Q9HWK6 (LYSC_PSEAE) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 59.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Lysyl endopeptidase EC=3.4.21.50 Alternative name(s): Protease IV pvdS-regulated endoprotease | ||||
| Gene names |
| ||||
| Organism | Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) | ||||
| Taxonomic identifier | 208964 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas |
Protein attributes
| Sequence length | 462 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Lysine-specific endoprotease. Involved in corneal virulence. |
| Catalytic activity | Preferential cleavage: Lys-|-Xaa, including Lys-|-Pro. |
| Subcellular location | |
| Sequence similarities | Belongs to the peptidase S1 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Secreted |
| Domain | Signal |
| Molecular function | Hydrolase Protease Serine protease |
| PTM | Disulfide bond Zymogen |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | proteolysis Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 24 | 24 | Potential | ||||||||
| Propeptide | 25 – 211 | 187 | PRO_0000228678 | ||||||||
| Chain | 212 – 462 | 251 | Lysyl endopeptidase | PRO_0000228679 | |||||||
Sites | |||||||||||
| Active site | 283 | 1 | Charge relay system By similarity | ||||||||
| Active site | 333 | 1 | Charge relay system By similarity | ||||||||
| Active site | 409 | 1 | Charge relay system By similarity | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 224 ↔ 435 | By similarity | |||||||||
| Disulfide bond | 230 ↔ 305 | By similarity | |||||||||
| Disulfide bond | 262 ↔ 284 | By similarity | |||||||||
Experimental info | |||||||||||
| Mutagenesis | 283 | 1 | H → A: Loss of activity. Ref.1 | ||||||||
| Mutagenesis | 327 | 1 | H → A: No loss of activity. Ref.1 | ||||||||
| Mutagenesis | 333 | 1 | D → A: Loss of activity. Ref.1 | ||||||||
| Mutagenesis | 408 | 1 | S → A: Loss of activity. Ref.1 | ||||||||
| Mutagenesis | 409 | 1 | S → A: Loss of activity. Ref.1 | ||||||||
| Mutagenesis | 411 | 1 | S → A: No loss of activity. Ref.1 | ||||||||
| Sequence conflict | 23 | 1 | L → S in AAL47683. Ref.1 | ||||||||
| Sequence conflict | 57 | 1 | I → T in AAL47683. Ref.1 | ||||||||
| Sequence conflict | 102 | 1 | T → A in AAL47683. Ref.1 | ||||||||
| Sequence conflict | 137 | 1 | H → R Ref.1 | ||||||||
| Sequence conflict | 137 | 1 | H → R Ref.2 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of the active site residues of Pseudomonas aeruginosa protease IV. Importance of enzyme activity in autoprocessing and activation." Traidej M., Marquart M.E., Caballero A.R., Thibodeaux B.A., O'Callaghan R.J. J. Biol. Chem. 278:2549-2553(2003) [PubMed: 12419815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-283; HIS-327; ASP-333; SER-408; SER-409 AND SER-411. Strain: PA103-29. |
| [2] | "PrpL protease of Pseudomonas aeruginosa: an important virulence determinant in corneal infections." Parveen N., Parker D.S., Fan L., Leong J.M., Goguen J.D. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PA4481. |
| [3] | "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. Olson M.V.Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228. |
| [4] | Lahnstein J. Submitted (APR-2000) to UniProtKB Cited for: PROTEIN SEQUENCE OF 212-226. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY062882 Genomic DNA. Translation: AAL47683.1. AY850373 Genomic DNA. Translation: AAW33983.1. AE004091 Genomic DNA. Translation: AAG07562.1. |
| PIR | B83123. |
| RefSeq | NP_252864.1. NC_002516.2. |
3D structure databases | |
| ProteinModelPortal | Q9HWK6. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 880208. |
| GenomeReviews | Gene locus PA4175 in contig AE004091_GR. |
| KEGG | pae:PA4175. |
| NMPDR | fig|208964.1.peg.4174. |
| PATRIC | 19843036. VBIPseAer58763_4374. |
Organism-specific databases | |
| PseudoCAP | PA4175. |
Phylogenomic databases | |
| HOGENOM | HBG752136. |
| OMA | PIANGSL. |
| ProtClustDB | CLSK2297761. |
Enzyme and pathway databases | |
| BioCyc | PAER208964:PA4175-MONOMER. |
Family and domain databases | |
| InterPro | IPR009003. Pept_cys/ser_Trypsin-like. [Graphical view] |
| SUPFAM | SSF50494. Pept_Ser_Cys. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | LYSC_PSEAE | ||||||||
| Accession | Primary (citable) accession number: Q9HWK6 Secondary accession number(s): P82468, Q5I6A6, Q8VPA4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with