Reviewed, UniProtKB/Swiss-Prot Q9HWP3 (SYY1_PSEAE)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase 1
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase 1
      Short name=TyrRS 1

Gene names

Name:	tyrS1
Ordered Locus Names:	PA4138

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Tyrosyl-tRNA synthetase 1 HAMAP MF_02006		PRO_0000234753
Regions
	Domain	348 – 411	64	S4 RNA-binding
	Motif	46 – 55	10	"HIGH" region HAMAP MF_02006
	Motif	234 – 238	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	41	1	Tyrosine By similarity
	Binding site	174	1	Tyrosine By similarity
	Binding site	178	1	Tyrosine By similarity
	Binding site	237	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HWP3-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: A3B49DAD41B7A980	FASTA	412	45,177
	10 20 30 40 50 60 MSVPTHQQDL IALLEERGFV HQCTDRDGLA AHLAAGPATA YLGFDATADS LHVGHLQGLM 70 80 90 100 110 120 LMRWLQKAGH RPLLLIGGAT TRIGDPSFRD SSRPILTEAQ IQANIDGIAR VFSRYVELHD 130 140 150 160 170 180 DSLVNNAEWL DGVGYLEFLD RVGRHFSINR LLTFDAIRQR LDREHSLSFL EFGYTLLQAY 190 200 210 220 230 240 DFVELSRRRG CTLQLGGADQ WANIINGVEL SRRQGGAQLF GLTMPLLATS DGRKMGKSAQ 250 260 270 280 290 300 GAVWLNAERL APFDFWQFWR NCDDRDVGRF LALFSELPMD EVRRLGALQG AELNEAKVVL 310 320 330 340 350 360 ANAATALAHG EHAARSAADA ARGVFADGTR DSGLPVMKLS RARLAQGLSL TDLLLEHAIQ 370 380 390 400 410 PSRSAVRRLA AGGGLRLDGT PVSDPDTPLA GEVDGLRLSL GKKQHLHLRL ED

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG07525.1.
PIR	A83129.
RefSeq	NP_252827.1.
3D structure databases
HSSP	HSSP built from PDB template 2TS1 based on UniProtKB P00952.
ModBase	Search...
Genome annotation databases
GeneID	880105.
GenomeReviews	Gene locus PA4138 in contig AE004091_GR.
KEGG	pae:PA4138.
NMPDR	fig|208964.1.peg.4137.
Organism-specific databases
PseudoCAP	PA4138.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HWP3.
OMA	TDPTGDS.
Enzyme and pathway databases
BioCyc	PAER208964:PA4138-MON.
BRENDA	6.1.1.1. 354.
Family and domain databases
HAMAP	MF_02006. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY1_PSEAE

Accession

Primary (citable) accession number: Q9HWP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)