Reviewed, UniProtKB/Swiss-Prot Q9HY41 (GLPK2_PSEAE)

Names and origin

Protein names

Recommended name:
    Glycerol kinase 2
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase 2
    Glycerokinase 2
      Short name=GK 2

Gene names

Name:	glpK2
Ordered Locus Names:	PA3579

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Glycerol kinase 2 HAMAP MF_00186		PRO_0000059477
Regions
	Nucleotide binding	408 – 412	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	308	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HY41-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 8DA2621FFF540582	FASTA	494	54,208
	10 20 30 40 50 60 MSHYLLAIDQ GTTSSRAIVF SAQGLPVASC QQEFKQYFPK DGWVEHDGEE IWLTTLQVCR 70 80 90 100 110 120 DALARKGLRA ADIAAIGITN QRETTLVWDA ASGDLIHPAI VWQDRRTADY CAELKAAGHE 130 140 150 160 170 180 ANVSARTGLL IDPYFSATKL RWILDNVPGA RQRAERGELR FGTVDCFLLW RLTGGRSHRT 190 200 210 220 230 240 DATNASRTLL FNIHSQDWDE ELLALFEIPR SLLPEVLDCA AEFGVSEPSL LGAAIPVLGM 250 260 270 280 290 300 AGDQQAALIG QACFQPGMVK STYGTGCFMI QNTGEQPVTS KNRLLTTVGY RLDGKVSYAV 310 320 330 340 350 360 EGSIFVAGAA VQWLRDGIKL IDHAHESEAL AIQAGDSNGV YLVPAFTGLG APYWDPKARG 370 380 390 400 410 420 AIFGLTRDTG IKEIVTAGLQ SVCYQTRDLL EAMRQDGTPP SALRVDGGMV VNNWMMQFLT 430 440 450 460 470 480 DILGVTVERP EVTETTALGV AYMAGLKAGF YRDLDDIASH WHLQRRFAAH MAEERRGELY 490 AGWQNAVRRV RSEA

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG06967.1.
PIR	F83199.
RefSeq	NP_252269.1.
3D structure databases
SMR	Q9HY41. Positions 3-492.
ModBase	Search...
Genome annotation databases
GeneID	880178.
GenomeReviews	Gene locus PA3579 in contig AE004091_GR.
KEGG	pae:PA3579.
NMPDR	fig|208964.1.peg.3579.
Organism-specific databases
PseudoCAP	PA3579.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	MEMLPEV.
Enzyme and pathway databases
BioCyc	PAER208964:PA3579-MONOMER.
BRENDA	2.7.1.30. 354.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK2_PSEAE

Accession

Primary (citable) accession number: Q9HY41

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)