Q9HW09 (PANE_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 2-dehydropantoate 2-reductase
EC=1.1.1.169

Alternative name(s):
Ketopantoate reductase
Short name=KPA reductase
Short name=KPR

Gene names

Name:	panE
Ordered Locus Names:	PA4397

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Probable 2-dehydropantoate 2-reductase		PRO_0000157308
Regions
	Nucleotide binding	7 – 12	6	NADP By similarity
Sites
	Active site	182	1	Proton donor By similarity
	Binding site	31	1	NADP; via amide nitrogen By similarity
	Binding site	103	1	NADP; via amide nitrogen By similarity
	Binding site	103	1	Substrate By similarity
	Binding site	129	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	186	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity
	Binding site	200	1	Substrate By similarity
	Binding site	247	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity
	Binding site	262	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HW09 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 664A30DF3028E1D8	FASTA	303	33,062
	10 20 30 40 50 60 MTWHILGAGS LGSLWAARLG RAGLPVRLIL RDRQRLRRYQ QAGGLSLVED GQASLYPIAA 70 80 90 100 110 120 ETPDGGQPIQ RLLLACKAYD AEEAASSVAH RLAGNAELLL LQNGLGSQQA VAARLPRSRC 130 140 150 160 170 180 LFASSTEGAF RDGDFRVVFA GRGHTWLGDP RDTNAPAWLT QLSQAGIPHS WSDDILERLW 190 200 210 220 230 240 RKLALNCAIN PLTVLHDCRN GGLRQHPEEI AALCDELGQL LHASGYDAAA RSLLEDVRAV 250 260 270 280 290 300 IDATAANYSS MHQDVTRGRR TEIGYLLGYA CQHGQRLGLP LPRLGTLLAR LQAHLRQRGL PDR

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG07785.1.
PIR	E83096.
RefSeq	NP_253087.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9HW09.
SMR	Q9HW09. Positions 1-293.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA4397.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	881354.
KEGG	pae:PA4397.
PATRIC	19843517. VBIPseAer58763_4605.
Organism-specific databases
PseudoCAP	PA4397.
Phylogenomic databases
eggNOG	COG1893.
HOGENOM	HOG000050223.
KO	K00077.
OMA	CIRDGRC.
ProtClustDB	PRK05708.
Enzyme and pathway databases
UniPathway	UPA00028; UER00004.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANE_PSEAE

Accession

Primary (citable) accession number: Q9HW09

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program