Reviewed, UniProtKB/Swiss-Prot Q9HW26 (PNCB2_PSEAE)

Names and origin

Protein names

Recommended name:
    Nicotinate phosphoribosyltransferase 2
      Short name=NAPRTase 2
    EC=2.4.2.11

Gene names

Name:	pncB2
Ordered Locus Names:	PA4376

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00570
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1. HAMAP MF_00570
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAPRTase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP nicotinate nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nicotinate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 398

398

Nicotinate phosphoribosyltransferase 2 HAMAP MF_00570

PRO_0000205838

Secondary structure

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398

Helix Strand Turn

Details...

Beta strand

16 – 18

3

Helix

19 – 31

13

Beta strand

36 – 43

8

Helix

50 – 52

3

Helix

53 – 65

13

Helix

70 – 77

8

Helix

84 – 92

9

Helix

97 – 99

3

Beta strand

100 – 105

6

Beta strand

108 – 116

9

Helix

117 – 120

4

Helix

121 – 123

3

Helix

124 – 139

16

Helix

145 – 160

16

Helix

165 – 168

4

Beta strand

172 – 175

4

Helix

184 – 197

14

Beta strand

199 – 207

9

Helix

208 – 213

6

Helix

224 – 233

10

Helix

237 – 239

3

Helix

240 – 252

13

Beta strand

258 – 261

4

Helix

266 – 272

7

Helix

275 – 280

6

Beta strand

283 – 286

4

Helix

291 – 305

15

Helix

309 – 311

3

Beta strand

312 – 316

5

Helix

322 – 332

11

Turn

333 – 335

3

Beta strand

336 – 342

7

Helix

344 – 347

4

Beta strand

358 – 365

8

Helix

386 – 395

10

Sequences

	Sequence		Length	Mass (Da)
	Q9HW26-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 526899B1B705C37F	FASTA	398	45,691
	10 20 30 40 50 60 MAESAFSERI VQNLLDTDFY KLTMMQAVLH NYPNAEVEWE FRCRNQEDLR LYLPAIREQL 70 80 90 100 110 120 EYLAGLAISD EQLAFLERIP FLAPDFIRFL GLFRFNPRYV QTGIENDEFF LRLKGPWLHV 130 140 150 160 170 180 ILFEVPLLAM ISEVRNRARY PAATVEQARE RLQEKFDWLR REASAEELAG FKMADFGTRR 190 200 210 220 230 240 RFSYRVHEAV VSGLKEDFPG CFVGTSNVHL ARKLDLKPLG TMAHEWLMAH QQLGPRLIDS 250 260 270 280 290 300 QSAALDCWVR EYRGLLGIAL TDCITTDAFL RDFDLYFAKL FDGLRHDSGD PLLWAEKTIA 310 320 330 340 350 360 HYLKLGIDPL TKTLVFSDGL DLPRALKIYR ALQGRINVSF GIGTHFTCDL PGVEPMNIVV 370 380 390 KMSACNGHPV AKISDTPGKA QCRDPDFIHY LKHVFQVA

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE004091 Genomic DNA. Translation: AAG07764.1.

PIR

F83099.

RefSeq

NP_253066.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YIR	X-ray	2.10	A/B/C/D	2-398	[»]

ModBase

Search...

Genome annotation databases

GeneID

881378.

GenomeReviews

Gene locus PA4376 in contig AE004091_GR.

KEGG

pae:PA4376.

Organism-specific databases

PseudoCAP

PA4376.

CMR

Search...

Phylogenomic databases

HOGENOM

Q9HW26.

OMA

IREQIEY.

Enzyme and pathway databases

BioCyc

PAER208964:PA4376-MON.

BRENDA

2.4.2.11. 354.

Family and domain databases

HAMAP

MF_00570.
[Tree]

InterPro

IPR006406. Nic_PRibTrfase.
IPR015977. Nic_PRibTrfase-like.
IPR007229. Nic_PRibTrfase-rel.
[Graphical view]

PANTHER

PTHR11098:SF1. NAPRTase. 1 hit.

Pfam

PF04095. NAPRTase. 1 hit.
[Graphical view]

PIRSF

PIRSF000484. NAPRT. 1 hit.

TIGRFAMs

TIGR01514. NAPRTase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PNCB2_PSEAE

Accession

Primary (citable) accession number: Q9HW26

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)