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Protein

S-methyl-5'-thioinosine phosphorylase

Gene

PA3004

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine. Involved in quorum sensing.UniRule annotation1 Publication

Catalytic activityi

S-methyl-5'-thioinosine + phosphate = hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Kineticsi

  1. KM=2.6 µM for S-methyl-5'-thioinosine1 Publication
  2. KM=23 µM for inosine1 Publication
  3. KM=90 µM for adenosine1 Publication

    Pathwayi: purine nucleoside salvage

    This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.UniRule annotation
    View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101PhosphateUniRule annotation
    Sitei167 – 1671Important for substrate specificity
    Binding sitei185 – 1851Substrate; via amide nitrogenUniRule annotation
    Binding sitei186 – 1861PhosphateUniRule annotation
    Sitei221 – 2211Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16355.
    BRENDAi2.4.2.44. 5087.
    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-methyl-5'-thioinosine phosphorylase (EC:2.4.2.44UniRule annotation)
    Alternative name(s):
    5'-methylthioinosine phosphorylaseUniRule annotation
    Short name:
    MTI phosphorylaseUniRule annotation
    Short name:
    MTIPUniRule annotation
    Gene namesi
    Ordered Locus Names:PA3004
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    Proteomesi
    • UP000002438 Componenti: Chromosome

    Organism-specific databases

    PseudoCAPiPA3004.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 245245S-methyl-5'-thioinosine phosphorylasePRO_0000184551Add
    BLAST

    Proteomic databases

    PaxDbiQ9HZK1.

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRINGi208964.PA3004.

    Structurei

    Secondary structure

    1
    245
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 85Combined sources
    Beta strandi37 – 426Combined sources
    Beta strandi45 – 539Combined sources
    Helixi61 – 633Combined sources
    Helixi66 – 7611Combined sources
    Beta strandi79 – 8911Combined sources
    Beta strandi103 – 1075Combined sources
    Beta strandi116 – 1205Combined sources
    Helixi135 – 14713Combined sources
    Beta strandi152 – 1543Combined sources
    Beta strandi157 – 1604Combined sources
    Helixi169 – 1779Combined sources
    Beta strandi181 – 1877Combined sources
    Helixi190 – 1967Combined sources
    Beta strandi201 – 21010Combined sources
    Turni212 – 2143Combined sources
    Beta strandi215 – 2173Combined sources
    Helixi221 – 24323Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3OZBX-ray2.80A/B/C/D/E/F1-245[»]
    ProteinModelPortaliQ9HZK1.
    SMRiQ9HZK1. Positions 1-244.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni52 – 532Phosphate bindingUniRule annotation
    Regioni209 – 2113Substrate binding

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4107RHB. Bacteria.
    COG0005. LUCA.
    HOGENOMiHOG000228987.
    InParanoidiQ9HZK1.
    KOiK19696.
    OMAiGCDIVGM.
    PhylomeDBiQ9HZK1.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP. 1 hit.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HZK1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSVYAIIGGT GLTQLEGLTL SESLPIETPY GAPSAPLQRG RYAGREVLFL
    60 70 80 90 100
    ARHGHPHRFP PHQVNYRANL WALKQAGAEA VIAVNAVGGI HAAMGTGHLC
    110 120 130 140 150
    VPHQLIDYTS GREHTYFAGD IEHVTHIDFS HPYDEPLRQR LIEALRALGL
    160 170 180 190 200
    AHSSHGVYAC TQGPRLETVA EIARLERDGN DIVGMTGMPE AALARELDLP
    210 220 230 240
    YACLALVVNP AAGKSAGIIT MAEIEQALHD GIGKVREVLA RVLAG
    Length:245
    Mass (Da):26,266
    Last modified:March 1, 2001 - v1
    Checksum:i3C8442062D0666E6
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06392.1.
    PIRiF83270.
    RefSeqiNP_251694.1. NC_002516.2.
    WP_003091193.1. NZ_ASJY01000491.1.

    Genome annotation databases

    EnsemblBacteriaiAAG06392; AAG06392; PA3004.
    GeneIDi880215.
    KEGGipae:PA3004.
    PATRICi19840565. VBIPseAer58763_3152.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06392.1.
    PIRiF83270.
    RefSeqiNP_251694.1. NC_002516.2.
    WP_003091193.1. NZ_ASJY01000491.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3OZBX-ray2.80A/B/C/D/E/F1-245[»]
    ProteinModelPortaliQ9HZK1.
    SMRiQ9HZK1. Positions 1-244.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi208964.PA3004.

    Proteomic databases

    PaxDbiQ9HZK1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAG06392; AAG06392; PA3004.
    GeneIDi880215.
    KEGGipae:PA3004.
    PATRICi19840565. VBIPseAer58763_3152.

    Organism-specific databases

    PseudoCAPiPA3004.

    Phylogenomic databases

    eggNOGiENOG4107RHB. Bacteria.
    COG0005. LUCA.
    HOGENOMiHOG000228987.
    InParanoidiQ9HZK1.
    KOiK19696.
    OMAiGCDIVGM.
    PhylomeDBiQ9HZK1.

    Enzyme and pathway databases

    UniPathwayiUPA00606.
    BioCyciMetaCyc:MONOMER-16355.
    BRENDAi2.4.2.44. 5087.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP. 1 hit.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMTIP_PSEAE
    AccessioniPrimary (citable) accession number: Q9HZK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: March 1, 2001
    Last modified: September 7, 2016
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.