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Reviewed, UniProtKB/Swiss-Prot Q9HYL2 (NOSZ_PSEAE)

Last modified November 3, 2009. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Nitrous-oxide reductase
    EC=1.7.99.6
Alternative name(s):
    N(2)OR
    N2O reductase
Gene names
Name: nosZ
Ordered Locus Names: PA3392
OrganismPseudomonas aeruginosa [Complete proteome] [HAMAP]
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

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Protein attributes

Sequence length636 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide By similarity.

Catalytic activity

N2 + H2O + acceptor = N2O + reduced acceptor. HAMAP MF_00716

Cofactor

Binds 2 calcium ions per subunit By similarity.

Binds 6 copper ions per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 4/4. HAMAP MF_00716

Subunit structure

Homodimer By similarity.

Subcellular location

Periplasm By similarity.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_00716

Sequence similarities

Belongs to the nosZ family.

In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

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Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   LigandCalcium
Copper
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentmembrane

Inferred from electronic annotation. Source: InterPro

periplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: HAMAP

copper ion binding

Inferred from electronic annotation. Source: HAMAP

cytochrome-c oxidase activity

Inferred from electronic annotation. Source: InterPro

nitrous-oxide reductase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4949Tat-type signal Potential
Chain50 – 636587Nitrous-oxide reductase HAMAP MF_00716
PRO_0000019828

Regions

Region540 – 63697COX2-like HAMAP MF_00716

Sites

Metal binding1271Copper Z2 By similarity
Metal binding1281Copper Z3 By similarity
Metal binding1761Copper Z2 By similarity
Metal binding2541Calcium 2; via carbonyl oxygen By similarity
Metal binding2571Calcium 2 By similarity
Metal binding2651Calcium 2; via carbonyl oxygen By similarity
Metal binding2711Calcium 2 By similarity
Metal binding3221Calcium 2 By similarity
Metal binding3241Copper Z1 By similarity
Metal binding3801Copper Z1 By similarity
Metal binding4311Copper Z3 By similarity
Metal binding4521Calcium 1; via carbonyl oxygen By similarity
Metal binding4671Calcium 1 By similarity
Metal binding4921Copper Z4 By similarity
Metal binding5811Copper A1 By similarity
Metal binding6161Copper A1 By similarity
Metal binding6161Copper A2 By similarity
Metal binding6181Copper A2; via carbonyl oxygen By similarity
Metal binding6201Copper A1 By similarity
Metal binding6201Copper A2 By similarity
Metal binding6241Copper A2 By similarity
Metal binding6271Copper A1 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9HYL2-1 [UniParc].

Last modified January 11, 2001. Version 1.
Checksum: F3D66D519CF220BF

FASTA63670,660
        10         20         30         40         50         60 
MSDDTKSPHE ETHGLNRRGF LGASALTGAA ALVGASALGS AVVGREARAA GKGERSKAEV 

        70         80         90        100        110        120 
APGELDEYYG FWSGGHSGEV RVLGVPSMRE LMRIPVFNVD SATGWGLTNE SKRVLGDSAR 

       130        140        150        160        170        180 
FLNGDCHHPH ISMTDGKYDG KYLFINDKAN SRVARIRLDV MKCDRIVTIP NVQAIHGLRL 

       190        200        210        220        230        240 
QKVPHTRYVF CNAEFIIPHP NDGSTFDLSG DNAFTLYNAI DAETMEVAWQ VIVDGNLDNT 

       250        260        270        280        290        300 
DMDYSGRFAA STCYNSEKAV DLGGMMRNER DWVVVFDIPR IEAEIKAKRF VTLGDSKVPV 

       310        320        330        340        350        360 
VDGRRKDGKD SPVTRYIPVP KNPHGLNTSP DGKYFIANGK LSPTCTMIAI ERLGDLFAGK 

       370        380        390        400        410        420 
LADPRDVVVG EPELGLGPLH TTFDGRGNAY TTLFIDSQLV KWNLADAVRA YKGEKVDYIR 

       430        440        450        460        470        480 
QKLDVQYQPG HNHATLCETS EADGKWIVVL SKFSKDRFLP TGPLHPENDQ LIDISGEEMK 

       490        500        510        520        530        540 
LVHDGPTFAE PHDCILARRD QIKTRKIWDR KDPFFAETVK RAEKDGIDLM KDNKVIREGN 

       550        560        570        580        590        600 
KVRVYMVSMA PSFGLTEFKV KQGDEVTVTI TNLDEIEDVT HGFVMVNHGV CMEISPQQTS 

       610        620        630 
SITFVADKPG VHWYYCSWFC HALHMEMCGR MLVEKA

« Hide

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Cross-references

Sequence databases

AE004091 Genomic DNA. Translation: AAG06780.1.
PIRC83222.
RefSeqNP_252082.1.

3D structure databases

HSSPHSSP built from PDB template 1FWX based on UniProtKB Q51705.
ModBaseSearch...

Genome annotation databases

GeneID879824.
GenomeReviewsGene locus PA3392 in contig AE004091_GR.
KEGGpae:PA3392.
NMPDRfig|208964.1.peg.3392.

Organism-specific databases

PseudoCAPPA3392.
CMRSearch...

Phylogenomic databases

HOGENOMQ9HYL2.
OMASKDRFLP.

Enzyme and pathway databases

BioCycPAER208964:PA3392-MON.
BRENDA1.7.99.6. 354.

Family and domain databases

HAMAPMF_00716.
[Tree]
InterProIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR002429. Cyt_c_oxidase_su2_C.
IPR006311. TAT_signal.
IPR017909. Twin_arg_translocation_Tat.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
Gene3DG3DSA:2.60.40.420. Cupredoxin. 1 hit.
G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
PfamPF00116. COX2. 1 hit.
[Graphical view]
TIGRFAMsTIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNOSZ_PSEAE
AccessionPrimary (citable) accession number: Q9HYL2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents