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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).By similarity

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021ATPBy similarity
Active sitei308 – 3081Tele-AMP-histidine intermediateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi283 – 2875ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Gene namesi
Name:rtcA
Ordered Locus Names:PA4585
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4585.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341RNA 3'-terminal phosphate cyclasePRO_0000156419Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4585.

Structurei

3D structure databases

ProteinModelPortaliQ9HVJ9.
SMRiQ9HVJ9. Positions 3-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
InParanoidiQ9HVJ9.
KOiK01974.
OMAiHYLALVW.
OrthoDBiEOG6RNQDX.
PhylomeDBiQ9HVJ9.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HVJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKDLIELDG SEGGGQILRS ALSLSMTSGQ PLRIRNIRGR RSRPGLLRQH
60 70 80 90 100
LTAVRAAAEI CAAEVEGAEL GSRELAFRPG AIRAGDYAFA IGSAGSCSLV
110 120 130 140 150
LQTLLPALLA ANGESRVRIS GGTHNPLAPP ADFLRDSWLP LLQRMGAEVD
160 170 180 190 200
LELLRHGFVP AGGGELLARV RPARWRPLQL EHPGAALRRQ ARALLAGIPG
210 220 230 240 250
HVGERELERV RQRLGWSDEE RQLEFLAEDQ GPGNALLLRI DCEHICATFC
260 270 280 290 300
AFGQAGVSAE RVAEQVATQA IGWMESGCAA DEHLADQLLL PMALAGAGSF
310 320 330 340
TTPRLSAHLQ SNRRVIERFL PVRIGDQALD GGGHRIVITS A
Length:341
Mass (Da):36,637
Last modified:March 1, 2001 - v1
Checksum:iE7277E130981D24F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07973.1.
PIRiE83072.
RefSeqiNP_253275.1. NC_002516.2.
WP_003112809.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG07973; AAG07973; PA4585.
GeneIDi881010.
KEGGipae:PA4585.
PATRICi19843915. VBIPseAer58763_4799.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG07973.1.
PIRiE83072.
RefSeqiNP_253275.1. NC_002516.2.
WP_003112809.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ9HVJ9.
SMRiQ9HVJ9. Positions 3-331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4585.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07973; AAG07973; PA4585.
GeneIDi881010.
KEGGipae:PA4585.
PATRICi19843915. VBIPseAer58763_4799.

Organism-specific databases

PseudoCAPiPA4585.

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
InParanoidiQ9HVJ9.
KOiK01974.
OMAiHYLALVW.
OrthoDBiEOG6RNQDX.
PhylomeDBiQ9HVJ9.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiRTCA_PSEAE
AccessioniPrimary (citable) accession number: Q9HVJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.