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Q9HZJ5

- TOP1_PSEAE

UniProt

Q9HZJ5 - TOP1_PSEAE

Protein

DNA topoisomerase 1

Gene

topA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
    Sitei33 – 331Interaction with DNAUniRule annotation
    Metal bindingi116 – 1161Magnesium 1; catalyticUniRule annotation
    Metal bindingi116 – 1161Magnesium 2UniRule annotation
    Metal bindingi118 – 1181Magnesium 2UniRule annotation
    Sitei173 – 1731Interaction with DNAUniRule annotation
    Sitei174 – 1741Interaction with DNAUniRule annotation
    Sitei177 – 1771Interaction with DNAUniRule annotation
    Sitei182 – 1821Interaction with DNAUniRule annotation
    Sitei189 – 1891Interaction with DNAUniRule annotation
    Active sitei324 – 3241O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei326 – 3261Interaction with DNAUniRule annotation
    Sitei512 – 5121Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri602 – 63332C4-type 1Add
    BLAST
    Zinc fingeri664 – 69128C4-type 2Add
    BLAST
    Zinc fingeri713 – 73826C4-type 3Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
    4. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:PA3011
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA3011.

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 868868DNA topoisomerase 1PRO_0000145161Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi208964.PA3011.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HZJ5.
    SMRiQ9HZJ5. Positions 2-652, 747-868.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 147145ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni197 – 2026Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri602 – 63332C4-type 1Add
    BLAST
    Zinc fingeri664 – 69128C4-type 2Add
    BLAST
    Zinc fingeri713 – 73826C4-type 3Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0551.
    HOGENOMiHOG000004018.
    KOiK03168.
    OMAiVVECDKC.
    OrthoDBiEOG6S7XQ9.
    PhylomeDBiQ9HZJ5.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR013263. TopoI_Znr_bac.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
    PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HZJ5-1 [UniParc]FASTAAdd to Basket

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    MGKSLVIVES PAKAKTINKY LGSQYVVKSS IGHIRDLPTS GSSSSKEPAA    50
    KGRKSASEAP ALSPKEKARR QLVSRMGVDP EHGWKAKYEI LPGKEKVIDE 100
    LRRLAKDADT VYLATDLDRE GEAIAWHLRE AIGGDESRYK RVVFNEITKK 150
    AIQEAFSQPG ELDINRVNAQ QARRFLDRVV GYMVSPLLWQ KIARGLSAGR 200
    VQSVAVKLVV EREREIRAFV PEEYWEVHAD LGTAKGANVR FEVTREKGEA 250
    FKPLNEAQAM AALEKLKASA YSVAKREDRP TSSRPSAPFI TSTLQQAASN 300
    RLGFGVKKTM MMAQRLYEAG YITYMRTDST NLSADAIGMV RGFIEDEFGQ 350
    KYLPGKANVY SSKEGAQEAH EAIRPSDVNL KPTQLSGMER DAERLYDLIW 400
    RQFVACQMTP AEYLSTSVSV TAGDFELRAK GRILKFDGYT RVLPQQSKPG 450
    EDDVLPEMKE GENLKLIKLD PSQHFTKPPA RYSEASLVKE LEKRGIGRPS 500
    TYAAIISTIQ ERGYVTTHNR RFYAEKMGDI VTDRLNESFA NLMDYGFTAG 550
    MEEHLDDVAQ GERDWKHLLD EFYGDFKKKL EVAEVSEKGM RANQPTLTNI 600
    PCRECGRPMM IRTASTGVFL GCSGYSLPPK ERCKATVNLI PGDEIAADDE 650
    GESESRVLRG KHRCPICSTA MDAYLLDEKH KLHICGNNPD CPGYEIEEGQ 700
    YRIKGYEGPS LECDKCGSEM QLKTGRFGKF FGCTNPTCKN TRKLLKNGEA 750
    APPKMDAIRM PELKCEKVDD IYVLRDGASG MFLAASQFPK NRETRAPLVS 800
    EIIPHKAELD PKYHYLCDAP QKDPDGRPAV IRFSRKTKEQ YVQSEVDGKP 850
    TGWRAFYDGG KWKVEDKR 868
    Length:868
    Mass (Da):97,283
    Last modified:March 1, 2001 - v1
    Checksum:i5EB4B8483366C89B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06399.1.
    PIRiD83269.
    RefSeqiNP_251701.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06399; AAG06399; PA3011.
    GeneIDi880353.
    KEGGipae:PA3011.
    PATRICi19840579. VBIPseAer58763_3159.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06399.1 .
    PIRi D83269.
    RefSeqi NP_251701.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali Q9HZJ5.
    SMRi Q9HZJ5. Positions 2-652, 747-868.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA3011.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG06399 ; AAG06399 ; PA3011 .
    GeneIDi 880353.
    KEGGi pae:PA3011.
    PATRICi 19840579. VBIPseAer58763_3159.

    Organism-specific databases

    PseudoCAPi PA3011.

    Phylogenomic databases

    eggNOGi COG0551.
    HOGENOMi HOG000004018.
    KOi K03168.
    OMAi VVECDKC.
    OrthoDBi EOG6S7XQ9.
    PhylomeDBi Q9HZJ5.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR013263. TopoI_Znr_bac.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
    PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiTOP1_PSEAE
    AccessioniPrimary (citable) accession number: Q9HZJ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3