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Protein

Ribonuclease T

Gene

rnt

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit. The active form of the enzyme binds two Mg2+ ions in its active site. The first Mg2+ forms only one salt bridge with the protein.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi35 – 351Magnesium 1; catalyticUniRule annotation1 Publication
Metal bindingi35 – 351Magnesium 2; catalyticUniRule annotation
Metal bindingi37 – 371Magnesium 2; catalyticUniRule annotation
Sitei41 – 411Important for substrate binding and specificityUniRule annotation
Sitei89 – 891Important for substrate binding and specificityUniRule annotation
Sitei136 – 1361Important for substrate binding and specificityUniRule annotation
Sitei158 – 1581Important for substrate binding and specificityUniRule annotation
Active sitei193 – 1931Proton donor/acceptorUniRule annotation
Metal bindingi193 – 1931Magnesium 2; catalyticUniRule annotation
Metal bindingi198 – 1981Magnesium 2; catalyticUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.13.3. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease TUniRule annotation (EC:3.1.13.-UniRule annotation)
Alternative name(s):
Exoribonuclease TUniRule annotation
Short name:
RNase TUniRule annotation
Gene namesi
Name:rntUniRule annotation
Ordered Locus Names:PA3528
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3528.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Ribonuclease TPRO_0000208969Add
BLAST

Proteomic databases

PaxDbiQ9HY82.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

DIPiDIP-29430N.
STRINGi208964.PA3528.

Structurei

Secondary structure

1
224
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 255Combined sources
Turni26 – 283Combined sources
Beta strandi29 – 4113Combined sources
Turni43 – 453Combined sources
Beta strandi48 – 5811Combined sources
Beta strandi64 – 7411Combined sources
Helixi84 – 907Combined sources
Helixi104 – 12118Combined sources
Beta strandi125 – 1339Combined sources
Helixi134 – 14815Combined sources
Beta strandi155 – 1628Combined sources
Helixi163 – 1719Combined sources
Helixi176 – 1827Combined sources
Helixi195 – 21521Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2F96X-ray2.09A/B1-224[»]
ProteinModelPortaliQ9HY82.
SMRiQ9HY82. Positions 19-220.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HY82.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 206175ExonucleaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RNase T family.UniRule annotation
Contains 1 exonuclease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DH5. Bacteria.
COG0847. LUCA.
HOGENOMiHOG000279358.
InParanoidiQ9HY82.
KOiK03683.
OMAiCYMVNHL.
PhylomeDBiQ9HY82.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00157. RNase_T. 1 hit.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR005987. RNase_T.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR01298. RNaseT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HY82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEDNFDDEF DGSLPSGPRH PMARRFRGYL PVVVDVETGG FNSATDALLE
60 70 80 90 100
IAATTVGMDE KGFLFPEHTY FFRIEPFEGA NIEPAALEFT GIKLDHPLRM
110 120 130 140 150
AVQEEAALTE IFRGIRKALK ANGCKRAILV GHNSSFDLGF LNAAVARTGI
160 170 180 190 200
KRNPFHPFSS FDTATLAGLA YGQTVLAKAC QAAGMEFDNR EAHSARYDTE
210 220
KTAELFCGIV NRWKEMGGWM DDDD
Length:224
Mass (Da):24,723
Last modified:March 1, 2001 - v1
Checksum:iCC1010D3AD60F442
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06916.1.
PIRiF83204.
RefSeqiNP_252218.1. NC_002516.2.
WP_003092070.1. NZ_ASJY01000572.1.

Genome annotation databases

EnsemblBacteriaiAAG06916; AAG06916; PA3528.
GeneIDi879810.
KEGGipae:PA3528.
PATRICi19841669. VBIPseAer58763_3692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06916.1.
PIRiF83204.
RefSeqiNP_252218.1. NC_002516.2.
WP_003092070.1. NZ_ASJY01000572.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2F96X-ray2.09A/B1-224[»]
ProteinModelPortaliQ9HY82.
SMRiQ9HY82. Positions 19-220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29430N.
STRINGi208964.PA3528.

Proteomic databases

PaxDbiQ9HY82.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06916; AAG06916; PA3528.
GeneIDi879810.
KEGGipae:PA3528.
PATRICi19841669. VBIPseAer58763_3692.

Organism-specific databases

PseudoCAPiPA3528.

Phylogenomic databases

eggNOGiENOG4105DH5. Bacteria.
COG0847. LUCA.
HOGENOMiHOG000279358.
InParanoidiQ9HY82.
KOiK03683.
OMAiCYMVNHL.
PhylomeDBiQ9HY82.

Enzyme and pathway databases

BRENDAi3.1.13.3. 5087.

Miscellaneous databases

EvolutionaryTraceiQ9HY82.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00157. RNase_T. 1 hit.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR005987. RNase_T.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR01298. RNaseT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNT_PSEAE
AccessioniPrimary (citable) accession number: Q9HY82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Member of the DEDD group of RNAses that are characterized by the presence of four acidic residues in the active site. These residues are conserved even when the proteins have highly divergent sequences.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.