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Q9HYN5

- Q9HYN5_PSEAE

UniProt

Q9HYN5 - Q9HYN5_PSEAE

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Protein
Submitted name:

Fucose-binding lectin PA-IIL

Gene
lecB, PA3361
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Unreviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi22 – 221Calcium 1; via carbonyl oxygenImported
Binding sitei24 – 241N-acetyl-D-glucosamineImported
Metal bindingi96 – 961Calcium 2Imported
Binding sitei97 – 971N-acetyl-D-glucosamineImported
Metal bindingi100 – 1001Calcium 2Imported
Binding sitei100 – 1001GalactoseImported
Metal bindingi102 – 1021Calcium 1Imported
Metal bindingi102 – 1021Calcium 2Imported
Metal bindingi104 – 1041Calcium 1Imported
Metal bindingi105 – 1051Calcium 1Imported
Metal bindingi105 – 1051Calcium 2Imported
Metal bindingi115 – 1151Calcium 1; via carbonyl oxygenImported
Metal bindingi115 – 1151Calcium 2; via carbonyl oxygenImported
Binding sitei115 – 1151Fucose; via amide nitrogen and carbonyl oxygenImported
Binding sitei115 – 1151Mannose; via amide nitrogen and carbonyl oxygenImported

GO - Molecular functioni

  1. carbohydrate binding Source: PseudoCAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. biofilm formation Source: PseudoCAP
Complete GO annotation...

Keywords - Ligandi

CalciumImported, LectinImported, Metal-bindingImported

Names & Taxonomyi

Protein namesi
Submitted name:
Fucose-binding lectin PA-IILImported
Gene namesi
Name:lecBImported
Ordered Locus Names:PA3361Imported
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)Imported
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA3361.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3361.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GZTX-ray1.30A/B/C/D1-115[»]
1OURX-ray1.42A2-115[»]
1OUSX-ray1.20A/B/C/D2-115[»]
1OUXX-ray2.00A/B/C/D2-115[»]
1OVPX-ray1.40A2-115[»]
1OVSX-ray1.75A/B/C/D2-115[»]
1OXCX-ray1.20A/B/C/D2-115[»]
1UZVX-ray1.00A/B/C/D2-115[»]
1W8FX-ray1.05A/B/C/D1-115[»]
1W8HX-ray1.75A/B/C/D1-115[»]
2BOJX-ray1.80A/B/C/D2-115[»]
2BP6X-ray1.50A/B/C/D2-115[»]
2JDHX-ray1.10A/B/C/D1-115[»]
2JDKX-ray1.10A/B/C/D1-115[»]
2JDMX-ray1.70A/B/C/D1-115[»]
2JDNX-ray1.30A/B/C/D1-115[»]
2JDPX-ray1.30A/B/C/D1-115[»]
2JDUX-ray1.50A/B/C/D1-115[»]
2JDYX-ray1.70A/B/C/D1-115[»]
2VUCX-ray1.30A/B/C/D2-115[»]
2VUDX-ray1.70A/B/C/D2-115[»]
3DCQX-ray1.80A/B/C/D2-115[»]
3ZDVX-ray1.41A/B/C/D1-115[»]
ProteinModelPortaliQ9HYN5.
SMRiQ9HYN5. Positions 2-115.

Miscellaneous databases

EvolutionaryTraceiQ9HYN5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 243Fucose bindingImported
Regioni22 – 243Mannose bindingImported
Regioni97 – 1059Fucose bindingImported
Regioni97 – 1059Mannose bindingImported

Phylogenomic databases

HOGENOMiHOG000110720.
OMAiNDYNDGI.
OrthoDBiEOG67X1RS.

Family and domain databases

Gene3Di2.60.120.400. 1 hit.
InterProiIPR010907. Ca-mediated_lectin.
IPR016927. Lectin_sugar-bd.
[Graphical view]
PfamiPF07472. PA-IIL. 1 hit.
[Graphical view]
PIRSFiPIRSF029595. Lectin_LecB. 1 hit.
SUPFAMiSSF82026. SSF82026. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HYN5-1 [UniParc]FASTAAdd to Basket

« Hide

MATQGVFTLP ANTRFGVTAF ANSSGTQTVN VLVNNETAAT FSGQSTNNAV    50
IGTQVLNSGS SGKVQVQVSV NGRPSDLVSA QVILTNELNF ALVGSEDGTD 100
NDYNDAVVVI NWPLG 115
Length:115
Mass (Da):11,863
Last modified:March 1, 2001 - v1
Checksum:i616D8249512CDD7D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG06749.1.
PIRiH83225.
RefSeqiNP_252051.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06749; AAG06749; PA3361.
GeneIDi882528.
KEGGipae:PA3361.
PATRICi19841323. VBIPseAer58763_3520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG06749.1 .
PIRi H83225.
RefSeqi NP_252051.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GZT X-ray 1.30 A/B/C/D 1-115 [» ]
1OUR X-ray 1.42 A 2-115 [» ]
1OUS X-ray 1.20 A/B/C/D 2-115 [» ]
1OUX X-ray 2.00 A/B/C/D 2-115 [» ]
1OVP X-ray 1.40 A 2-115 [» ]
1OVS X-ray 1.75 A/B/C/D 2-115 [» ]
1OXC X-ray 1.20 A/B/C/D 2-115 [» ]
1UZV X-ray 1.00 A/B/C/D 2-115 [» ]
1W8F X-ray 1.05 A/B/C/D 1-115 [» ]
1W8H X-ray 1.75 A/B/C/D 1-115 [» ]
2BOJ X-ray 1.80 A/B/C/D 2-115 [» ]
2BP6 X-ray 1.50 A/B/C/D 2-115 [» ]
2JDH X-ray 1.10 A/B/C/D 1-115 [» ]
2JDK X-ray 1.10 A/B/C/D 1-115 [» ]
2JDM X-ray 1.70 A/B/C/D 1-115 [» ]
2JDN X-ray 1.30 A/B/C/D 1-115 [» ]
2JDP X-ray 1.30 A/B/C/D 1-115 [» ]
2JDU X-ray 1.50 A/B/C/D 1-115 [» ]
2JDY X-ray 1.70 A/B/C/D 1-115 [» ]
2VUC X-ray 1.30 A/B/C/D 2-115 [» ]
2VUD X-ray 1.70 A/B/C/D 2-115 [» ]
3DCQ X-ray 1.80 A/B/C/D 2-115 [» ]
3ZDV X-ray 1.41 A/B/C/D 1-115 [» ]
ProteinModelPortali Q9HYN5.
SMRi Q9HYN5. Positions 2-115.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA3361.

