Reviewed, UniProtKB/Swiss-Prot Q9HVI7 (GLYA3_PSEAE)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 3
      Short name=Serine methylase 3
      Short name=SHMT 3
    EC=2.1.2.1

Gene names

Name:	glyA3
Ordered Locus Names:	PA4602

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine hydroxymethyltransferase 3 HAMAP MF_00051		PRO_0000113639
Amino acid modifications
	Modified residue	229	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HVI7-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: CACBABE0B548CF75	FASTA	417	45,157
	10 20 30 40 50 60 MFSRDLTLAR YDAELFAAME QEAQRQEEHI ELIASENYTS PAVMEAQGSV LTNKYAEGYP 70 80 90 100 110 120 HKRYYGGCEY VDIVEQLAID RAKQLFGADY ANVQPHAGSQ ANAAVYLALL SAGDTILGMS 130 140 150 160 170 180 LAHGGHLTHG ASVSSSGKLY NAVQYGIDAN GLIDYDEVER LAVEHKPKMI VAGFSAYSQV 190 200 210 220 230 240 LDFARFRAIA DKVGAYLFVD MAHVAGLVAA GVYPNPVPFA DVVTTTTHKT LRGPRGGLIL 250 260 270 280 290 300 ARANEEIEKK LNSAVFPSAQ GGPLEHVIAA KAVCFKEALQ PEFKTYQQQV LKNAQSMAQV 310 320 330 340 350 360 FLDRGFDVVS GGTQNHLFLL SLIKQDITGK DADAALGRAF ITVNKNSVPN DPRSPFVTSG 370 380 390 400 410 LRIGTPAVTT RGFKEAECRE LAGWICDILE NMGDESVVDG VREKVKAICA KFPVYGN

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG07990.1.
PIR	D83070.
RefSeq	NP_253292.1.
3D structure databases
SMR	Q9HVI7. Positions 1-416.
ModBase	Search...
Genome annotation databases
GeneID	881085.
GenomeReviews	Gene locus PA4602 in contig AE004091_GR.
KEGG	pae:PA4602.
NMPDR	fig|208964.1.peg.4602.
Organism-specific databases
PseudoCAP	PA4602.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG301263.
OMA	NINDEAV.
Enzyme and pathway databases
BioCyc	PAER208964:PA4602-MONOMER.
BRENDA	2.1.2.1. 354.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA3_PSEAE

Accession

Primary (citable) accession number: Q9HVI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)