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Protein

Membrane-bound lytic murein transglycosylase F

Gene

mltF

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.UniRule annotation

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei316 – 3161UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound lytic murein transglycosylase FUniRule annotation (EC:4.2.2.n1UniRule annotation)
Alternative name(s):
Murein lyase FUniRule annotation
Gene namesi
Name:mltFUniRule annotation
Ordered Locus Names:PA3764
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3764.

Subcellular locationi

  • Cell outer membrane; Peripheral membrane protein

  • Note: Attached to the inner leaflet of the outer membrane.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232UniRule annotationAdd
BLAST
Chaini33 – 490458Membrane-bound lytic murein transglycosylase FPRO_0000353957Add
BLAST

Proteomic databases

PaxDbiQ9HXN1.

Structurei

Secondary structure

1
490
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 428Combined sources
Beta strandi44 – 529Combined sources
Turni53 – 553Combined sources
Beta strandi56 – 594Combined sources
Beta strandi62 – 643Combined sources
Helixi66 – 7813Combined sources
Beta strandi81 – 899Combined sources
Helixi90 – 978Combined sources
Beta strandi104 – 1063Combined sources
Beta strandi120 – 1223Combined sources
Beta strandi127 – 1293Combined sources
Beta strandi131 – 1366Combined sources
Helixi145 – 1484Combined sources
Beta strandi153 – 1564Combined sources
Helixi160 – 17112Combined sources
Beta strandi178 – 1825Combined sources
Helixi185 – 1939Combined sources
Beta strandi196 – 2038Combined sources
Helixi204 – 2107Combined sources
Turni211 – 2133Combined sources
Beta strandi217 – 2226Combined sources
Beta strandi227 – 2293Combined sources
Beta strandi232 – 2376Combined sources
Helixi240 – 25415Combined sources
Helixi257 – 26610Combined sources
Helixi268 – 2725Combined sources
Helixi274 – 28613Combined sources
Helixi288 – 30215Combined sources
Helixi306 – 31712Combined sources
Beta strandi326 – 3283Combined sources
Turni331 – 3344Combined sources
Helixi337 – 3437Combined sources
Helixi351 – 36818Combined sources
Helixi377 – 38812Combined sources
Helixi390 – 40213Combined sources
Helixi410 – 4167Combined sources
Helixi417 – 4215Combined sources
Helixi423 – 4264Combined sources
Beta strandi429 – 4313Combined sources
Helixi436 – 45419Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OWDX-ray2.21A33-459[»]
4OYVX-ray2.31A28-460[»]
4OZ9X-ray2.24A33-460[»]
4P0GX-ray1.65A28-460[»]
4P11X-ray1.89A28-460[»]
ProteinModelPortaliQ9HXN1.
SMRiQ9HXN1. Positions 32-460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 269237Non-LT domainUniRule annotationAdd
BLAST
Regioni270 – 490221LT domainUniRule annotationAdd
BLAST

Domaini

The N-terminal domain does not have lytic activity and probably modulates enzymatic activity. The C-terminal domain is the catalytic active domain.UniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the bacterial solute-binding protein 3 family.UniRule annotation
In the C-terminal section; belongs to the transglycosylase Slt family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CHQ. Bacteria.
COG4623. LUCA.
HOGENOMiHOG000218316.
InParanoidiQ9HXN1.
KOiK18691.
OMAiKYGYARG.

