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Q9HX25

- LIPA_PSEAE

UniProt

Q9HX25 - LIPA_PSEAE

Protein

Lipoyl synthase

Gene

lipA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi100 – 1001Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi104 – 1041Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:PA3996
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA3996.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Lipoyl synthasePRO_0000102342Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA3996.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HX25.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiRKVDCDI.
    OrthoDBiEOG6038ZS.
    PhylomeDBiQ9HX25.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9HX25-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTVVEKSGE AKPGKVEVGV KLRGAEKVAR IPVKIIPTEE LPKKPDWIRV    50
    RIPVSPEVDR IKQLLRKHKL HSVCEEASCP NLGECFSGGT ATFMIMGDIC 100
    TRRCPFCDVG HGRPKPLDVD EPTNLAIAIA DLRLKYVVIT SVDRDDLRDG 150
    GAQHFADCLR EIRKLSPGIQ LETLVPDYRG RMDIALEITA NEPPDVFNHN 200
    LETVPRLYRS SRPGSDFEWS LDLLQKFKQM VPHVPTKSGL MLGLGETDDE 250
    VIEVMQRMRE HDIDMLTLGQ YLQPSRNHLP VQRFVHPDTF AWFAEEGEKM 300
    GFKNVASGPL VRSSYHADQQ AHGNKIG 327
    Length:327
    Mass (Da):36,733
    Last modified:March 1, 2001 - v1
    Checksum:i0A0C8BDD44681753
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG07383.1.
    PIRiH83145.
    RefSeqiNP_252685.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG07383; AAG07383; PA3996.
    GeneIDi878931.
    KEGGipae:PA3996.
    PATRICi19842663. VBIPseAer58763_4188.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG07383.1 .
    PIRi H83145.
    RefSeqi NP_252685.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali Q9HX25.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA3996.

    Protocols and materials databases

    DNASUi 878931.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG07383 ; AAG07383 ; PA3996 .
    GeneIDi 878931.
    KEGGi pae:PA3996.
    PATRICi 19842663. VBIPseAer58763_4188.

    Organism-specific databases

    PseudoCAPi PA3996.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi RKVDCDI.
    OrthoDBi EOG6038ZS.
    PhylomeDBi Q9HX25.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiLIPA_PSEAE
    AccessioniPrimary (citable) accession number: Q9HX25
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 26, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3