Q9HY23 (KDGL_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase
Short name=DAGK
EC=2.7.1.107

Alternative name(s):
Diglyceride kinase
Short name=DGK

Gene names

Name:	dgkA
Ordered Locus Names:	PA3603

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	123 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Recycling of diacylglycerol produced during the turnover of membrane phospholipid By similarity.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the bacterial diacylglycerol kinase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 123	123	Diacylglycerol kinase		PRO_0000287766
Regions
	Topological domain	1 – 34	34	Cytoplasmic Potential
	Transmembrane	35 – 55	21	Helical; Potential
	Transmembrane	61 – 81	21	Helical; Potential
	Topological domain	82 – 101	20	Cytoplasmic Potential
	Transmembrane	102 – 122	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9HY23 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 1B8FE001E1EAE9A6	FASTA	123	13,138
	10 20 30 40 50 60 MSPSPFKGQT GLKRILNATG YSLAGFLAAF RGEAAFRQLV LLNVVLIPVA FLLDVSRGER 70 80 90 100 110 120 ALMIAVCLLA LIVELLNSAI EATVDRVSLE RHPLSKNAKD MGSAAQFVAL TVITVTWATI LLG

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG06991.1.
PIR	E83196.
RefSeq	NP_252293.1. NC_002516.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	880149.
GenomeReviews	Gene locus PA3603 in contig AE004091_GR.
KEGG	pae:PA3603.
PATRIC	19841825. VBIPseAer58763_3770.
Organism-specific databases
PseudoCAP	PA3603.
Phylogenomic databases
HOGENOM	HBG729542.
OMA	IRFAIDE.
ProtClustDB	CLSK868105.
Enzyme and pathway databases
BioCyc	PAER208964:PA3603-MONOMER.
Family and domain databases
InterPro	IPR000829. Diacylglycerol_kinase_prok. [Graphical view]
KO	K00901.
Pfam	PF01219. DAGK_prokar. 1 hit. [Graphical view]
PROSITE	PS01069. DAGK_PROKAR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDGL_PSEAE

Accession

Primary (citable) accession number: Q9HY23

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program