Family and domain databases

HAMAPiMF_02016. MltF. 1 hit.
InterProiIPR023346. Lysozyme-like_dom.
IPR023703. MltF.
IPR001638. Solute-binding_3/MltF_N.
IPR000189. Transglyc_AS.
IPR008258. Transglycosylase_SLT_dom_1.
[Graphical view]
PANTHERiPTHR21666:SF215. PTHR21666:SF215. 1 hit.
PfamiPF00497. SBP_bac_3. 1 hit.
PF01464. SLT. 1 hit.
[Graphical view]
SMARTiSM00062. PBPb. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 2 hits.
PROSITEiPS00922. TRANSGLYCOSYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9HXN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFALTAYRLR CAAWLLATGI FLLLAGCSEA KAPTALERVQ KEGVLRVITR
60 70 80 90 100
NSPATYFQDR NGETGFEYEL AKRFAERLGV ELKIETADNL DDLYAQLSRE
110 120 130 140 150
GGPALAAAGL TPGREDDASV RYSHTYLDVT PQIIYRNGQQ RPTRPEDLVG
160 170 180 190 200
KRIMVLKGSS HAEQLAELKK QYPELKYEES DAVEVVDLLR MVDVGDIDLT
210 220 230 240 250
LVDSNELAMN QVYFPNVRVA FDFGEARGLA WALPGGDDDS LMNEVNAFLD
260 270 280 290 300
QAKKEGLLQR LKDRYYGHVD VLGYVGAYTF AQHLQQRLPR YESHFKQSGK
310 320 330 340 350
QLDTDWRLLA AIGYQESLWQ PGATSKTGVR GLMMLTNRTA QAMGVSNRLD
360 370 380 390 400
PKQSIQGGSK YFVQIRSELP ESIKEPDRSW FALAAYNIGG AHLEDARKMA
410 420 430 440 450
EKEGLNPNKW LDVKKMLPRL AQKQWYAKTR YGYARGGETV HFVQNVRRYY
460 470 480 490
DILTWVTQPQ MEGSQIAESG LHLPGVNKTR PEEDSGDEKL
Length:490
Mass (Da):55,227
Last modified:November 25, 2008 - v2
Checksum:i191CB459DBB67121
GO

Sequence cautioni

The sequence AAG07151 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07151.1. Different initiation.
PIRiC83175.
RefSeqiNP_252453.1. NC_002516.2.
WP_003104806.1. NZ_ASJY01000606.1.

Genome annotation databases

EnsemblBacteriaiAAG07151; AAG07151; PA3764.
GeneIDi880530.
KEGGipae:PA3764.
PATRICi19842163. VBIPseAer58763_3939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07151.1. Different initiation.
PIRiC83175.
RefSeqiNP_252453.1. NC_002516.2.
WP_003104806.1. NZ_ASJY01000606.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OWDX-ray2.21A33-459[»]
4OYVX-ray2.31A28-460[»]
4OZ9X-ray2.24A33-460[»]
4P0GX-ray1.65A28-460[»]
4P11X-ray1.89A28-460[»]
ProteinModelPortaliQ9HXN1.
SMRiQ9HXN1. Positions 32-460.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.

Proteomic databases

PaxDbiQ9HXN1.

Protocols and materials databases

DNASUi880530.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07151; AAG07151; PA3764.
GeneIDi880530.
KEGGipae:PA3764.
PATRICi19842163. VBIPseAer58763_3939.

Organism-specific databases

PseudoCAPiPA3764.

Phylogenomic databases

eggNOGiENOG4105CHQ. Bacteria.
COG4623. LUCA.
HOGENOMiHOG000218316.
InParanoidiQ9HXN1.
KOiK18691.
OMAiKYGYARG.

Family and domain databases

HAMAPiMF_02016. MltF. 1 hit.
InterProiIPR023346. Lysozyme-like_dom.
IPR023703. MltF.
IPR001638. Solute-binding_3/MltF_N.
IPR000189. Transglyc_AS.
IPR008258. Transglycosylase_SLT_dom_1.
[Graphical view]
PANTHERiPTHR21666:SF215. PTHR21666:SF215. 1 hit.
PfamiPF00497. SBP_bac_3. 1 hit.
PF01464. SLT. 1 hit.
[Graphical view]
SMARTiSM00062. PBPb. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 2 hits.
PROSITEiPS00922. TRANSGLYCOSYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLTF_PSEAE
AccessioniPrimary (citable) accession number: Q9HXN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: September 7, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.