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Q9HZJ5 (TOP1_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:PA3011
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length868 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 868868DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145161

Regions

Domain3 – 147145Toprim
Zinc finger602 – 63332C4-type 1 HAMAP-Rule MF_00952
Zinc finger664 – 69128C4-type 2 HAMAP-Rule MF_00952
Zinc finger713 – 73826C4-type 3 HAMAP-Rule MF_00952
Region197 – 2026Interaction with DNA By similarity

Sites

Active site3241O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium 1; catalytic By similarity
Metal binding1161Magnesium 1; catalytic By similarity
Metal binding1161Magnesium 2 By similarity
Metal binding1181Magnesium 2 By similarity
Site331Interaction with DNA By similarity
Site1731Interaction with DNA By similarity
Site1741Interaction with DNA By similarity
Site1771Interaction with DNA By similarity
Site1821Interaction with DNA By similarity
Site1891Interaction with DNA By similarity
Site3261Interaction with DNA By similarity
Site5121Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9HZJ5 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 5EB4B8483366C89B

FASTA86897,283
        10         20         30         40         50         60 
MGKSLVIVES PAKAKTINKY LGSQYVVKSS IGHIRDLPTS GSSSSKEPAA KGRKSASEAP 

        70         80         90        100        110        120 
ALSPKEKARR QLVSRMGVDP EHGWKAKYEI LPGKEKVIDE LRRLAKDADT VYLATDLDRE 

       130        140        150        160        170        180 
GEAIAWHLRE AIGGDESRYK RVVFNEITKK AIQEAFSQPG ELDINRVNAQ QARRFLDRVV 

       190        200        210        220        230        240 
GYMVSPLLWQ KIARGLSAGR VQSVAVKLVV EREREIRAFV PEEYWEVHAD LGTAKGANVR 

       250        260        270        280        290        300 
FEVTREKGEA FKPLNEAQAM AALEKLKASA YSVAKREDRP TSSRPSAPFI TSTLQQAASN 

       310        320        330        340        350        360 
RLGFGVKKTM MMAQRLYEAG YITYMRTDST NLSADAIGMV RGFIEDEFGQ KYLPGKANVY 

       370        380        390        400        410        420 
SSKEGAQEAH EAIRPSDVNL KPTQLSGMER DAERLYDLIW RQFVACQMTP AEYLSTSVSV 

       430        440        450        460        470        480 
TAGDFELRAK GRILKFDGYT RVLPQQSKPG EDDVLPEMKE GENLKLIKLD PSQHFTKPPA 

       490        500        510        520        530        540 
RYSEASLVKE LEKRGIGRPS TYAAIISTIQ ERGYVTTHNR RFYAEKMGDI VTDRLNESFA 

       550        560        570        580        590        600 
NLMDYGFTAG MEEHLDDVAQ GERDWKHLLD EFYGDFKKKL EVAEVSEKGM RANQPTLTNI 

       610        620        630        640        650        660 
PCRECGRPMM IRTASTGVFL GCSGYSLPPK ERCKATVNLI PGDEIAADDE GESESRVLRG 

       670        680        690        700        710        720 
KHRCPICSTA MDAYLLDEKH KLHICGNNPD CPGYEIEEGQ YRIKGYEGPS LECDKCGSEM 

       730        740        750        760        770        780 
QLKTGRFGKF FGCTNPTCKN TRKLLKNGEA APPKMDAIRM PELKCEKVDD IYVLRDGASG 

       790        800        810        820        830        840 
MFLAASQFPK NRETRAPLVS EIIPHKAELD PKYHYLCDAP QKDPDGRPAV IRFSRKTKEQ 

       850        860 
YVQSEVDGKP TGWRAFYDGG KWKVEDKR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004091 Genomic DNA. Translation: AAG06399.1.
PIRD83269.
RefSeqNP_251701.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9HZJ5.
SMRQ9HZJ5. Positions 2-652, 708-868.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA3011.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID880353.
KEGGpae:PA3011.
PATRIC19840579. VBIPseAer58763_3159.

Organism-specific databases

PseudoCAPPA3011.

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004018.
KOK03168.
OMAMVYKMGR.
OrthoDBEOG6S7XQ9.
ProtClustDBPRK07561.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_PSEAE
AccessionPrimary (citable) accession number: Q9HZJ5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families