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Protein

Bacterioferritin

Gene

bfr

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Iron-storage protein, whose ferroxidase center binds Fe2+ ions, oxidizes them by dioxygen to Fe3+, and participates in the subsequent Fe3+ oxide mineral core formation within the central cavity of the protein complex.By similarity

Catalytic activityi

4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bCuratedNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.Curated
  • Fe cationBy similarityNote: Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi18 – 181Iron 1PROSITE-ProRule annotation
Metal bindingi48 – 481Iron (heme axial ligand); shared with dimeric partnerPROSITE-ProRule annotation
Metal bindingi51 – 511Iron 1PROSITE-ProRule annotation
Metal bindingi51 – 511Iron 2PROSITE-ProRule annotation
Metal bindingi54 – 541Iron 1PROSITE-ProRule annotation
Metal bindingi93 – 931Iron 2PROSITE-ProRule annotation
Metal bindingi127 – 1271Iron 1PROSITE-ProRule annotation
Metal bindingi127 – 1271Iron 2PROSITE-ProRule annotation
Metal bindingi130 – 1301Iron 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • intracellular sequestering of iron ion Source: PseudoCAP
  • iron ion transport Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Iron storage

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bacterioferritin (EC:1.16.3.1)
Short name:
BFR
Gene namesi
Name:bfr
Synonyms:bfrA
Ordered Locus Names:PA4235
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4235.

Subcellular locationi

GO - Cellular componenti

  • cell Source: GOC
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 154154BacterioferritinPRO_0000287751Add
BLAST

Interactioni

Subunit structurei

Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited.By similarity

Protein-protein interaction databases

STRINGi208964.PA4235.

Structurei

Secondary structure

1
154
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 3430Combined sources
Helixi38 – 6427Combined sources
Helixi82 – 10928Combined sources
Helixi113 – 12816Combined sources
Helixi130 – 14415Combined sources
Helixi146 – 1527Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3R2HX-ray1.70A1-154[»]
3R2KX-ray1.55A1-154[»]
3R2LX-ray1.85A1-154[»]
3R2MX-ray1.80A1-154[»]
3R2OX-ray1.95A1-154[»]
3R2RX-ray1.65A1-154[»]
3R2SX-ray2.10A1-154[»]
ProteinModelPortaliQ9HWF9.
SMRiQ9HWF9. Positions 1-154.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HWF9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 145145Ferritin-like diironPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the bacterioferritin family.Curated
Contains 1 ferritin-like diiron domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2193.
HOGENOMiHOG000262383.
InParanoidiQ9HWF9.
KOiK03594.
OMAiAIDQYFI.
OrthoDBiEOG6WDSKP.
PhylomeDBiQ9HWF9.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR002024. Bacterioferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
PIRSFiPIRSF002560. Bacterioferritin. 1 hit.
PRINTSiPR00601. BACFERRITIN.
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR00754. bfr. 1 hit.
PROSITEiPS00549. BACTERIOFERRITIN. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HWF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGHPEVIDY LNTLLTGELA ARDQYFIHSR MYEDWGFSKL YERLNHEMEE
60 70 80 90 100
ETQHADALLR RILLLEGTPR MRPDDIHPGT TVPEMLEADL KLERHVRAAL
110 120 130 140 150
AKGIALCEQH KDFVSRDILK AQLADTEEDH AYWLEQQLGL IARMGLENYL

QSQI
Length:154
Mass (Da):17,940
Last modified:March 1, 2001 - v1
Checksum:iDFD36D0DB9518343
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07623.1.
PIRiA83113.
RefSeqiNP_252925.1. NC_002516.2.
WP_003093668.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG07623; AAG07623; PA4235.
GeneIDi881830.
KEGGipae:PA4235.
PATRICi19843161. VBIPseAer58763_4436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07623.1.
PIRiA83113.
RefSeqiNP_252925.1. NC_002516.2.
WP_003093668.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3R2HX-ray1.70A1-154[»]
3R2KX-ray1.55A1-154[»]
3R2LX-ray1.85A1-154[»]
3R2MX-ray1.80A1-154[»]
3R2OX-ray1.95A1-154[»]
3R2RX-ray1.65A1-154[»]
3R2SX-ray2.10A1-154[»]
ProteinModelPortaliQ9HWF9.
SMRiQ9HWF9. Positions 1-154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4235.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07623; AAG07623; PA4235.
GeneIDi881830.
KEGGipae:PA4235.
PATRICi19843161. VBIPseAer58763_4436.

Organism-specific databases

PseudoCAPiPA4235.

Phylogenomic databases

eggNOGiCOG2193.
HOGENOMiHOG000262383.
InParanoidiQ9HWF9.
KOiK03594.
OMAiAIDQYFI.
OrthoDBiEOG6WDSKP.
PhylomeDBiQ9HWF9.

Miscellaneous databases

EvolutionaryTraceiQ9HWF9.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR002024. Bacterioferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
PIRSFiPIRSF002560. Bacterioferritin. 1 hit.
PRINTSiPR00601. BACFERRITIN.
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR00754. bfr. 1 hit.
PROSITEiPS00549. BACTERIOFERRITIN. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiBFR_PSEAE
AccessioniPrimary (citable) accession number: Q9HWF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.