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Protein
Submitted name:

Fucose-binding lectin PA-IIL

Gene

lecB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi22 – 221Calcium 1; via carbonyl oxygenCombined sources
Binding sitei24 – 241N-acetyl-D-glucosamineCombined sources
Metal bindingi96 – 961Calcium 2Combined sources
Binding sitei97 – 971N-acetyl-D-glucosamineCombined sources
Metal bindingi100 – 1001Calcium 2Combined sources
Binding sitei100 – 1001GalactoseCombined sources
Metal bindingi102 – 1021Calcium 1Combined sources
Metal bindingi102 – 1021Calcium 2Combined sources
Metal bindingi104 – 1041Calcium 1Combined sources
Metal bindingi105 – 1051Calcium 1Combined sources
Metal bindingi105 – 1051Calcium 2Combined sources
Metal bindingi115 – 1151Calcium 1; via carbonyl oxygenCombined sources
Metal bindingi115 – 1151Calcium 2; via carbonyl oxygenCombined sources
Binding sitei115 – 1151Fucose; via amide nitrogen and carbonyl oxygenCombined sources
Binding sitei115 – 1151Mannose; via amide nitrogen and carbonyl oxygenCombined sources

GO - Molecular functioni

  1. carbohydrate binding Source: PseudoCAP
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

CalciumCombined sources, LectinImported, Metal-bindingCombined sources

Names & Taxonomyi

Protein namesi
Submitted name:
Fucose-binding lectin PA-IILImported
Gene namesi
Name:lecBImported
Ordered Locus Names:PA3361Imported
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)Imported
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA3361.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3361.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GZTX-ray1.30A/B/C/D1-115[»]
1OURX-ray1.42A2-115[»]
1OUSX-ray1.20A/B/C/D2-115[»]
1OUXX-ray2.00A/B/C/D2-115[»]
1OVPX-ray1.40A2-115[»]
1OVSX-ray1.75A/B/C/D2-115[»]
1OXCX-ray1.20A/B/C/D2-115[»]
1UZVX-ray1.00A/B/C/D2-115[»]
1W8FX-ray1.05A/B/C/D1-115[»]
1W8HX-ray1.75A/B/C/D1-115[»]
2BOJX-ray1.80A/B/C/D2-115[»]
2BP6X-ray1.50A/B/C/D2-115[»]
2JDHX-ray1.10A/B/C/D1-115[»]
2JDKX-ray1.10A/B/C/D1-115[»]
2JDMX-ray1.70A/B/C/D1-115[»]
2JDNX-ray1.30A/B/C/D1-115[»]
2JDPX-ray1.30A/B/C/D1-115[»]
2JDUX-ray1.50A/B/C/D1-115[»]
2JDYX-ray1.70A/B/C/D1-115[»]
2VUCX-ray1.30A/B/C/D2-115[»]
2VUDX-ray1.70A/B/C/D2-115[»]
3DCQX-ray1.80A/B/C/D2-115[»]
3ZDVX-ray1.41A/B/C/D1-115[»]
4CE8X-ray0.90A/B/C/D2-115[»]
SMRiQ9HYN5. Positions 2-115.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HYN5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 243Fucose bindingCombined sources
Regioni22 – 243Mannose bindingCombined sources
Regioni97 – 1059Fucose bindingCombined sources
Regioni97 – 1059Mannose bindingCombined sources

Phylogenomic databases

HOGENOMiHOG000110720.
OMAiNDYNDGI.
OrthoDBiEOG67X1RS.

Family and domain databases

Gene3Di2.60.120.400. 1 hit.
InterProiIPR010907. Ca-mediated_lectin.
IPR016927. Lectin_sugar-bd.
[Graphical view]
PfamiPF07472. PA-IIL. 1 hit.
[Graphical view]
PIRSFiPIRSF029595. Lectin_LecB. 1 hit.
SUPFAMiSSF82026. SSF82026. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HYN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATQGVFTLP ANTRFGVTAF ANSSGTQTVN VLVNNETAAT FSGQSTNNAV
60 70 80 90 100
IGTQVLNSGS SGKVQVQVSV NGRPSDLVSA QVILTNELNF ALVGSEDGTD
110
NDYNDAVVVI NWPLG
Length:115
Mass (Da):11,863
Last modified:March 1, 2001 - v1
Checksum:i616D8249512CDD7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06749.1.
PIRiH83225.
RefSeqiNP_252051.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06749; AAG06749; PA3361.
GeneIDi882528.
KEGGipae:PA3361.
PATRICi19841323. VBIPseAer58763_3520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06749.1.
PIRiH83225.
RefSeqiNP_252051.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GZTX-ray1.30A/B/C/D1-115[»]
1OURX-ray1.42A2-115[»]
1OUSX-ray1.20A/B/C/D2-115[»]
1OUXX-ray2.00A/B/C/D2-115[»]
1OVPX-ray1.40A2-115[»]
1OVSX-ray1.75A/B/C/D2-115[»]
1OXCX-ray1.20A/B/C/D2-115[»]
1UZVX-ray1.00A/B/C/D2-115[»]
1W8FX-ray1.05A/B/C/D1-115[»]
1W8HX-ray1.75A/B/C/D1-115[»]
2BOJX-ray1.80A/B/C/D2-115[»]
2BP6X-ray1.50A/B/C/D2-115[»]
2JDHX-ray1.10A/B/C/D1-115[»]
2JDKX-ray1.10A/B/C/D1-115[»]
2JDMX-ray1.70A/B/C/D1-115[»]
2JDNX-ray1.30A/B/C/D1-115[»]
2JDPX-ray1.30A/B/C/D1-115[»]
2JDUX-ray1.50A/B/C/D1-115[»]
2JDYX-ray1.70A/B/C/D1-115[»]
2VUCX-ray1.30A/B/C/D2-115[»]
2VUDX-ray1.70A/B/C/D2-115[»]
3DCQX-ray1.80A/B/C/D2-115[»]
3ZDVX-ray1.41A/B/C/D1-115[»]
4CE8X-ray0.90A/B/C/D2-115[»]
SMRiQ9HYN5. Positions 2-115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3361.

