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Q9HVZ9 (MURD_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9
Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Gene names
Name:murD
Ordered Locus Names:PA4414
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA). HAMAP MF_00639

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639

Subcellular location

Cytoplasm By similarity HAMAP MF_00639.

Sequence similarities

Belongs to the MurCDEF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 448448UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639
PRO_0000109062

Regions

Nucleotide binding116 – 1227ATP Potential

Experimental info

Sequence conflict571V → A in AAG45237. Ref.1
Sequence conflict2601D → G in AAG45237. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9HVZ9 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 0CFF8F1A7676B266

FASTA44848,080
        10         20         30         40         50         60 
MSLIASDHFR IVVGLGKSGM SLVRYLARRG LPFAVVDTRE NPPELATLRA QYPQVEVRCG 

        70         80         90        100        110        120 
ELDAEFLCSA RELYVSPGLS LRTPALVQAA AKGVRISGDI DLFAREAKAP IVAITGSNAK 

       130        140        150        160        170        180 
STVTTLVGEM AVAADKRVAV GGNLGTPALD LLADDIELYV LELSSFQLET CDRLNAEVAT 

       190        200        210        220        230        240 
VLNVSEDHMD RYDGMADYHL AKHRIFRGAR QVVVNRADAL TRPLIADTVP CWSFGLNKPD 

       250        260        270        280        290        300 
FKAFGLIEED GQKWLAFQFD KLLPVGELKI RGAHNYSNAL AALALGHAVG LPFDAMLGAL 

       310        320        330        340        350        360 
KAFSGLAHRC QWVRERQGVS YYDDSKATNV GAALAAIEGL GADIDGKLVL LAGGDGKGAD 

       370        380        390        400        410        420 
FHDLREPVAR FCRAVVLLGR DAGLIAQALG NAVPLVRVAT LDEAVRQAAE LAREGDAVLL 

       430        440 
SPACASLDMF KNFEERGRLF AKAVEELA

« Hide

References

« Hide 'large scale' references
[1]"The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa: cloning, production, and purification of active enzymes."
Azzolina B.A., Yuan X., Anderson M.S., El-Sherbeini M.
Protein Expr. Purif. 21:393-400(2001) [PubMed: 11281713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed: 10984043] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY008276 Genomic DNA. Translation: AAG45237.1.
AE004091 Genomic DNA. Translation: AAG07802.1.
PIRG83094.
RefSeqNP_253104.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9HVZ9.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID881268.
GenomeReviewsGene locus PA4414 in contig AE004091_GR.
KEGGpae:PA4414.
PATRIC19843553. VBIPseAer58763_4623.

Organism-specific databases

PseudoCAPPA4414.

Phylogenomic databases

HOGENOMHBG750024.
OMAACASWDM.
ProtClustDBPRK03803.

Enzyme and pathway databases

BioCycPAER208964:PA4414-MONOMER.

Family and domain databases

HAMAPMF_00639. MurD.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK01925.
PANTHERPTHR23135:SF2. PTHR23135:SF2. 1 hit.
PfamPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01087. MurD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURD_PSEAE
AccessionPrimary (citable) accession number: Q9HVZ9
Secondary accession number(s): Q9EY46
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents