Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribonuclease T

Gene

rnt

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit. The active form of the enzyme binds two Mg2+ ions in its active site. The first Mg2+ forms only one salt bridge with the protein.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi35Magnesium 1; catalyticUniRule annotation1 Publication1
Metal bindingi35Magnesium 2; catalyticUniRule annotation1
Metal bindingi37Magnesium 2; catalyticUniRule annotation1
Sitei41Important for substrate binding and specificityUniRule annotation1
Sitei89Important for substrate binding and specificityUniRule annotation1
Sitei136Important for substrate binding and specificityUniRule annotation1
Sitei158Important for substrate binding and specificityUniRule annotation1
Active sitei193Proton donor/acceptorUniRule annotation1
Metal bindingi193Magnesium 2; catalyticUniRule annotation1
Metal bindingi198Magnesium 2; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.13.3. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease TUniRule annotation (EC:3.1.13.-UniRule annotation)
Alternative name(s):
Exoribonuclease TUniRule annotation
Short name:
RNase TUniRule annotation
Gene namesi
Name:rntUniRule annotation
Ordered Locus Names:PA3528
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3528.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002089691 – 224Ribonuclease TAdd BLAST224

Proteomic databases

PaxDbiQ9HY82.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

DIPiDIP-29430N.
STRINGi208964.PA3528.

Structurei

Secondary structure

1224
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 25Combined sources5
Turni26 – 28Combined sources3
Beta strandi29 – 41Combined sources13
Turni43 – 45Combined sources3
Beta strandi48 – 58Combined sources11
Beta strandi64 – 74Combined sources11
Helixi84 – 90Combined sources7
Helixi104 – 121Combined sources18
Beta strandi125 – 133Combined sources9
Helixi134 – 148Combined sources15
Beta strandi155 – 162Combined sources8
Helixi163 – 171Combined sources9
Helixi176 – 182Combined sources7
Helixi195 – 215Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2F96X-ray2.09A/B1-224[»]
ProteinModelPortaliQ9HY82.
SMRiQ9HY82.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HY82.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 206ExonucleaseUniRule annotationAdd BLAST175

Sequence similaritiesi

Belongs to the RNase T family.UniRule annotation
Contains 1 exonuclease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DH5. Bacteria.
COG0847. LUCA.
HOGENOMiHOG000279358.
InParanoidiQ9HY82.
KOiK03683.
OMAiCYMVNHL.
PhylomeDBiQ9HY82.

Family and domain databases

CDDicd06134. RNaseT. 1 hit.
Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00157. RNase_T. 1 hit.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR005987. RNase_T.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR01298. RNaseT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HY82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEDNFDDEF DGSLPSGPRH PMARRFRGYL PVVVDVETGG FNSATDALLE
60 70 80 90 100
IAATTVGMDE KGFLFPEHTY FFRIEPFEGA NIEPAALEFT GIKLDHPLRM
110 120 130 140 150
AVQEEAALTE IFRGIRKALK ANGCKRAILV GHNSSFDLGF LNAAVARTGI
160 170 180 190 200
KRNPFHPFSS FDTATLAGLA YGQTVLAKAC QAAGMEFDNR EAHSARYDTE
210 220
KTAELFCGIV NRWKEMGGWM DDDD
Length:224
Mass (Da):24,723
Last modified:March 1, 2001 - v1
Checksum:iCC1010D3AD60F442
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06916.1.
PIRiF83204.
RefSeqiNP_252218.1. NC_002516.2.
WP_003092070.1. NZ_ASJY01000572.1.

Genome annotation databases

EnsemblBacteriaiAAG06916; AAG06916; PA3528.
GeneIDi879810.
KEGGipae:PA3528.
PATRICi19841669. VBIPseAer58763_3692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06916.1.
PIRiF83204.
RefSeqiNP_252218.1. NC_002516.2.
WP_003092070.1. NZ_ASJY01000572.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2F96X-ray2.09A/B1-224[»]
ProteinModelPortaliQ9HY82.
SMRiQ9HY82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29430N.
STRINGi208964.PA3528.

Proteomic databases

PaxDbiQ9HY82.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06916; AAG06916; PA3528.
GeneIDi879810.
KEGGipae:PA3528.
PATRICi19841669. VBIPseAer58763_3692.

Organism-specific databases

PseudoCAPiPA3528.

Phylogenomic databases

eggNOGiENOG4105DH5. Bacteria.
COG0847. LUCA.
HOGENOMiHOG000279358.
InParanoidiQ9HY82.
KOiK03683.
OMAiCYMVNHL.
PhylomeDBiQ9HY82.

Enzyme and pathway databases

BRENDAi3.1.13.3. 5087.

Miscellaneous databases

EvolutionaryTraceiQ9HY82.

Family and domain databases

CDDicd06134. RNaseT. 1 hit.
Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00157. RNase_T. 1 hit.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR005987. RNase_T.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR01298. RNaseT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNT_PSEAE
AccessioniPrimary (citable) accession number: Q9HY82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Member of the DEDD group of RNAses that are characterized by the presence of four acidic residues in the active site. These residues are conserved even when the proteins have highly divergent sequences.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.