-methyl-5'-thioinosine + phosphate = hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Kineticsi

  1. KM=2.6 µM for S-methyl-5'-thioinosine1 Publication
  2. KM=23 µM for inosine1 Publication
  3. KM=90 µM for adenosine1 Publication

    Pathwayi: purine nucleoside salvage

    This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.UniRule annotation
    View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei10PhosphateUniRule annotation1
    Sitei167Important for substrate specificity1
    Binding sitei185Substrate; via amide nitrogenUniRule annotation1
    Binding sitei186PhosphateUniRule annotation1
    Sitei221Important for substrate specificityUniRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16355.
    BRENDAi2.4.2.44. 5087.
    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-methyl-5'-thioinosine phosphorylase (EC:2.4.2.44UniRule annotation)
    Alternative name(s):
    5'-methylthioinosine phosphorylaseUniRule annotation
    Short name:
    MTI phosphorylaseUniRule annotation
    Short name:
    MTIPUniRule annotation
    Gene namesi
    Ordered Locus Names:PA3004
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    Proteomesi
    • UP000002438 Componenti: Chromosome

    Organism-specific databases

    PseudoCAPiPA3004.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001845511 – 245S-methyl-5'-thioinosine phosphorylaseAdd BLAST245

    Proteomic databases

    PaxDbiQ9HZK1.
    PRIDEiQ9HZK1.

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRINGi208964.PA3004.

    Structurei

    Secondary structure

    1245
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 8Combined sources5
    Beta strandi37 – 42Combined sources6
    Beta strandi45 – 53Combined sources9
    Helixi61 – 63Combined sources3
    Helixi66 – 76Combined sources11
    Beta strandi79 – 89Combined sources11
    Beta strandi103 – 107Combined sources5
    Beta strandi116 – 120Combined sources5
    Helixi135 – 147Combined sources13
    Beta strandi152 – 154Combined sources3
    Beta strandi157 – 160Combined sources4
    Helixi169 – 177Combined sources9
    Beta strandi181 – 187Combined sources7
    Helixi190 – 196Combined sources7
    Beta strandi201 – 210Combined sources10
    Turni212 – 214Combined sources3
    Beta strandi215 – 217Combined sources3
    Helixi221 – 243Combined sources23

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3OZBX-ray2.80A/B/C/D/E/F1-245[»]
    ProteinModelPortaliQ9HZK1.
    SMRiQ9HZK1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni52 – 53Phosphate bindingUniRule annotation2
    Regioni209 – 211Substrate binding3

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4107RHB. Bacteria.
    COG0005. LUCA.
    HOGENOMiHOG000228987.
    InParanoidiQ9HZK1.
    KOiK19696.
    OMAiGCDIVGM.
    PhylomeDBiQ9HZK1.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP. 1 hit.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HZK1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSVYAIIGGT GLTQLEGLTL SESLPIETPY GAPSAPLQRG RYAGREVLFL
    60 70 80 90 100
    ARHGHPHRFP PHQVNYRANL WALKQAGAEA VIAVNAVGGI HAAMGTGHLC
    110 120 130 140 150
    VPHQLIDYTS GREHTYFAGD IEHVTHIDFS HPYDEPLRQR LIEALRALGL
    160 170 180 190 200
    AHSSHGVYAC TQGPRLETVA EIARLERDGN DIVGMTGMPE AALARELDLP
    210 220 230 240
    YACLALVVNP AAGKSAGIIT MAEIEQALHD GIGKVREVLA RVLAG
    Length:245
    Mass (Da):26,266
    Last modified:March 1, 2001 - v1
    Checksum:i3C8442062D0666E6
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06392.1.
    PIRiF83270.
    RefSeqiNP_251694.1. NC_002516.2.
    WP_003091193.1. NZ_ASJY01000491.1.

    Genome annotation databases

    EnsemblBacteriaiAAG06392; AAG06392; PA3004.
    GeneIDi880215.
    KEGGipae:PA3004.
    PATRICi19840565. VBIPseAer58763_3152.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06392.1.
    PIRiF83270.
    RefSeqiNP_251694.1. NC_002516.2.
    WP_003091193.1. NZ_ASJY01000491.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3OZBX-ray2.80A/B/C/D/E/F1-245[»]
    ProteinModelPortaliQ9HZK1.
    SMRiQ9HZK1.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi208964.PA3004.

    Proteomic databases

    PaxDbiQ9HZK1.
    PRIDEiQ9HZK1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAG06392; AAG06392; PA3004.
    GeneIDi880215.
    KEGGipae:PA3004.
    PATRICi19840565. VBIPseAer58763_3152.

    Organism-specific databases

    PseudoCAPiPA3004.

    Phylogenomic databases

    eggNOGiENOG4107RHB. Bacteria.
    COG0005. LUCA.
    HOGENOMiHOG000228987.
    InParanoidiQ9HZK1.
    KOiK19696.
    OMAiGCDIVGM.
    PhylomeDBiQ9HZK1.

    Enzyme and pathway databases

    UniPathwayiUPA00606.
    BioCyciMetaCyc:MONOMER-16355.
    BRENDAi2.4.2.44. 5087.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP. 1 hit.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMTIP_PSEAE
    AccessioniPrimary (citable) accession number: Q9HZK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: March 1, 2001
    Last modified: November 2, 2016
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.