Bacterioferritin - Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Bacterioferritin
Short name=BFR
EC=1.16.3.1

Gene names

Name:	bfr
Synonyms:	bfrA
Ordered Locus Names:	PA4235

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Iron-storage protein, whose ferroxidase center binds Fe²⁺ ions, oxidizes them by dioxygen to Fe³⁺, and participates in the subsequent Fe³⁺ oxide mineral core formation within the central cavity of the protein complex By similarity.
Catalytic activity	4 Fe²⁺ + 4 H⁺ + O₂ = 4 Fe³⁺ + 2 H₂O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer Potential. Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center By similarity.
Subunit structure	Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited By similarity.
Sequence similarities	Belongs to the bacterioferritin family. Contains 1 ferritin-like diiron domain.

Ontologies

Keywords
Biological process	Iron storage
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW iron ion transport Inferred from electronic annotation. Source: InterPro
Molecular_function	ferric iron binding Inferred from electronic annotation. Source: InterPro ferroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 154

154

Bacterioferritin

PRO_0000287751

Regions

Domain

1 – 145

145

Ferritin-like diiron

Sites

Metal binding

18

1

Iron 1 By similarity

Metal binding

48

1

Iron (heme axial ligand); shared with dimeric partner Potential

Metal binding

51

1

Iron 1 By similarity

Metal binding

51

1

Iron 2 By similarity

Metal binding

54

1

Iron 1 By similarity

Metal binding

93

1

Iron 2 By similarity

Metal binding

127

1

Iron 1 By similarity

Metal binding

127

1

Iron 2 By similarity

Metal binding

130

1

Iron 2 By similarity

Secondary structure

1

.

154

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9HWF9 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: DFD36D0DB9518343
Show »

FASTA

154

17,940

        10         20         30         40         50         60 
MQGHPEVIDY LNTLLTGELA ARDQYFIHSR MYEDWGFSKL YERLNHEMEE ETQHADALLR 

        70         80         90        100        110        120 
RILLLEGTPR MRPDDIHPGT TVPEMLEADL KLERHVRAAL AKGIALCEQH KDFVSRDILK 

       130        140        150 
AQLADTEEDH AYWLEQQLGL IARMGLENYL QSQI

« Hide

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R.

Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE004091 Genomic DNA. Translation: AAG07623.1.

PIR

A83113.

RefSeq

NP_252925.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3R2H	X-ray	1.70	A	1-154	[»]
3R2K	X-ray	1.55	A	1-154	[»]
3R2L	X-ray	1.85	A	1-154	[»]
3R2M	X-ray	1.80	A	1-154	[»]
3R2O	X-ray	1.95	A	1-154	[»]
3R2R	X-ray	1.65	A	1-154	[»]
3R2S	X-ray	2.10	A	1-154	[»]

ProteinModelPortal

Q9HWF9.

SMR

Q9HWF9. Positions 1-154.

ModBase

Search...

Protein-protein interaction databases

STRING

208964.PA4235.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

881830.

KEGG

pae:PA4235.

PATRIC

19843161. VBIPseAer58763_4436.

Organism-specific databases

PseudoCAP

PA4235.

Phylogenomic databases

eggNOG

COG2193.

HOGENOM

HOG000262383.

KO

K03594.

OMA

MDQYFVH.

ProtClustDB

CLSK868542.

Family and domain databases

Gene3D

1.20.1260.10. 1 hit.

InterPro

IPR002024. Bacterioferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]

Pfam

PF00210. Ferritin. 1 hit.
[Graphical view]

PIRSF

PIRSF002560. Bacterioferritin. 1 hit.

PRINTS

PR00601. BACFERRITIN.

SUPFAM

SSF47240. Ferritin/RR_like. 1 hit.

TIGRFAMs

TIGR00754. bfr. 1 hit.

PROSITE

PS00549. BACTERIOFERRITIN. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9HWF9.

Entry information

Entry name

BFR_PSEAE

Accession

Primary (citable) accession number: Q9HWF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families