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Protein

UDP-3-O-acylglucosamine N-acyltransferase

Gene

lpxD

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein].UniRule annotation

Pathwayi: LPS lipid A biosynthesis

This protein is involved in the pathway LPS lipid A biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway LPS lipid A biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei242 – 2421Proton acceptorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-3-O-acylglucosamine N-acyltransferaseUniRule annotation (EC:2.3.1.-UniRule annotation)
Gene namesi
Name:lpxDUniRule annotation
Ordered Locus Names:PA3646
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3646.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353UDP-3-O-acylglucosamine N-acyltransferasePRO_0000059690Add
BLAST

Proteomic databases

PaxDbiQ9HXY6.

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA3646.

Structurei

Secondary structure

1
353
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87Combined sources
Helixi9 – 168Combined sources
Beta strandi19 – 224Combined sources
Beta strandi27 – 326Combined sources
Helixi34 – 363Combined sources
Beta strandi41 – 444Combined sources
Helixi48 – 569Combined sources
Beta strandi60 – 645Combined sources
Helixi66 – 694Combined sources
Beta strandi76 – 783Combined sources
Helixi82 – 909Combined sources
Helixi91 – 933Combined sources
Beta strandi101 – 1033Combined sources
Beta strandi167 – 1693Combined sources
Beta strandi180 – 1834Combined sources
Beta strandi188 – 1914Combined sources
Beta strandi194 – 1974Combined sources
Beta strandi204 – 2063Combined sources
Beta strandi217 – 2193Combined sources
Beta strandi222 – 2243Combined sources
Beta strandi226 – 2283Combined sources
Beta strandi284 – 2885Combined sources
Beta strandi302 – 3043Combined sources
Helixi313 – 32412Combined sources
Helixi326 – 33914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PMOX-ray1.30A2-353[»]
ProteinModelPortaliQ9HXY6.
SMRiQ9HXY6. Positions 2-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transferase hexapeptide repeat family. LpxD subfamily.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105D7M. Bacteria.
COG1044. LUCA.
HOGENOMiHOG000294339.
InParanoidiQ9HXY6.
KOiK02536.
OMAiWANVTLY.
OrthoDBiEOG6JB11D.
PhylomeDBiQ9HXY6.

Family and domain databases

HAMAPiMF_00523. LpxD.
InterProiIPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR007691. LpxD.
IPR011004. Trimer_LpxA-like.
IPR020573. UDP_GlcNAc_AcTrfase_non-rep.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF04613. LpxD. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01853. lipid_A_lpxD. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HXY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSTLSYTLG QLAAHVGAEV RGDADLPIQG LATLQEAGPA QLSFLANPQY
60 70 80 90 100
RKYLPESRAG AVLLTAADAD GFAGTALVVA NPYLAYASLS HLFDRKPKAA
110 120 130 140 150
AGIHPTAIVA ADAEVDPSAS VGAYAVIESG ARIGAGVSIG AHCVIGARSV
160 170 180 190 200
IGEGGWLAPR VTLYHDVTIG ARVSIQSGAV IGGEGFGFAN EKGVWQKIAQ
210 220 230 240 250
IGGVTIGDDV EIGANTTIDR GALSDTLIGN GVKLDNQIMI AHNVQIGDHT
260 270 280 290 300
AMAACVGISG SAKIGRHCML AGGVGLVGHI EICDNVFVTG MTMVTRSITE
310 320 330 340 350
PGSYSSGTAM QPAAEWKKSA ARIRQLDDMA RRLQQLEKRL AAVTSSGDAS

SDA
Length:353
Mass (Da):36,191
Last modified:March 1, 2001 - v1
Checksum:i4B6C4D329F63EA35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07034.1.
PIRiF83190.
RefSeqiNP_252336.1. NC_002516.2.
WP_003098585.1. NZ_ASJY01000591.1.

Genome annotation databases

EnsemblBacteriaiAAG07034; AAG07034; PA3646.
GeneIDi880525.
KEGGipae:PA3646.
PATRICi19841915. VBIPseAer58763_3815.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07034.1.
PIRiF83190.
RefSeqiNP_252336.1. NC_002516.2.
WP_003098585.1. NZ_ASJY01000591.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PMOX-ray1.30A2-353[»]
ProteinModelPortaliQ9HXY6.
SMRiQ9HXY6. Positions 2-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3646.

Proteomic databases

PaxDbiQ9HXY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07034; AAG07034; PA3646.
GeneIDi880525.
KEGGipae:PA3646.
PATRICi19841915. VBIPseAer58763_3815.

Organism-specific databases

PseudoCAPiPA3646.

Phylogenomic databases

eggNOGiENOG4105D7M. Bacteria.
COG1044. LUCA.
HOGENOMiHOG000294339.
InParanoidiQ9HXY6.
KOiK02536.
OMAiWANVTLY.
OrthoDBiEOG6JB11D.
PhylomeDBiQ9HXY6.

Enzyme and pathway databases

UniPathwayiUPA00973.

Family and domain databases

HAMAPiMF_00523. LpxD.
InterProiIPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR007691. LpxD.
IPR011004. Trimer_LpxA-like.
IPR020573. UDP_GlcNAc_AcTrfase_non-rep.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF04613. LpxD. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01853. lipid_A_lpxD. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. "The structure of LpxD from Pseudomonas aeruginosa at 1.3 A resolution."
    Badger J., Chie-Leon B., Logan C., Sridhar V., Sankaran B., Zwart P.H., Nienaber V.
    Acta Crystallogr. F 67:749-752(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS).

Entry informationi

Entry nameiLPXD_PSEAE
AccessioniPrimary (citable) accession number: Q9HXY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: March 1, 2001
Last modified: November 11, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.