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Protein

Lipoprotein signal peptidase

Gene

lspA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein specifically catalyzes the removal of signal peptides from prolipoproteins.UniRule annotation

Catalytic activityi

Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115UniRule annotation1
Active sitei143UniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein signal peptidaseUniRule annotation (EC:3.4.23.36UniRule annotation)
Alternative name(s):
Prolipoprotein signal peptidaseUniRule annotation
Signal peptidase IIUniRule annotation
Short name:
SPase IIUniRule annotation
Gene namesi
Name:lspAUniRule annotation
Synonyms:ls
Ordered Locus Names:PA4559
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4559.

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei10 – 29HelicalUniRule annotationAdd BLAST20
Transmembranei41 – 63HelicalUniRule annotationAdd BLAST23
Transmembranei67 – 89HelicalUniRule annotationAdd BLAST23
Transmembranei96 – 118HelicalUniRule annotationAdd BLAST23
Transmembranei133 – 155HelicalUniRule annotationAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001788021 – 169Lipoprotein signal peptidaseAdd BLAST169

Proteomic databases

PaxDbiQ9HVM5.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4559.

Structurei

Secondary structure

1169
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni4 – 6Combined sources3
Helixi10 – 12Combined sources3
Helixi13 – 33Combined sources21
Beta strandi40 – 43Combined sources4
Turni44 – 46Combined sources3
Beta strandi47 – 54Combined sources8
Helixi60 – 62Combined sources3
Helixi69 – 89Combined sources21
Helixi96 – 118Combined sources23
Beta strandi119 – 130Combined sources12
Turni131 – 133Combined sources3
Helixi141 – 154Combined sources14
Helixi155 – 157Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DIRX-ray2.80A/B/C/D1-169[»]
ProteinModelPortaliQ9HVM5.
SMRiQ9HVM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A8 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105M02. Bacteria.
COG0597. LUCA.
HOGENOMiHOG000096993.
InParanoidiQ9HVM5.
KOiK03101.
OMAiDFASKQW.
PhylomeDBiQ9HVM5.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HVM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDVDRFGRL PWLWITVLVF VLDQVSKAFF QAELSMYQQI VVIPDLFSWT
60 70 80 90 100
LAYNTGAAFS FLADSSGWQR WLFALIAIVV SASLVVWLKR LKKGETWLAI
110 120 130 140 150
ALALVLGGAL GNLYDRMVLG HVVDFILVHW QNRWYFPAFN LADSAITVGA
160
VMLALDMFRS KKSGEAAHG
Length:169
Mass (Da):18,997
Last modified:March 1, 2001 - v1
Checksum:i1AE91A4B6B60006F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07947.1.
PIRiA83077.
RefSeqiNP_253249.1. NC_002516.2.
WP_003112823.1. NZ_ASJY01000736.1.

Genome annotation databases

EnsemblBacteriaiAAG07947; AAG07947; PA4559.
GeneIDi877620.
KEGGipae:PA4559.
PATRICi19843857. VBIPseAer58763_4771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07947.1.
PIRiA83077.
RefSeqiNP_253249.1. NC_002516.2.
WP_003112823.1. NZ_ASJY01000736.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DIRX-ray2.80A/B/C/D1-169[»]
ProteinModelPortaliQ9HVM5.
SMRiQ9HVM5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4559.

Proteomic databases

PaxDbiQ9HVM5.

Protocols and materials databases

DNASUi877620.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07947; AAG07947; PA4559.
GeneIDi877620.
KEGGipae:PA4559.
PATRICi19843857. VBIPseAer58763_4771.

Organism-specific databases

PseudoCAPiPA4559.

Phylogenomic databases

eggNOGiENOG4105M02. Bacteria.
COG0597. LUCA.
HOGENOMiHOG000096993.
InParanoidiQ9HVM5.
KOiK03101.
OMAiDFASKQW.
PhylomeDBiQ9HVM5.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLSPA_PSEAE
AccessioniPrimary (citable) accession number: Q9HVM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.