Reviewed,
UniProtKB/Swiss-Prot Q9HYD5 (MAO1_PSEAE)
Last modified
June 16, 2009.
Version 47.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: NAD-dependent malic enzyme Short name=NAD-ME EC=1.1.1.38 | ||||
| Gene names |
| ||||
| Organism | Pseudomonas aeruginosa [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 287 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas |
Protein attributes
| Sequence length | 564 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | (S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619 |
| Cofactor | Divalent metal cations. Prefers magnesium or manganese By similarity. |
| Subunit structure | Homotetramer By similarity. |
| Sequence similarities | Belongs to the malic enzymes family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activityInferred from electronic annotation. Source: HAMAP metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 564 | 564 | NAD-dependent malic enzyme HAMAP MF_01619 | PRO_0000160224 | |||||
Sites | |||||||||
| Active site | 102 | 1 | Proton donor By similarity | ||||||
| Active site | 173 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 244 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 245 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 268 | 1 | Divalent metal cation By similarity | ||||||
| Binding site | 155 | 1 | NAD By similarity | ||||||
| Binding site | 268 | 1 | NAD By similarity | ||||||
| Binding site | 417 | 1 | NAD By similarity | ||||||
| Site | 268 | 1 | Important for activity By similarity | ||||||
Sequences
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References
| [1] | "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R.  Olson M.V.Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228. |
Cross-references
Sequence databases | |
|---|---|
| AE004091 Genomic DNA. Translation: AAG06859.1. | |
| PIR | D83211. |
| RefSeq | NP_252161.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1O0S based on UniProtKB P27443. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 879925. |
| GenomeReviews | Gene locus PA3471 in contig AE004091_GR. |
| KEGG | pae:PA3471. |
| NMPDR | fig|208964.1.peg.3471. |
Organism-specific databases | |
| PseudoCAP | PA3471. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q9HYD5. |
| OMA | Q9HYD5. IQDNNET. |
Enzyme and pathway databases | |
| BioCyc | PAER208964:PA3471-MON. |
| BRENDA | 1.1.1.38. 354. |
Family and domain databases | |
| HAMAP | MF_01619. [Tree] |
| InterPro | IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| Pfam | PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view] |
| PRINTS | PR00072. MALOXRDTASE. |
| PROSITE | PS00331. MALIC_ENZYMES. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MAO1_PSEAE | ||||||||
| Accession | Primary (citable) accession number: Q9HYD5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
