Q9HVH4 (RLMG_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	PA4617

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366472

Sequences

	Sequence		Length	Mass (Da)
	Q9HVH4 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C7D3DAFF97C09AFF	FASTA	374	40,476
	10 20 30 40 50 60 MPLFATPFAQ LDLVRQPEQD GEPLQAFDAA DEYLLNQLHE RGVTAQCRVL VLNDAFGALA 70 80 90 100 110 120 ASLAPHVQVT SSGDSHLGFL ALRKNLARNG LDLGSVRFVP ASETAVGPFD HVLVKVPKTL 130 140 150 160 170 180 ALLEEQLIRL HGQLAPGAQV VAAGMVKHLP RAAGDLLERY IGPMHASLAV KKARLLIAEA 190 200 210 220 230 240 AERPQPRSPY PTRYRLEQPP LTLLNHANVF CREGLDIGTR AFLPHLPRSL GALRAADLGC 250 260 270 280 290 300 GNGVLGIAYA LLNPQAELAL VDESYMAVQS ARENWRAALG ERPATFRADD GLAGQAAGSL 310 320 330 340 350 360 DLVLCNPPFH QQQVVGDFLA WRMFLQARDA LAAGGELWIV GNRHLGYHAK LKRLFRGVEQ 370 VAANPKFVIL KAGK

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG08005.1.
PIR	A83070.
RefSeq	NP_253307.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9HVH4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	881134.
GenomeReviews	Gene locus PA4617 in contig AE004091_GR.
KEGG	pae:PA4617.
NMPDR	fig|208964.1.peg.4617.
PATRIC	19843985. VBIPseAer58763_4834.
Organism-specific databases
PseudoCAP	PA4617.
Phylogenomic databases
HOGENOM	HBG321931.
OMA	NRHLGYH.
ProtClustDB	CLSK868782.
Enzyme and pathway databases
BioCyc	PAER208964:PA4617-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_PSEAE

Accession

Primary (citable) accession number: Q9HVH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program