Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable 2-dehydropantoate 2-reductase

Gene

panE

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.

Catalytic activityi

(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase 2 (panB2), 3-methyl-2-oxobutanoate hydroxymethyltransferase 1 (panB1)
  2. 2-dehydropantoate 2-reductase (PA1752), Probable 2-dehydropantoate 2-reductase (panE)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31NADP; via amide nitrogenBy similarity1
Binding sitei103NADP; via amide nitrogenBy similarity1
Binding sitei103SubstrateBy similarity1
Binding sitei129NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei182Proton donorBy similarity1
Binding sitei186SubstrateBy similarity1
Binding sitei190SubstrateBy similarity1
Binding sitei200SubstrateBy similarity1
Binding sitei247SubstrateBy similarity1
Binding sitei250SubstrateBy similarity1
Binding sitei262NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 12NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pantothenate biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00028; UER00004.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 2-dehydropantoate 2-reductase (EC:1.1.1.169)
Alternative name(s):
Ketopantoate reductase
Short name:
KPA reductase
Short name:
KPR
Gene namesi
Name:panE
Ordered Locus Names:PA4397
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4397.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001573081 – 303Probable 2-dehydropantoate 2-reductaseAdd BLAST303

Proteomic databases

PaxDbiQ9HW09.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4397.

Structurei

3D structure databases

ProteinModelPortaliQ9HW09.
SMRiQ9HW09.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ketopantoate reductase family.Curated

Phylogenomic databases

eggNOGiENOG4108JZF. Bacteria.
COG1893. LUCA.
HOGENOMiHOG000050223.
InParanoidiQ9HW09.
KOiK00077.
OMAiRIPQPYC.
PhylomeDBiQ9HW09.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR003710. ApbA.
IPR013752. KPA_reductase.
IPR013332. KPR_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02558. ApbA. 1 hit.
PF08546. ApbA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00745. apbA_panE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HW09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTWHILGAGS LGSLWAARLG RAGLPVRLIL RDRQRLRRYQ QAGGLSLVED
60 70 80 90 100
GQASLYPIAA ETPDGGQPIQ RLLLACKAYD AEEAASSVAH RLAGNAELLL
110 120 130 140 150
LQNGLGSQQA VAARLPRSRC LFASSTEGAF RDGDFRVVFA GRGHTWLGDP
160 170 180 190 200
RDTNAPAWLT QLSQAGIPHS WSDDILERLW RKLALNCAIN PLTVLHDCRN
210 220 230 240 250
GGLRQHPEEI AALCDELGQL LHASGYDAAA RSLLEDVRAV IDATAANYSS
260 270 280 290 300
MHQDVTRGRR TEIGYLLGYA CQHGQRLGLP LPRLGTLLAR LQAHLRQRGL

PDR
Length:303
Mass (Da):33,062
Last modified:March 1, 2001 - v1
Checksum:i664A30DF3028E1D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07785.1.
PIRiE83096.
RefSeqiNP_253087.1. NC_002516.2.
WP_003106518.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG07785; AAG07785; PA4397.
GeneIDi881354.
KEGGipae:PA4397.
PATRICi19843517. VBIPseAer58763_4605.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07785.1.
PIRiE83096.
RefSeqiNP_253087.1. NC_002516.2.
WP_003106518.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ9HW09.
SMRiQ9HW09.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4397.

Proteomic databases

PaxDbiQ9HW09.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07785; AAG07785; PA4397.
GeneIDi881354.
KEGGipae:PA4397.
PATRICi19843517. VBIPseAer58763_4605.

Organism-specific databases

PseudoCAPiPA4397.

Phylogenomic databases

eggNOGiENOG4108JZF. Bacteria.
COG1893. LUCA.
HOGENOMiHOG000050223.
InParanoidiQ9HW09.
KOiK00077.
OMAiRIPQPYC.
PhylomeDBiQ9HW09.

Enzyme and pathway databases

UniPathwayiUPA00028; UER00004.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR003710. ApbA.
IPR013752. KPA_reductase.
IPR013332. KPR_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02558. ApbA. 1 hit.
PF08546. ApbA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00745. apbA_panE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPANE_PSEAE
AccessioniPrimary (citable) accession number: Q9HW09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2002
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.