Reviewed, UniProtKB/Swiss-Prot Q9HXW7 (PLSB_PSEAE)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	PA3673

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 834	834	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_0000195228
Regions
	Motif	310 – 315	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q9HXW7-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C668FB7242B3681D	FASTA	834	94,816
	10 20 30 40 50 60 MPRYPFRRFG FGALRRLLYL WVRSETINQS AFTLKIDRSK PVLYVLQQPS VSDLAVVDTE 70 80 90 100 110 120 CRKAGLPRPV MPVAVGDAIE PAAFFYLTPE PDWLGRQDKR GASPTLVRML AAVGQNGLDD 130 140 150 160 170 180 AQIIPVSVFW GQSPDSESSP WKLLFADNWA VTGRLRKLAR ILILGRKTRV QFSAPIHLRE 190 200 210 220 230 240 LVEQGKGHER TLRMVNRILR VHFRNLKTAV IGPDLSHRRN LVKGLLRAPL VRQAISEECE 250 260 270 280 290 300 SERISQEKAE GIALRYANEI ASDFSYPVIR FLEVILSWFW NKLYEGVKVN HIERVQDVAQ 310 320 330 340 350 360 GNEIVYVPCH RSHIDYLLLS YLLFRNGLTP PHIAAGINLN MPVIGSILRR GGAFFMRRSF 370 380 390 400 410 420 KGNQLYTAVF NEYLHTLFSR GFSTEYFVEG GRSRTGRMLH PRTGMLAITL RSFLRDSRRP 430 440 450 460 470 480 IVFVPVYIGY ERVLEGRTYL GELRGATKKK ESIFDLFKVV GALKQRFGQV WVNFGEPIHL 490 500 510 520 530 540 DQFLDRHQPD WQDQDLGPEY RPDWLPQTTN LLAKDVARHL NDAAAINPVN LVALALLSTS 550 560 570 580 590 600 RQALDESALA RILDLYLALL RKVPYSPSAT LPDGDGQALI EYVKSMNLLA EQKDALGRIL 610 620 630 640 650 660 YLDEQNAVLA TYYRNNVLHV FALPALIASF FQSNSRISRE QLLRFARALY PYLQAELFIR 670 680 690 700 710 720 WSLDELDAVI DQWLAALVEQ DLLRQENDTF IRPAPSSRQY VLLILLARSV TQTLQRFYMA 730 740 750 760 770 780 IALLLNAGQN ALTAEELENL CTVMAQRLSI LHGLNAPEFF DKSLFRHFIQ TLLDLRVLRK 790 800 810 820 830 DEAGKLSYHE LLGELAEGAA KRVLPAEIRL SIRQVALERP AEEAAAESND AAAN

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG07061.1.
PIR	F83185.
RefSeq	NP_252363.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	880591.
GenomeReviews	Gene locus PA3673 in contig AE004091_GR.
KEGG	pae:PA3673.
Organism-specific databases
PseudoCAP	PA3673.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296590.
OMA	WNKLYQG.
Enzyme and pathway databases
BioCyc	PAER208964:PA3673-MONOMER.
BRENDA	2.3.1.15. 354.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_PSEAE

Accession

Primary (citable) accession number: Q9HXW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)