Protocols and materials databases

DNASUi 882528.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG06749 ; AAG06749 ; PA3361 .
GeneIDi 882528.
KEGGi pae:PA3361.
PATRICi 19841323. VBIPseAer58763_3520.

Organism-specific databases

PseudoCAPi PA3361.

Phylogenomic databases

HOGENOMi HOG000110720.
OMAi NDYNDGI.
OrthoDBi EOG67X1RS.

Miscellaneous databases

EvolutionaryTracei Q9HYN5.

Family and domain databases

Gene3Di 2.60.120.400. 1 hit.
InterProi IPR010907. Ca-mediated_lectin.
IPR016927. Lectin_sugar-bd.
[Graphical view ]
Pfami PF07472. PA-IIL. 1 hit.
[Graphical view ]
PIRSFi PIRSF029595. Lectin_LecB. 1 hit.
SUPFAMi SSF82026. SSF82026. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. "Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients."
    Mitchell E., Houles C., Sudakevitz D., Wimmerova M., Gautier C., Perez S., Wu A.M., Gilboa-Garber N., Imberty A.
    Nat. Struct. Biol. 9:918-921(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) IN COMPLEX WITH CALCIUM AND FUCOSE.
  3. "Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa."
    Loris R., Tielker D., Jaeger K.E., Wyns L.
    J. Mol. Biol. 331:861-870(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.20 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM; FUCOSE AND MANNOSE.
  4. "Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa."
    Perret S., Sabin C., Dumon C., Pokorna M., Gautier C., Galanina O., Ilia S., Bovin N., Nicaise M., Desmadril M., Gilboa-Garber N., Wimmerova M., Mitchell E.P., Imberty A.
    Biochem. J. 389:325-332(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS) IN COMPLEX WITH CALCIUM; FUCOSE; GALACTOSE AND N-ACETYL-D-GLUCOSAMINE.
  5. "High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 A resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches."
    Mitchell E.P., Sabin C., Snajdrova L., Pokorna M., Perret S., Gautier C., Hofr C., Gilboa-Garber N., Koca J., Wimmerova M., Imberty A.
    Proteins 58:735-746(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.00 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM AND FUCOSE.
  6. "Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: thermodynamics data correlated with X-ray structures."
    Sabin C., Mitchell E.P., Pokorna M., Gautier C., Utille J.P., Wimmerova M., Imberty A.
    FEBS Lett. 580:982-987(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM.
  7. "Engineering of PA-IIL lectin from Pseudomonas aeruginosa - Unravelling the role of the specificity loop for sugar preference."
    Adam J., Pokorna M., Sabin C., Mitchell E.P., Imberty A., Wimmerova M.
    BMC Struct. Biol. 7:36-36(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) IN COMPLEX WITH CALCIUM AND FUCOSE.
  8. "X-ray structures and thermodynamics of the interaction of PA-IIL from Pseudomonas aeruginosa with disaccharide derivatives."
    Marotte K., Sabin C., Preville C., Moume-Pymbock M., Wimmerova M., Mitchell E.P., Imberty A., Roy R.
    ChemMedChem 2:1328-1338(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS) IN COMPLEX WITH CALCIUM; FUCOSE AND N-ACETYL-D-GLUCOSAMINE.
  9. "Inhibition and dispersion of Pseudomonas aeruginosa biofilms by glycopeptide dendrimers targeting the fucose-specific lectin LecB."
    Johansson E.M., Crusz S.A., Kolomiets E., Buts L., Kadam R.U., Cacciarini M., Bartels K.M., Diggle S.P., Camara M., Williams P., Loris R., Nativi C., Rosenau F., Jaeger K.E., Darbre T., Reymond J.L.
    Chem. Biol. 15:1249-1257(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM.
  10. "Fucose-Derived Glycomimetics as High Affinity Ligands for Bacterial Lectin Pa-Iil from Pseudomonas Aeruginosa."
    Beha S., Marotte K., Sabin C., Mitchell E.P., Imberty A., Roy R.
    Submitted (MAY-2008) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) OF 2-115 IN COMPLEX WITH CALCIUM AND FUCOSE.
  11. "Discovery of two classes of potent glycomimetic inhibitors of Pseudomonas aeruginosa LecB with distinct binding modes."
    Hauck D., Joachim I., Frommeyer B., Varrot A., Philipp B., Moller H.M., Imberty A., Exner T.E., Titz A.
    ACS Chem. Biol. 8:1775-1784(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.41 ANGSTROMS) IN COMPLEX WITH CALCIUM.

Entry informationi

Entry nameiQ9HYN5_PSEAE
AccessioniPrimary (citable) accession number: Q9HYN5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2001
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteome, Reference proteome

External Data

Dasty 3

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