Chemistry

BindingDBiQ9HYN5.

Protocols and materials databases

DNASUi882528.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06749; AAG06749; PA3361.
GeneIDi882528.
KEGGipae:PA3361.
PATRICi19841323. VBIPseAer58763_3520.

Organism-specific databases

PseudoCAPiPA3361.

Phylogenomic databases

HOGENOMiHOG000110720.
OMAiNDYNDGI.
OrthoDBiEOG67X1RS.

Miscellaneous databases

EvolutionaryTraceiQ9HYN5.

Family and domain databases

Gene3Di2.60.120.400. 1 hit.
InterProiIPR010907. Ca-mediated_lectin.
IPR016927. Lectin_sugar-bd.
[Graphical view]
PfamiPF07472. PA-IIL. 1 hit.
[Graphical view]
PIRSFiPIRSF029595. Lectin_LecB. 1 hit.
SUPFAMiSSF82026. SSF82026. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228Imported.
  2. "Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients."
    Mitchell E., Houles C., Sudakevitz D., Wimmerova M., Gautier C., Perez S., Wu A.M., Gilboa-Garber N., Imberty A.
    Nat. Struct. Biol. 9:918-921(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) IN COMPLEX WITH CALCIUM AND FUCOSE.
  3. "Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa."
    Loris R., Tielker D., Jaeger K.E., Wyns L.
    J. Mol. Biol. 331:861-870(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.20 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM; FUCOSE AND MANNOSE.
  4. "Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa."
    Perret S., Sabin C., Dumon C., Pokorna M., Gautier C., Galanina O., Ilia S., Bovin N., Nicaise M., Desmadril M., Gilboa-Garber N., Wimmerova M., Mitchell E.P., Imberty A.
    Biochem. J. 389:325-332(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS) IN COMPLEX WITH CALCIUM; FUCOSE; GALACTOSE AND N-ACETYL-D-GLUCOSAMINE.
  5. "High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 A resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches."
    Mitchell E.P., Sabin C., Snajdrova L., Pokorna M., Perret S., Gautier C., Hofr C., Gilboa-Garber N., Koca J., Wimmerova M., Imberty A.
    Proteins 58:735-746(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.00 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM AND FUCOSE.
  6. "Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: thermodynamics data correlated with X-ray structures."
    Sabin C., Mitchell E.P., Pokorna M., Gautier C., Utille J.P., Wimmerova M., Imberty A.
    FEBS Lett. 580:982-987(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM.
  7. "Engineering of PA-IIL lectin from Pseudomonas aeruginosa - Unravelling the role of the specificity loop for sugar preference."
    Adam J., Pokorna M., Sabin C., Mitchell E.P., Imberty A., Wimmerova M.
    BMC Struct. Biol. 7:36-36(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) IN COMPLEX WITH CALCIUM AND FUCOSE.
  8. "X-ray structures and thermodynamics of the interaction of PA-IIL from Pseudomonas aeruginosa with disaccharide derivatives."
    Marotte K., Sabin C., Preville C., Moume-Pymbock M., Wimmerova M., Mitchell E.P., Imberty A., Roy R.
    ChemMedChem 2:1328-1338(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS) IN COMPLEX WITH CALCIUM; FUCOSE AND N-ACETYL-D-GLUCOSAMINE.
  9. "Inhibition and dispersion of Pseudomonas aeruginosa biofilms by glycopeptide dendrimers targeting the fucose-specific lectin LecB."
    Johansson E.M., Crusz S.A., Kolomiets E., Buts L., Kadam R.U., Cacciarini M., Bartels K.M., Diggle S.P., Camara M., Williams P., Loris R., Nativi C., Rosenau F., Jaeger K.E., Darbre T., Reymond J.L.
    Chem. Biol. 15:1249-1257(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM.
  10. "Fucose-Derived Glycomimetics as High Affinity Ligands for Bacterial Lectin Pa-Iil from Pseudomonas Aeruginosa."
    Beha S., Marotte K., Sabin C., Mitchell E.P., Imberty A., Roy R.
    Submitted (MAY-2008) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM AND FUCOSE.
  11. "Discovery of two classes of potent glycomimetic inhibitors of Pseudomonas aeruginosa LecB with distinct binding modes."
    Hauck D., Joachim I., Frommeyer B., Varrot A., Philipp B., Moller H.M., Imberty A., Exner T.E., Titz A.
    ACS Chem. Biol. 8:1775-1784(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.41 ANGSTROMS) IN COMPLEX WITH CALCIUM.
  12. "Perdeuterated Pseudomonas Aeruginosa Lectin II Complex with Hydrogenated L Fucose and Calcium."
    Mossou E., Cuypers M.G., Mitchell E.P., Russi S., Pokorna M., Wimmerova M., Imberty A., Mcsweeney S., Haertlein M., Forsyth V.T.
    Submitted (NOV-2013) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (0.90 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM AND FUCOSE.

Entry informationi

Entry nameiQ9HYN5_PSEAE
AccessioniPrimary (citable) accession number: Q9HYN5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2001
Last sequence update: March 1, 2001
Last modified: March 4, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.