Reviewed, UniProtKB/Swiss-Prot Q9HYG2 (SSUD_PSEAE)

Names and origin

Protein names

Recommended name:
    Alkanesulfonate monooxygenase
    EC=1.14.14.5
Alternative name(s):
    FMNH2-dependent aliphatic sulfonate monooxygenase
    PA13

Gene names

Name:	ssuD
Ordered Locus Names:	PA3444

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the desulfonation of aliphatic sulfonates By similarity.
Catalytic activity	An alkanesufonate (R-CH(2)-SO3H) + FMNH2 + O2 = an aldehyde (R-CHO) + FMN + sulfite + H2O. HAMAP MF_01229
Induction	Repressed by sulfate, cysteine, or thiocyanate. HAMAP MF_01229
Miscellaneous	FMNH2 which is absolutely required for this enzymatic reaction, is provided by ssuE By similarity.
Sequence similarities	Belongs to the ssuD family.

Ontologies

Keywords
Ligand	FMN
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanesulfonate monooxygenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 382	381	Alkanesulfonate monooxygenase HAMAP MF_01229		PRO_0000216711
Experimental info
	Sequence conflict	7	1	W → A AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9HYG2-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: BF2C6EE4A1E102CD	FASTA	382	41,567
	10 20 30 40 50 60 MSLEIFWFLP THGDGHYLGT TQGARAVDHG YLQQIAQAAD RLGFGGVLIP TGRSCEDSWL 70 80 90 100 110 120 VAASLIPVTQ RLKFLVALRP GIISPTVAAR QAATLDRLSN GRALFNLVTG GDPDELAGDG 130 140 150 160 170 180 LHLSHAERYE ASVEFTRIWR RVLEGETVDY AGKHIQVKGA KLLYPPLQQP RPPLYFGGSS 190 200 210 220 230 240 EAAQDLAAEQ VELYLTWGEP PAAVAEKIAQ VREKAARQGR QVRFGIRLHV IVRETSEEAW 250 260 270 280 290 300 QAADRLIAHL DDDTIARAQA SLARFDSVGQ QRMAALHGGS RDNLEVSPNL WAGVGLVRGG 310 320 330 340 350 360 AGTALVGDGP TVAARVREYA ELGIDTFIFS GYPHLEESYR VAELLFPHLD VQRPAQPEGR 370 380 GYVSPFGEMV ANDILPRQAA QS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Regulation of the sulfate starvation response in Pseudomonas aeruginosa: role of cysteine biosynthetic intermediates." Hummerjohann J., Kuttel E., Quadroni M., Ragaller J., Leisinger T., Kertesz M.A. Microbiology 144:1375-1386(1998) [PubMed: 9611812] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-14. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG06832.1.
PIR	A83215.
RefSeq	NP_252134.1.
3D structure databases
HSSP	HSSP built from PDB template 1M41 based on UniProtKB P80645.
SMR	Q9HYG2. Positions 1-358.
ModBase	Search...
Genome annotation databases
GeneID	879163.
GenomeReviews	Gene locus PA3444 in contig AE004091_GR.
KEGG	pae:PA3444.
Organism-specific databases
PseudoCAP	PA3444.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HYG2.
OMA	Q9HYG2. NIFWFLP.
Enzyme and pathway databases
BioCyc	PAER208964:PA3444-MON.
BRENDA	1.14.14.5. 354.
Family and domain databases
HAMAP	MF_01229. [Tree]
InterPro	IPR019911. Alkanesulfonate_mOase_FMN-dep. IPR016048. Luciferase-like_sub. [Graphical view]
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03565. Alk_sulf_monoox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SSUD_PSEAE

Accession

Primary (citable) accession number: Q9HYG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2003
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 43 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)