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Q9HYN5

- Q9HYN5_PSEAE

UniProt

Q9HYN5 - Q9HYN5_PSEAE

Protein
Submitted name:

Fucose-binding lectin PA-IIL

Gene

lecB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi22 – 221Calcium 1; via carbonyl oxygenImported
    Binding sitei24 – 241N-acetyl-D-glucosamineImported
    Metal bindingi96 – 961Calcium 2Imported
    Binding sitei97 – 971N-acetyl-D-glucosamineImported
    Metal bindingi100 – 1001Calcium 2Imported
    Binding sitei100 – 1001GalactoseImported
    Metal bindingi102 – 1021Calcium 1Imported
    Metal bindingi102 – 1021Calcium 2Imported
    Metal bindingi104 – 1041Calcium 1Imported
    Metal bindingi105 – 1051Calcium 1Imported
    Metal bindingi105 – 1051Calcium 2Imported
    Metal bindingi115 – 1151Calcium 1; via carbonyl oxygenImported
    Metal bindingi115 – 1151Calcium 2; via carbonyl oxygenImported
    Binding sitei115 – 1151Fucose; via amide nitrogen and carbonyl oxygenImported
    Binding sitei115 – 1151Mannose; via amide nitrogen and carbonyl oxygenImported

    GO - Molecular functioni

    1. carbohydrate binding Source: PseudoCAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. biofilm formation Source: PseudoCAP

    Keywords - Ligandi

    CalciumImported, LectinImported, Metal-bindingImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Fucose-binding lectin PA-IILImported
    Gene namesi
    Name:lecBImported
    Ordered Locus Names:PA3361Imported
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)Imported
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA3361.

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA3361.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GZTX-ray1.30A/B/C/D1-115[»]
    1OURX-ray1.42A2-115[»]
    1OUSX-ray1.20A/B/C/D2-115[»]
    1OUXX-ray2.00A/B/C/D2-115[»]
    1OVPX-ray1.40A2-115[»]
    1OVSX-ray1.75A/B/C/D2-115[»]
    1OXCX-ray1.20A/B/C/D2-115[»]
    1UZVX-ray1.00A/B/C/D2-115[»]
    1W8FX-ray1.05A/B/C/D1-115[»]
    1W8HX-ray1.75A/B/C/D1-115[»]
    2BOJX-ray1.80A/B/C/D2-115[»]
    2BP6X-ray1.50A/B/C/D2-115[»]
    2JDHX-ray1.10A/B/C/D1-115[»]
    2JDKX-ray1.10A/B/C/D1-115[»]
    2JDMX-ray1.70A/B/C/D1-115[»]
    2JDNX-ray1.30A/B/C/D1-115[»]
    2JDPX-ray1.30A/B/C/D1-115[»]
    2JDUX-ray1.50A/B/C/D1-115[»]
    2JDYX-ray1.70A/B/C/D1-115[»]
    2VUCX-ray1.30A/B/C/D2-115[»]
    2VUDX-ray1.70A/B/C/D2-115[»]
    3DCQX-ray1.80A/B/C/D2-115[»]
    3ZDVX-ray1.41A/B/C/D1-115[»]
    ProteinModelPortaliQ9HYN5.
    SMRiQ9HYN5. Positions 2-115.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9HYN5.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 243Fucose bindingImported
    Regioni22 – 243Mannose bindingImported
    Regioni97 – 1059Fucose bindingImported
    Regioni97 – 1059Mannose bindingImported

    Phylogenomic databases

    HOGENOMiHOG000110720.
    OMAiNDYNDGI.
    OrthoDBiEOG67X1RS.

    Family and domain databases

    Gene3Di2.60.120.400. 1 hit.
    InterProiIPR010907. Ca-mediated_lectin.
    IPR016927. Lectin_sugar-bd.
    [Graphical view]
    PfamiPF07472. PA-IIL. 1 hit.
    [Graphical view]
    PIRSFiPIRSF029595. Lectin_LecB. 1 hit.
    SUPFAMiSSF82026. SSF82026. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9HYN5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATQGVFTLP ANTRFGVTAF ANSSGTQTVN VLVNNETAAT FSGQSTNNAV    50
    IGTQVLNSGS SGKVQVQVSV NGRPSDLVSA QVILTNELNF ALVGSEDGTD 100
    NDYNDAVVVI NWPLG 115
    Length:115
    Mass (Da):11,863
    Last modified:March 1, 2001 - v1
    Checksum:i616D8249512CDD7D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06749.1.
    PIRiH83225.
    RefSeqiNP_252051.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06749; AAG06749; PA3361.
    GeneIDi882528.
    KEGGipae:PA3361.
    PATRICi19841323. VBIPseAer58763_3520.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06749.1 .
    PIRi H83225.
    RefSeqi NP_252051.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GZT X-ray 1.30 A/B/C/D 1-115 [» ]
    1OUR X-ray 1.42 A 2-115 [» ]
    1OUS X-ray 1.20 A/B/C/D 2-115 [» ]
    1OUX X-ray 2.00 A/B/C/D 2-115 [» ]
    1OVP X-ray 1.40 A 2-115 [» ]
    1OVS X-ray 1.75 A/B/C/D 2-115 [» ]
    1OXC X-ray 1.20 A/B/C/D 2-115 [» ]
    1UZV X-ray 1.00 A/B/C/D 2-115 [» ]
    1W8F X-ray 1.05 A/B/C/D 1-115 [» ]
    1W8H X-ray 1.75 A/B/C/D 1-115 [» ]
    2BOJ X-ray 1.80 A/B/C/D 2-115 [» ]
    2BP6 X-ray 1.50 A/B/C/D 2-115 [» ]
    2JDH X-ray 1.10 A/B/C/D 1-115 [» ]
    2JDK X-ray 1.10 A/B/C/D 1-115 [» ]
    2JDM X-ray 1.70 A/B/C/D 1-115 [» ]
    2JDN X-ray 1.30 A/B/C/D 1-115 [» ]
    2JDP X-ray 1.30 A/B/C/D 1-115 [» ]
    2JDU X-ray 1.50 A/B/C/D 1-115 [» ]
    2JDY X-ray 1.70 A/B/C/D 1-115 [» ]
    2VUC X-ray 1.30 A/B/C/D 2-115 [» ]
    2VUD X-ray 1.70 A/B/C/D 2-115 [» ]
    3DCQ X-ray 1.80 A/B/C/D 2-115 [» ]
    3ZDV X-ray 1.41 A/B/C/D 1-115 [» ]
    ProteinModelPortali Q9HYN5.
    SMRi Q9HYN5. Positions 2-115.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA3361.

    Protocols and materials databases

    DNASUi 882528.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG06749 ; AAG06749 ; PA3361 .
    GeneIDi 882528.
    KEGGi pae:PA3361.
    PATRICi 19841323. VBIPseAer58763_3520.

    Organism-specific databases

    PseudoCAPi PA3361.

    Phylogenomic databases

    HOGENOMi HOG000110720.
    OMAi NDYNDGI.
    OrthoDBi EOG67X1RS.

    Miscellaneous databases

    EvolutionaryTracei Q9HYN5.

    Family and domain databases

    Gene3Di 2.60.120.400. 1 hit.
    InterProi IPR010907. Ca-mediated_lectin.
    IPR016927. Lectin_sugar-bd.
    [Graphical view ]
    Pfami PF07472. PA-IIL. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF029595. Lectin_LecB. 1 hit.
    SUPFAMi SSF82026. SSF82026. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228Imported.
    2. "Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients."
      Mitchell E., Houles C., Sudakevitz D., Wimmerova M., Gautier C., Perez S., Wu A.M., Gilboa-Garber N., Imberty A.
      Nat. Struct. Biol. 9:918-921(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) IN COMPLEX WITH CALCIUM AND FUCOSE.
    3. "Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa."
      Loris R., Tielker D., Jaeger K.E., Wyns L.
      J. Mol. Biol. 331:861-870(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.20 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM; FUCOSE AND MANNOSE.
    4. "Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa."
      Perret S., Sabin C., Dumon C., Pokorna M., Gautier C., Galanina O., Ilia S., Bovin N., Nicaise M., Desmadril M., Gilboa-Garber N., Wimmerova M., Mitchell E.P., Imberty A.
      Biochem. J. 389:325-332(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS) IN COMPLEX WITH CALCIUM; FUCOSE; GALACTOSE AND N-ACETYL-D-GLUCOSAMINE.
    5. "High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 A resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches."
      Mitchell E.P., Sabin C., Snajdrova L., Pokorna M., Perret S., Gautier C., Hofr C., Gilboa-Garber N., Koca J., Wimmerova M., Imberty A.
      Proteins 58:735-746(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.00 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM AND FUCOSE.
    6. "Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: thermodynamics data correlated with X-ray structures."
      Sabin C., Mitchell E.P., Pokorna M., Gautier C., Utille J.P., Wimmerova M., Imberty A.
      FEBS Lett. 580:982-987(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM.
    7. "Engineering of PA-IIL lectin from Pseudomonas aeruginosa - Unravelling the role of the specificity loop for sugar preference."
      Adam J., Pokorna M., Sabin C., Mitchell E.P., Imberty A., Wimmerova M.
      BMC Struct. Biol. 7:36-36(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) IN COMPLEX WITH CALCIUM AND FUCOSE.
    8. "X-ray structures and thermodynamics of the interaction of PA-IIL from Pseudomonas aeruginosa with disaccharide derivatives."
      Marotte K., Sabin C., Preville C., Moume-Pymbock M., Wimmerova M., Mitchell E.P., Imberty A., Roy R.
      ChemMedChem 2:1328-1338(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS) IN COMPLEX WITH CALCIUM; FUCOSE AND N-ACETYL-D-GLUCOSAMINE.
    9. "Inhibition and dispersion of Pseudomonas aeruginosa biofilms by glycopeptide dendrimers targeting the fucose-specific lectin LecB."
      Johansson E.M., Crusz S.A., Kolomiets E., Buts L., Kadam R.U., Cacciarini M., Bartels K.M., Diggle S.P., Camara M., Williams P., Loris R., Nativi C., Rosenau F., Jaeger K.E., Darbre T., Reymond J.L.
      Chem. Biol. 15:1249-1257(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM.
    10. "Fucose-Derived Glycomimetics as High Affinity Ligands for Bacterial Lectin Pa-Iil from Pseudomonas Aeruginosa."
      Beha S., Marotte K., Sabin C., Mitchell E.P., Imberty A., Roy R.
      Submitted (MAY-2008) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM AND FUCOSE.
    11. "Discovery of two classes of potent glycomimetic inhibitors of Pseudomonas aeruginosa LecB with distinct binding modes."
      Hauck D., Joachim I., Frommeyer B., Varrot A., Philipp B., Moller H.M., Imberty A., Exner T.E., Titz A.
      ACS Chem. Biol. 8:1775-1784(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.41 ANGSTROMS) IN COMPLEX WITH CALCIUM.

    Entry informationi

    Entry nameiQ9HYN5_PSEAE
    AccessioniPrimary (citable) accession number: Q9HYN5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 1, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3