ID   LPXB_PSEAE              Reviewed;         378 AA.
AC   Q9HXY8;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   27-MAR-2024, entry version 111.
DE   RecName: Full=Lipid-A-disaccharide synthase;
DE            EC=2.4.1.182;
GN   Name=lpxB; OrderedLocusNames=PA3643;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 /
RC   1C / PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Condensation of UDP-2,3-diacylglucosamine and 2,3-
CC       diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor
CC       of lipid A, a phosphorylated glycolipid that anchors the
CC       lipopolysaccharide to the outer membrane of the cell. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a lipid X + a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-
CC         glucosamine = a lipid A disaccharide + H(+) + UDP;
CC         Xref=Rhea:RHEA:67828, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:137748, ChEBI:CHEBI:176338, ChEBI:CHEBI:176343;
CC         EC=2.4.1.182;
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.
CC   -!- SIMILARITY: Belongs to the LpxB family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG07031.1; -; Genomic_DNA.
DR   PIR; C83190; C83190.
DR   RefSeq; NP_252333.1; NC_002516.2.
DR   RefSeq; WP_003113865.1; NZ_QZGE01000001.1.
DR   AlphaFoldDB; Q9HXY8; -.
DR   SMR; Q9HXY8; -.
DR   STRING; 208964.PA3643; -.
DR   CAZy; GT19; Glycosyltransferase Family 19.
DR   PaxDb; 208964-PA3643; -.
DR   GeneID; 880521; -.
DR   KEGG; pae:PA3643; -.
DR   PATRIC; fig|208964.12.peg.3812; -.
DR   PseudoCAP; PA3643; -.
DR   HOGENOM; CLU_036577_3_0_6; -.
DR   InParanoid; Q9HXY8; -.
DR   OrthoDB; 9801642at2; -.
DR   PhylomeDB; Q9HXY8; -.
DR   BioCyc; PAER208964:G1FZ6-3713-MONOMER; -.
DR   UniPathway; UPA00973; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0008915; F:lipid-A-disaccharide synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005543; F:phospholipid binding; IBA:GO_Central.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR   HAMAP; MF_00392; LpxB; 1.
DR   InterPro; IPR003835; Glyco_trans_19.
DR   NCBIfam; TIGR00215; lpxB; 1.
DR   PANTHER; PTHR30372; LIPID-A-DISACCHARIDE SYNTHASE; 1.
DR   PANTHER; PTHR30372:SF7; LIPID-A-DISACCHARIDE SYNTHASE; 1.
DR   Pfam; PF02684; LpxB; 1.
DR   SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Lipid A biosynthesis; Lipid biosynthesis;
KW   Lipid metabolism; Reference proteome; Transferase.
FT   CHAIN           1..378
FT                   /note="Lipid-A-disaccharide synthase"
FT                   /id="PRO_0000190177"
SQ   SEQUENCE   378 AA;  41294 MW;  46DB015E89C9874D CRC64;
     MADGLRVALV AGEASGDILG SGLMQALRAR HPDIEFIGVG GPRMEAEGLS SYFPMERLSV
     MGLVEVLGRL PELLRRRKRL IRTLIEARPD VMIGIDAPDF TLGVEHKLRQ AGLRTVHYVS
     PSVWAWRQKR VLKIREACDL MLALFPFEAR FYEEHGVPVR FVGHPLANTI PLQADRAAAR
     ARLGLPADGQ VLALMPGSRG GEVGKLGALF LDTAQRLLVE RPGLRFVLPC ASAARREQIE
     QMLQGREPLP LTLLDGASHE ALAACDAVLI ASGTATLEAL LYKRPMVVAY RVAGLTYRIL
     KRLVKSPYIS LPNLLAGRLL VPELIQDAAT PQALAATLSP LLDDGSQQVE FFDAIHRALR
     QDASAQAAEA VLQLVERR
//