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Reviewed, UniProtKB/Swiss-Prot Q9HXN2 (PUR4_PSEAE)

Last modified February 9, 2010. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase
Gene names
Name: purL
Ordered Locus Names: PA3763
OrganismPseudomonas aeruginosa [Complete proteome] [HAMAP]
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

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Protein attributes

Sequence length1298 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer By similarity. HAMAP MF_00419

Subcellular location

Cytoplasm By similarity HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

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Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12981298Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000100413

Regions

Domain1045 – 1298254Glutamine amidotransferase type-1
Nucleotide binding305 – 31612ATP Potential

Sites

Active site11381Nucleophile By similarity
Active site12631 By similarity
Active site12651 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9HXN2-1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 95BBE47CCA4FE8D1

FASTA1,298140,647
        10         20         30         40         50         60 
MLILRGAPAL SAFRHGKLLE QLTQHVPAVT GLYAEFAHFA DVTGALTADE EQVLARLLKY 

        70         80         90        100        110        120 
GPSVPVQEPS GRLFLVVPRF GTISPWSSKA SDIARNCGLA KIDRLERGIA YYVQGELSES 

       130        140        150        160        170        180 
DAQQVAARLH DRMTQLVLDR LEGAAELFSH AQPRPLTAVD VLGGGRAALE KANVELGLAL 

       190        200        210        220        230        240 
AEDEIDYLLK SFGELGRNPH DVELMMFAQA NSEHCRHKIF NASWDIDGQA QDKSLFGMIK 

       250        260        270        280        290        300 
NTYEMNREGV LSAYKDNAAV IVGHVAGRFF PDPQTREYAA SREPVQILMK VETHNHPTAI 

       310        320        330        340        350        360 
APFPGASTGS GGEIRDEGAT GRGAKPKAGL TGFTVSNLQI PGFEQPWEVP YGKPERIVTA 

       370        380        390        400        410        420 
LDIMVEGPLG GAAFNNEFGR PALTGYFRTF EQKIATPHGE EVRGYHKPIM LAGGMGNIRD 

       430        440        450        460        470        480 
EHVQKGEISV GAKLIVLGGP AMLIGLGGGA ASSMATGASS ADLDFASVQR DNPEMERRCQ 

       490        500        510        520        530        540 
EVIDRCWQLG ERNPISFIHD VGAGGLSNAL PELINDGGRG GRFELRAVPN DEPGMSPLEI 

       550        560        570        580        590        600 
WCNESQERYV LSVDAADFET FKAICERERC PFAVVGEAIE QRQLTVADSH FDNKPVDMPL 

       610        620        630        640        650        660 
EVLLGKAPRM HRAVTREAEL GDDFDAAGLE LQESVERVLR HPAVASKSFL ITIGDRTITG 

       670        680        690        700        710        720 
LVARDQMVGP WQVPVADCAV TATSFDVYTG EAMAMGERTP LALLDAPASG RMAIGETVTN 

       730        740        750        760        770        780 
LAAARVGKLS DIKLSANWMA AAGHPGEDAR LYDTVKAVGM ELCPELGITI PVGKDSMSMK 

       790        800        810        820        830        840 
TRWQDNGEDK SVTSPVSLIV TGFAPVADVR QSLTPQLRLD KGETDLILID LGRGKNRLGG 

       850        860        870        880        890        900 
SILAQVHGKL GRAVPDVDDA EDLKAFFAVI QGLNADGHIL AYHDRSDGGL ITSVLEMAFA 

       910        920        930        940        950        960 
GHCGVELNLD ALADSREELA AVLFSEELGA VIQVREGATP EVLAQFSAAG LDDCVAVIGQ 

       970        980        990       1000       1010       1020 
PVNGYEINLN YNGETVYSAQ RRILQRIWSE TSYQIQRLRD NADCAEQEFD ALLDEDNPGL 

      1030       1040       1050       1060       1070       1080 
SIKLSYDVND DIAAPYIKKG VRPKVAILRE QGVNGQVEMA AAFDRAGFAA IDVHMSDILA 

      1090       1100       1110       1120       1130       1140 
GRVDLDAFKG LVACGGFSYG DVLGAGEGWA KSILFNARAR DGFQAFFARK DSFALGVCNG 

      1150       1160       1170       1180       1190       1200 
CQMMSNLHEL IPGTEFWPHF VRNRSEQFEA RVAMVQVQES SSIFLQGMAG SRLPIAIAHG 

      1210       1220       1230       1240       1250       1260 
EGHAEFESEE ALLEADLSGC VSLRFVDNHG KVTEAYPANP NGSPRGITGL SSRDGRVTIM 

      1270       1280       1290 
MPHPERVFRA VQNSWRPDDW QEDGGWLRMF RNARVWVD

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE004091 Genomic DNA. Translation: AAG07150.1.
PIRB83175.
RefSeqNP_252452.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID879752.
GenomeReviewsGene locus PA3763 in contig AE004091_GR.
KEGGpae:PA3763.
NMPDRfig|208964.1.peg.3762.

Organism-specific databases

PseudoCAPPA3763.
CMRSearch...

Phylogenomic databases

HOGENOMHBG335309.
OMAERGIAYY.

Enzyme and pathway databases

BioCycPAER208964:PA3763-MONOMER.
BRENDA6.3.5.3. 354.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM_N-like.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePUR4_PSEAE
AccessionPrimary (citable) accession number: Q9HXN2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: March 1, 2001
Last modified: February 9, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents