Q9HWK6 (LYSC_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysyl endopeptidase
EC=3.4.21.50

Alternative name(s):
Protease IV
pvdS-regulated endoprotease

Gene names

Name:	prpL
Ordered Locus Names:	PA4175

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Lysine-specific endoprotease. Involved in corneal virulence.
Catalytic activity	Preferential cleavage: Lys-|-Xaa, including Lys-|-Pro.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 211	187			PRO_0000228678
	Chain	212 – 462	251	Lysyl endopeptidase		PRO_0000228679
Sites
	Active site	283	1	Charge relay system By similarity
	Active site	333	1	Charge relay system By similarity
	Active site	409	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	224 ↔ 435		By similarity
	Disulfide bond	230 ↔ 305		By similarity
	Disulfide bond	262 ↔ 284		By similarity
Experimental info
	Mutagenesis	283	1	H → A: Loss of activity. Ref.1
	Mutagenesis	327	1	H → A: No loss of activity. Ref.1
	Mutagenesis	333	1	D → A: Loss of activity. Ref.1
	Mutagenesis	408	1	S → A: Loss of activity. Ref.1
	Mutagenesis	409	1	S → A: Loss of activity. Ref.1
	Mutagenesis	411	1	S → A: No loss of activity. Ref.1
	Sequence conflict	23	1	L → S in AAL47683. Ref.1
	Sequence conflict	57	1	I → T in AAL47683. Ref.1
	Sequence conflict	102	1	T → A in AAL47683. Ref.1
	Sequence conflict	137	1	H → R Ref.1
	Sequence conflict	137	1	H → R Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9HWK6 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 8988A6AE53E9C816	FASTA	462	48,213
	10 20 30 40 50 60 MHKRTYLNAC LVLALAAGAS QALAAPGASE MAGDVAVLQA SPASTGHARF ANPNAAISAA 70 80 90 100 110 120 GIHFAAPPAR RVARAAPLAP KPGTPLQVGV GLKTATPEID LTTLEWIDTP DGRHTARFPI 130 140 150 160 170 180 SAAGAASLRA AIRLETHSGS LPDDVLLHFA GAGKEIFEAS GKDLSVNRPY WSPVIEGDTL 190 200 210 220 230 240 TVELVLPANL QPGDLRLSVP QVSYFADSLY KAGYRDGFGA SGSCEVDAVC ATQSGTRAYD 250 260 270 280 290 300 NATAAVAKMV FTSSADGGSY ICTGTLLNNG NSPKRQLFWS AAHCIEDQAT AATLQTIWFY 310 320 330 340 350 360 NTTQCYGDAS TINQSVTVLT GGANILHRDA KRDTLLLELK RTPPAGVFYQ GWSATPIANG 370 380 390 400 410 420 SLGHDIHHPR GDAKKYSQGN VSAVGVTYDG HTALTRVDWP SAVVEGGSSG SGLLTVAGDG 430 440 450 460 SYQLRGGLYG GPSYCGAPTS QRNDYFSDFS GVYSQISRYF AP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the active site residues of Pseudomonas aeruginosa protease IV. Importance of enzyme activity in autoprocessing and activation." Traidej M., Marquart M.E., Caballero A.R., Thibodeaux B.A., O'Callaghan R.J. J. Biol. Chem. 278:2549-2553(2003) [PubMed: 12419815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-283; HIS-327; ASP-333; SER-408; SER-409 AND SER-411. Strain: PA103-29.
[2]	"PrpL protease of Pseudomonas aeruginosa: an important virulence determinant in corneal infections." Parveen N., Parker D.S., Fan L., Leong J.M., Goguen J.D. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PA4481.
[3]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[4]	Lahnstein J. Submitted (APR-2000) to UniProtKB Cited for: PROTEIN SEQUENCE OF 212-226.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY062882 Genomic DNA. Translation: AAL47683.1. AY850373 Genomic DNA. Translation: AAW33983.1. AE004091 Genomic DNA. Translation: AAG07562.1.
PIR	B83123.
RefSeq	NP_252864.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9HWK6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	880208.
GenomeReviews	Gene locus PA4175 in contig AE004091_GR.
KEGG	pae:PA4175.
NMPDR	fig|208964.1.peg.4174.
PATRIC	19843036. VBIPseAer58763_4374.
Organism-specific databases
PseudoCAP	PA4175.
Phylogenomic databases
HOGENOM	HBG752136.
OMA	PIANGSL.
ProtClustDB	CLSK2297761.
Enzyme and pathway databases
BioCyc	PAER208964:PA4175-MONOMER.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LYSC_PSEAE

Accession

Primary (citable) accession number: Q9HWK6
Secondary accession number(s): P82468, Q5I6A6, Q8VPA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program