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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:PA3987
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3987.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Leucine--tRNA ligasePRO_0000152066Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3987.

Structurei

3D structure databases

ProteinModelPortaliQ9HX33.
SMRiQ9HX33. Positions 1-871.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi632 – 6365"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
InParanoidiQ9HX33.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiQ9HX33.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HX33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHEQYQPLEI ETQAQNYWKE HQSFLVRELP DKEKFYCLSM FPYPSGKLHM
60 70 80 90 100
GHVRNYTIGD VISRYHRMQG RNVLQPMGWD AFGMPAENAA MKNNVAPAAW
110 120 130 140 150
TYDNIAYMKS QLDSLGLAID WSREVTTCKP DYYRWEQWLF TRLFEKGVIY
160 170 180 190 200
RKNGTVNWDP VDQTVLANEQ VIDGRGWRSG ALIEKREIPM YYFKITAYAE
210 220 230 240 250
ELLESLDNLP GWPEQVKTMQ RNWIGKSRGM EIGFPYDQAS IGHAGQLKVF
260 270 280 290 300
TTRPDTLMGA TYVAVAAEHP LATQAAQNDP QLQAFIDECK RGGVAEADIA
310 320 330 340 350
TQEKKGMATS LFVEHPLTGD KLPVWVANYV LMNYGEGAVM AVPGHDERDF
360 370 380 390 400
EFANKYGLPI RQVIAKVEGD NDFESSVWKE WYGAKDESVL TVNSGKYDNL
410 420 430 440 450
GYQAAFDAIG ADLEAKGLGQ ARTQFRLRDW GISRQRYWGC PIPIIHCEAC
460 470 480 490 500
GDVPVPADQL PVVLPEDVVP DGSGSPLAKM PEFYECNCPK CGQPAKRETD
510 520 530 540 550
TMDTFVESSW YFARYACPQF EGGMLDRKAA DYWLPVDQYI GGIEHAILHL
560 570 580 590 600
LYARFFHKLM RDEGLVGSDE PFKNLLTQGM VVADTYYRTT ANGGKDWFNP
610 620 630 640 650
ADVEVERDAK AKVVGARLKS DGQPVEIGGT EKMSKSKNNG VDPQSMIDQY
660 670 680 690 700
GADTCRLFMM FASPPDMSLE WSDAGVEGAN RFLRRVWRLA HAHVSAGLPG
710 720 730 740 750
ALDVAALSDA QKQVRRAIHL AIRQASQDVG QHHKFNTAIA AVMTLMNVLE
760 770 780 790 800
KAPNQDAQDR ALIQEGLETV VLLLAPITPH ICHVLWGQLG HAEAVIDARW
810 820 830 840 850
PSVDESALVQ DTLQLVVQVN GKLRGHIDVA ASASREDVEA AARANENVLR
860 870
FTEGLSIRKV IVVPGKLVNI VAN
Length:873
Mass (Da):97,648
Last modified:March 1, 2001 - v1
Checksum:iA61D2FDD098E69DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07374.1.
PIRiE83148.
RefSeqiNP_252676.1. NC_002516.2.
WP_003100324.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG07374; AAG07374; PA3987.
GeneIDi878925.
KEGGipae:PA3987.
PATRICi19842647. VBIPseAer58763_4180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07374.1.
PIRiE83148.
RefSeqiNP_252676.1. NC_002516.2.
WP_003100324.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ9HX33.
SMRiQ9HX33. Positions 1-871.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3987.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07374; AAG07374; PA3987.
GeneIDi878925.
KEGGipae:PA3987.
PATRICi19842647. VBIPseAer58763_4180.

Organism-specific databases

PseudoCAPiPA3987.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
InParanoidiQ9HX33.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiQ9HX33.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiSYL_PSEAE
AccessioniPrimary (citable) accession number: Q9HX33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.