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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Pathwayi: XMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Inosine-5'-monophosphate dehydrogenase (guaB)
This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981ATP; via amide nitrogen and carbonyl oxygenCombined sources
Binding sitei153 – 1531ATPCombined sources
Binding sitei159 – 1591ATP; via amide nitrogen and carbonyl oxygenCombined sources
Active sitei304 – 3041Thioimidate intermediateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi118 – 1203ATPCombined sources
Nucleotide bindingi134 – 1374ATPCombined sources
Nucleotide bindingi180 – 1812ATPCombined sources
Nucleotide bindingi198 – 1992ATPCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

GMP biosynthesisUniRule annotation, Purine biosynthesis

Keywords - Ligandi

ATP-bindingCombined sources, Metal-bindingUniRule annotation, NADUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

BRENDAi1.1.1.205. 5087.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Gene namesi
Name:guaBImported
Ordered Locus Names:PA3770Imported
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)Imported
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3770.

PTM / Processingi

Proteomic databases

PaxDbiQ9HXM5.
PRIDEiQ9HXM5.

Interactioni

Protein-protein interaction databases

DIPiDIP-60162N.
STRINGi208964.PA3770.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZFHX-ray2.25A1-489[»]
4AVFX-ray2.23A/B/C/D1-489[»]
4DQWX-ray2.51A/B1-489[»]
5AHLX-ray1.95A1-489[»]
5AHMX-ray1.74A/B1-489[»]
5AHNX-ray1.65A1-489[»]
ProteinModelPortaliQ9HXM5.
SMRiQ9HXM5. Positions 1-487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini93 – 14856CBSInterPro annotationAdd
BLAST
Domaini152 – 21059CBSInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0516. LUCA.
COG0517. LUCA.
ENOG4105CP4. Bacteria.
HOGENOMiHOG000165755.
InParanoidiQ9HXM5.
KOiK00088.
OMAiSSMGYCG.
OrthoDBiEOG6GTZPV.
PhylomeDBiQ9HXM5.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 2 hits.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HXM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRISQEALT FDDVLLIPGY SEVLPKDVSL KTRLTRGIEL NIPLVSAAMD
60 70 80 90 100
TVTEARLAIA MAQEGGIGII HKNMGIEQQA AEVRKVKKHE TAIVRDPVTV
110 120 130 140 150
TPSTKIIELL QMAREYGFSG FPVVEQGELV GIVTGRDLRV KPNAGDTVAA
160 170 180 190 200
IMTPKDKLVT AREGTPLEEM KAKLYENRIE KMLVVDENFY LRGLVTFRDI
210 220 230 240 250
EKAKTYPLAS KDEQGRLRVG AAVGTGADTG ERVAALVAAG VDVVVVDTAH
260 270 280 290 300
GHSKGVIERV RWVKQTFPDV QVIGGNIATA EAAKALAEAG ADAVKVGIGP
310 320 330 340 350
GSICTTRIVA GVGVPQISAI ANVAAALEGT GVPLIADGGI RFSGDLAKAM
360 370 380 390 400
VAGAYCVMMG SMFAGTEEAP GEIELFQGRS YKSYRGMGSL GAMSGSQGSS
410 420 430 440 450
DRYFQDASAG AEKLVPEGIE GRVPYKGALS AIVHQLMGGL RAAMGYTGSA
460 470 480
DIQQMRTQPQ FVRITGAGMA ESHVHDVQIT KEAPNYRVG
Length:489
Mass (Da):51,707
Last modified:March 1, 2001 - v1
Checksum:i6D2320AFD52C888B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07157.1.
PIRiH83173.
RefSeqiNP_252459.1. NC_002516.2.
WP_003092702.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG07157; AAG07157; PA3770.
GeneIDi880535.
KEGGipae:PA3770.
PATRICi19842179. VBIPseAer58763_3947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07157.1.
PIRiH83173.
RefSeqiNP_252459.1. NC_002516.2.
WP_003092702.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZFHX-ray2.25A1-489[»]
4AVFX-ray2.23A/B/C/D1-489[»]
4DQWX-ray2.51A/B1-489[»]
5AHLX-ray1.95A1-489[»]
5AHMX-ray1.74A/B1-489[»]
5AHNX-ray1.65A1-489[»]
ProteinModelPortaliQ9HXM5.
SMRiQ9HXM5. Positions 1-487.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60162N.
STRINGi208964.PA3770.

Proteomic databases

PaxDbiQ9HXM5.
PRIDEiQ9HXM5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07157; AAG07157; PA3770.
GeneIDi880535.
KEGGipae:PA3770.
PATRICi19842179. VBIPseAer58763_3947.

Organism-specific databases

PseudoCAPiPA3770.

Phylogenomic databases

eggNOGiCOG0516. LUCA.
COG0517. LUCA.
ENOG4105CP4. Bacteria.
HOGENOMiHOG000165755.
InParanoidiQ9HXM5.
KOiK00088.
OMAiSSMGYCG.
OrthoDBiEOG6GTZPV.
PhylomeDBiQ9HXM5.

Enzyme and pathway databases

UniPathwayiUPA00601; UER00295.
BRENDAi1.1.1.205. 5087.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 2 hits.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery."
    Moynie L., Schnell R., McMahon S.A., Sandalova T., Boulkerou W.A., Schmidberger J.W., Alphey M., Cukier C., Duthie F., Kopec J., Liu H., Jacewicz A., Hunter W.N., Naismith J.H., Schneider G.
    Acta Crystallogr. F 69:25-34(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.23 ANGSTROMS).
  2. "Structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase."
    Rao V.A., Shepherd S.M., Owen R., Hunter W.N.
    Acta Crystallogr. F 69:243-247(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).
  3. Cited for: X-RAY CRYSTALLOGRAPHY (2.51 ANGSTROMS) IN COMPLEX WITH ATP.
  4. "Crystallographic studies of two variants of Pseudomonas aeruginosa IMPDH with impaired allosteric regulation."
    Labesse G., Alexandre T., Gelin M., Haouz A., Munier-Lehmann H.
    Acta Crystallogr. D 71:1890-1899(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

Entry informationi

Entry nameiQ9HXM5_PSEAE
AccessioniPrimary (citable) accession number: Q9HXM5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2001
Last sequence update: March 1, 2001
Last modified: December 9, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.