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Protein

Lipoprotein signal peptidase

Gene

lspA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein specifically catalyzes the removal of signal peptides from prolipoproteins.UniRule annotation

Catalytic activityi

Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei115 – 1151UniRule annotation
Active sitei143 – 1431UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein signal peptidaseUniRule annotation (EC:3.4.23.36UniRule annotation)
Alternative name(s):
Prolipoprotein signal peptidaseUniRule annotation
Signal peptidase IIUniRule annotation
Short name:
SPase IIUniRule annotation
Gene namesi
Name:lspAUniRule annotation
Synonyms:ls
Ordered Locus Names:PA4559
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4559.

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 2920HelicalUniRule annotationAdd
BLAST
Transmembranei41 – 6323HelicalUniRule annotationAdd
BLAST
Transmembranei67 – 8923HelicalUniRule annotationAdd
BLAST
Transmembranei96 – 11823HelicalUniRule annotationAdd
BLAST
Transmembranei133 – 15523HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 169169Lipoprotein signal peptidasePRO_0000178802Add
BLAST

Proteomic databases

PaxDbiQ9HVM5.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4559.

Structurei

Secondary structure

1
169
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni4 – 63Combined sources
Helixi10 – 123Combined sources
Helixi13 – 3321Combined sources
Beta strandi40 – 434Combined sources
Turni44 – 463Combined sources
Beta strandi47 – 548Combined sources
Helixi60 – 623Combined sources
Helixi69 – 8921Combined sources
Helixi96 – 11823Combined sources
Beta strandi119 – 13012Combined sources
Turni131 – 1333Combined sources
Helixi141 – 15414Combined sources
Helixi155 – 1573Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5DIRX-ray2.80A/B/C/D1-169[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A8 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105M02. Bacteria.
COG0597. LUCA.
HOGENOMiHOG000096993.
InParanoidiQ9HVM5.
KOiK03101.
OMAiDFASKQW.
PhylomeDBiQ9HVM5.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HVM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDVDRFGRL PWLWITVLVF VLDQVSKAFF QAELSMYQQI VVIPDLFSWT
60 70 80 90 100
LAYNTGAAFS FLADSSGWQR WLFALIAIVV SASLVVWLKR LKKGETWLAI
110 120 130 140 150
ALALVLGGAL GNLYDRMVLG HVVDFILVHW QNRWYFPAFN LADSAITVGA
160
VMLALDMFRS KKSGEAAHG
Length:169
Mass (Da):18,997
Last modified:March 1, 2001 - v1
Checksum:i1AE91A4B6B60006F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07947.1.
PIRiA83077.
RefSeqiNP_253249.1. NC_002516.2.
WP_003112823.1. NZ_ASJY01000736.1.

Genome annotation databases

EnsemblBacteriaiAAG07947; AAG07947; PA4559.
GeneIDi877620.
KEGGipae:PA4559.
PATRICi19843857. VBIPseAer58763_4771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07947.1.
PIRiA83077.
RefSeqiNP_253249.1. NC_002516.2.
WP_003112823.1. NZ_ASJY01000736.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5DIRX-ray2.80A/B/C/D1-169[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4559.

Proteomic databases

PaxDbiQ9HVM5.

Protocols and materials databases

DNASUi877620.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07947; AAG07947; PA4559.
GeneIDi877620.
KEGGipae:PA4559.
PATRICi19843857. VBIPseAer58763_4771.

Organism-specific databases

PseudoCAPiPA4559.

Phylogenomic databases

eggNOGiENOG4105M02. Bacteria.
COG0597. LUCA.
HOGENOMiHOG000096993.
InParanoidiQ9HVM5.
KOiK03101.
OMAiDFASKQW.
PhylomeDBiQ9HVM5.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLSPA_PSEAE
AccessioniPrimary (citable) accession number: Q9HVM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.