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Protein

Probable L-aspartate dehydrogenase

Gene

nadX

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.UniRule annotation

Miscellaneous

The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.UniRule annotation

Catalytic activityi

L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes iminoaspartate from L-aspartate (dehydrogenase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Probable L-aspartate dehydrogenase (nadX)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes iminoaspartate from L-aspartate (dehydrogenase route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei124NAD; via amide nitrogenUniRule annotation1
Binding sitei190NADUniRule annotation1
Active sitei220UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyridine nucleotide biosynthesis
LigandNAD, NADP

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-3573-MONOMER
BRENDAi1.4.1.21 5087
UniPathwayiUPA00253; UER00456

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L-aspartate dehydrogenaseUniRule annotation (EC:1.4.1.21UniRule annotation)
Gene namesi
Name:nadXUniRule annotation
Ordered Locus Names:PA3505
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3505

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001448901 – 267Probable L-aspartate dehydrogenaseAdd BLAST267

Proteomic databases

PaxDbiQ9HYA4
PRIDEiQ9HYA4

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3505

Structurei

3D structure databases

ProteinModelPortaliQ9HYA4
SMRiQ9HYA4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the L-aspartate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108Q27 Bacteria
COG1712 LUCA
HOGENOMiHOG000206326
InParanoidiQ9HYA4
KOiK06989
OMAiMIMSVGA

Family and domain databases

HAMAPiMF_01265 NadX, 1 hit
InterProiView protein in InterPro
IPR005106 Asp/hSer_DH_NAD-bd
IPR002811 Asp_DH
IPR020626 Asp_DH_prok
IPR011182 L-Asp_DH
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01958 DUF108, 1 hit
PF03447 NAD_binding_3, 1 hit
PIRSFiPIRSF005227 Asp_dh_NAD_syn, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HYA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNIVMIGCG AIGAGVLELL ENDPQLRVDA VIVPRDSETQ VRHRLASLRR
60 70 80 90 100
PPRVLSALPA GERPDLLVEC AGHRAIEQHV LPALAQGIPC LVVSVGALSE
110 120 130 140 150
PGLVERLEAA AQAGGSRIEL LPGAIGAIDA LSAARVGGLE SVRYTGRKPA
160 170 180 190 200
SAWLGTPGET VCDLQRLEKA RVIFDGSARE AARLYPKNAN VAATLSLAGL
210 220 230 240 250
GLDRTQVRLI ADPESCENVH QVEASGAFGG FELTLRGKPL AANPKTSALT
260
VYSVVRALGN HAHAISI
Length:267
Mass (Da):27,920
Last modified:March 1, 2001 - v1
Checksum:i208FBC975A872F86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG06893.1
PIRiG83206
RefSeqiNP_252195.1, NC_002516.2
WP_003112905.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG06893; AAG06893; PA3505
GeneIDi879175
KEGGipae:PA3505
PATRICifig|208964.12.peg.3669

Similar proteinsi

Entry informationi

Entry nameiASPD_PSEAE
AccessioniPrimary (citable) accession number: Q9HYA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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