Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9HWI0 (DDLA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase A

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase A
D-alanylalanine synthetase A
Gene names
Name:ddlA
Ordered Locus Names:PA4201
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346D-alanine--D-alanine ligase A HAMAP-Rule MF_00047
PRO_0000177855

Regions

Domain138 – 332195ATP-grasp
Nucleotide binding164 – 21754ATP By similarity

Sites

Metal binding2861Magnesium or manganese 1 By similarity
Metal binding2991Magnesium or manganese 1 By similarity
Metal binding2991Magnesium or manganese 2 By similarity
Metal binding3011Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9HWI0 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: A66C5B0DB58F9A23

FASTA34636,534
        10         20         30         40         50         60 
MGQDKLKVAV LFGGSSEERD VSIASGAQVI QALRSAGHQV LAVDTASGLL GAEEERRLLA 

        70         80         90        100        110        120 
SKVKEVPPDS DSLAIIRSGK QSLLSAGELA GVDVFFLALH GGTGEDGTLQ ALLDAGGFAY 

       130        140        150        160        170        180 
TGSGHLASAM AMDKDVAKRL FLAAGVETAS WLMAPASEEE VREQLGFPLV VKPNSQGSTV 

       190        200        210        220        230        240 
GLSIVHSQAE LQPAIELAGR YGDEVMLERF VAGREVTVGV LDDQALPVGE ILLGGQEVFD 

       250        260        270        280        290        300 
YEHKYQAGAV REVFPADLPP AIAAEAQRLA LKVHRALKLS GYSRTDFRLD EQGRLWCLEV 

       310        320        330        340 
NTLPGMTATS LLPQAAAAAG IGFAELCERI CRLGIERCKG ARKARS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004091 Genomic DNA. Translation: AAG07588.1.
PIRE83121.
RefSeqNP_252890.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9HWI0.
SMRQ9HWI0. Positions 7-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA4201.

Protocols and materials databases

DNASU879033.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG07588; AAG07588; PA4201.
GeneID879033.
KEGGpae:PA4201.
PATRIC19843090. VBIPseAer58763_4401.

Organism-specific databases

PseudoCAPPA4201.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADYTARYT.
OrthoDBEOG6ND0KB.
PhylomeDBQ9HWI0.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDLA_PSEAE
AccessionPrimary (citable) accession number: Q9HWI0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways