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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei143L-tryptophanUniRule annotation1
Binding sitei197ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 12ATPUniRule annotation3
Nucleotide bindingi18 – 19ATPUniRule annotation2
Nucleotide bindingi155 – 157ATPUniRule annotation3
Nucleotide bindingi204 – 208ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-4527-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:PA4439
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4439

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366621 – 448Tryptophan--tRNA ligaseAdd BLAST448

Proteomic databases

PaxDbiQ9HVX6
PRIDEiQ9HVX6

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA4439

Structurei

3D structure databases

ProteinModelPortaliQ9HVX6
SMRiQ9HVX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 19"HIGH" regionUniRule annotation9
Motifi204 – 208"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059939
InParanoidiQ9HVX6
KOiK01867
OMAiQRMTQFK
PhylomeDBiQ9HVX6

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
IPR036913 YegP-like_sf
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
SUPFAMiSSF160113 SSF160113, 1 hit
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HVX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTRILTGIT PTGTPHLGNY AGAIRPAILA SRRSDVDSFY FLADYHALIK
60 70 80 90 100
CDDPARIQRS RLEIAATWLA GGLDVERATF YRQSDIPEIP ELTWLLTCVS
110 120 130 140 150
AKGLLNRAHA YKAAVDRNVE AGEDPDAGVT MGLYSYPVLM AADILMFNAH
160 170 180 190 200
KIPVGRDQVQ HVEMARDIGQ RFNHLFGNGR EFFVLPEAVI EENVATLPGL
210 220 230 240 250
DGRKMSKSYD NTIPLFSPSR QLKDAIARIV TDSRAPGEPK DPDSSHLFLL
260 270 280 290 300
YSAFASAEQV AAFRQELLEG LAWGEAKQRL FQLLDNELGE ARERYQALIA
310 320 330 340 350
KPDDIEDILL AGAAKARRIA TPFIAELREA VGLRSLREPL KSAESGKKKA
360 370 380 390 400
AKAARLVSFR DDDGSFRFRL LDAAGEQLLL SRAFADGKAA GAVSKRLLAG
410 420 430 440
ETADLRAEGN AFGLWLDGEA VAQSPAFADA AARDAAIERT REALAPQE
Length:448
Mass (Da):48,962
Last modified:March 1, 2001 - v1
Checksum:iFA82ECA10A049A84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG07827.1
PIRiA83091
RefSeqiNP_253129.1, NC_002516.2
WP_003112748.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG07827; AAG07827; PA4439
GeneIDi880958
KEGGipae:PA4439
PATRICifig|208964.12.peg.4648

Similar proteinsi

Entry informationi

Entry nameiSYW_PSEAE
AccessioniPrimary (citable) accession number: Q9HVX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health