Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9HX25

- LIPA_PSEAE

UniProt

Q9HX25 - LIPA_PSEAE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Lipoyl synthase

Gene

lipA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi100 – 1001Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi104 – 1041Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:PA3996
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA3996.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Lipoyl synthasePRO_0000102342Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3996.

Structurei

3D structure databases

ProteinModelPortaliQ9HX25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
InParanoidiQ9HX25.
KOiK03644.
OMAiRKVDCDI.
OrthoDBiEOG6038ZS.
PhylomeDBiQ9HX25.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HX25-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTVVEKSGE AKPGKVEVGV KLRGAEKVAR IPVKIIPTEE LPKKPDWIRV
60 70 80 90 100
RIPVSPEVDR IKQLLRKHKL HSVCEEASCP NLGECFSGGT ATFMIMGDIC
110 120 130 140 150
TRRCPFCDVG HGRPKPLDVD EPTNLAIAIA DLRLKYVVIT SVDRDDLRDG
160 170 180 190 200
GAQHFADCLR EIRKLSPGIQ LETLVPDYRG RMDIALEITA NEPPDVFNHN
210 220 230 240 250
LETVPRLYRS SRPGSDFEWS LDLLQKFKQM VPHVPTKSGL MLGLGETDDE
260 270 280 290 300
VIEVMQRMRE HDIDMLTLGQ YLQPSRNHLP VQRFVHPDTF AWFAEEGEKM
310 320
GFKNVASGPL VRSSYHADQQ AHGNKIG
Length:327
Mass (Da):36,733
Last modified:March 1, 2001 - v1
Checksum:i0A0C8BDD44681753
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG07383.1.
PIRiH83145.
RefSeqiNP_252685.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG07383; AAG07383; PA3996.
GeneIDi878931.
KEGGipae:PA3996.
PATRICi19842663. VBIPseAer58763_4188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG07383.1 .
PIRi H83145.
RefSeqi NP_252685.1. NC_002516.2.

3D structure databases

ProteinModelPortali Q9HX25.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA3996.

Protocols and materials databases

DNASUi 878931.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG07383 ; AAG07383 ; PA3996 .
GeneIDi 878931.
KEGGi pae:PA3996.
PATRICi 19842663. VBIPseAer58763_4188.

Organism-specific databases

PseudoCAPi PA3996.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
InParanoidi Q9HX25.
KOi K03644.
OMAi RKVDCDI.
OrthoDBi EOG6038ZS.
PhylomeDBi Q9HX25.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiLIPA_PSEAE
AccessioniPrimary (citable) accession number: Q9HX25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3